| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA3816081 | Binding | Induction of human 14-3-3sigma binding to TASK-3 using FAM-370RRK-pS-V374-COOH by fluorescence polarization assay relative to control | Homo sapiens | 1 | ALA3813646 | single protein format | Scientific Literature | |
| 2. | ALA3816082 | Binding | Inhibition of 14-3-3sigma (unknown origin) assessed as reduction in 14-3-3sigma- mediated increase in MMP1 mRNA by cell based assay | Homo sapiens | 1 | ALA3813646 | cell-based format | Scientific Literature | |
| 3. | ALA1912841 | Binding | Binding affinity to histidine-tagged human recombinant 14-3-3sigma by transferred NOESY analysis | Homo sapiens | 1 | ALA1909530 | single protein format | Scientific Literature | |
| 4. | ALA1912842 | Binding | Inhibition of 14-3-3sigma binding domain in human Ba/F3 cells expressing imatibib-sensitive wild type Bcr-Abl construct assessed as inhibition of 14-3-3sigma-c-Abl interaction | Homo sapiens | 3 | ALA1909530 | cell-based format | Scientific Literature | |
| 5. | ALA1912843 | Binding | Inhibition of 14-3-3sigma binding domain in human Ba/F3 cells expressing imatibib-resistant Bcr-Abl T315I mutant construct assessed as inhibition of 14-3-3sigma-c-Abl interaction | Homo sapiens | 2 | ALA1909530 | cell-based format | Scientific Literature | |
| 6. | ALA1912844 | Binding | Inhibition of 14-3-3sigma in human Ba/F3 cells expressing imatinib-sensitive wild type Bcr-Abl construct assessed as release of full-length c-Abl from cytoplasmic complex with 14-3-3sigma at 5 uM after 24 hrs by immunoprecipitation/immunoblot technique | Homo sapiens | 1 | ALA1909530 | cell-based format | Scientific Literature | |
| 7. | ALA1912845 | Binding | Inhibition of 14-3-3sigma in human Ba/F3 cells expressing wild type Bcr-Abl construct assessed as release of full-length c-Abl from cytoplasmic complex with 14-3-3sigma at 1 uM after 24 hrs by immunoprecipitation/immunoblot technique | Homo sapiens | 1 | ALA1909530 | cell-based format | Scientific Literature | |
| 8. | ALA1912846 | Binding | Inhibition of 14-3-3sigma in human Ba/F3 cells expressing imatinib-sensitive wild type Bcr-Abl construct assessed as nuclear import of full-length c-Abl at 5 uM after 24 hrs by immunoprecipitation/immunoblot technique | Homo sapiens | 1 | ALA1909530 | cell-based format | Scientific Literature | |
| 9. | ALA1912847 | Binding | Inhibition of 14-3-3sigma in human Ba/F3 cells expressing wild type Bcr-Abl construct assessed as nuclear import of full-length c-Abl at 1 uM after 24 hrs by immunoprecipitation/immunoblot technique | Homo sapiens | 1 | ALA1909530 | cell-based format | Scientific Literature | |
| 10. | ALA1912848 | Binding | Inhibition of 14-3-3sigma in human Ba/F3 cells expressing imatinib-sensitive wild type Bcr-Abl construct assessed as relocation of full-length c-Abl to mitochondrial membranes at 5 uM after 24 hrs by immunoprecipitation/immunoblot technique | Homo sapiens | 1 | ALA1909530 | cell-based format | Scientific Literature | |
| 11. | ALA1912849 | Binding | Inhibition of 14-3-3sigma in human Ba/F3 cells expressing imatinib-sensitive wild type Bcr-Abl construct assessed as cleaved c-Abl at mitochondrial membranes at 5 uM after 24 hrs by immunoprecipitation/immunoblot technique | Homo sapiens | 1 | ALA1909530 | cell-based format | Scientific Literature | |
| 12. | ALA1913023 | Binding | Inhibition of 14-3-3sigma in human Ba/F3 cells expressing imatinib-sensitive wild type Bcr-Abl construct assessed as cell survival | Homo sapiens | 1 | ALA1909530 | cell-based format | Scientific Literature | |
| 13. | ALA4118584 | Binding | Binding affinity to SFN in human NCI-H23 cells at 1 uM by mass spectrometry based pull down assay | Homo sapiens | 1 | ALA4118021 | cell-based format | Scientific Literature | |
| 14. | ALA4229668 | Binding | Inhibition of TMR-GG-RLSHpSSLPG-NH2 binding to recombinant human GST-tagged 14-3-3-sigma preincubated for 30 mins followed by TMR-GG-RLSHpSSLPG-NH2 addition and measured after 60 mins by fluorescence polarization assay | Homo sapiens | 5 | ALA4229326 | single protein format | Scientific Literature | |
| 15. | ALA4618546 | Binding | Binding affinity to 6x-His tagged 14-3-3-sigma (unknown origin) expressed in Escherichia coli BL21 (DE3) at 500 uM in presence of p53pT387 peptide by WaterLOGSY NMR spectroscopy | Homo sapiens | 1 | ALA4616674 | assay format | Scientific Literature | |
| 16. | ALA4618547 | Binding | Binding affinity to 15N2H labeled and 6x-His tagged 14-3-3-sigma (unknown origin) expressed in Escherichia coli assessed as induction of broadening (intensity decrease) and chemical shift value perturbation at 2000 uM in absence of of p53pT387 peptide by 1H-15N TROSY-HSQC NMR spectroscopy | Homo sapiens | 1 | ALA4616674 | assay format | Scientific Literature | |
| 17. | ALA4618548 | Binding | Binding affinity to 15N2H labeled and 6x-His tagged 14-3-3-sigma (unknown origin) expressed in Escherichia coli assessed as induction of broadening (intensity decrease) and chemical shift value perturbation at 2000 uM in presence of p53pT387 peptide by 1H-15N TROSY-HSQC NMR spectroscopy | Homo sapiens | 1 | ALA4616674 | assay format | Scientific Literature | |
| 18. | ALA4618549 | Binding | Binding affinity to 6x-His tagged 14-3-3-sigma (unknown origin) expressed in Escherichia coli at 1 mM by fluorescence polarization assay | Homo sapiens | 1 | ALA4616674 | assay format | Scientific Literature | |
| 19. | ALA4618550 | Binding | Binding affinity to 6x-His tagged 14-3-3-sigma (unknown origin) expressed in Escherichia coli at 1 mM by SPR assay | Homo sapiens | 1 | ALA4616674 | assay format | Scientific Literature | |
| 20. | ALA4618551 | Binding | Binding affinity to 6x-His tagged 14-3-3-sigma (unknown origin) expressed in Escherichia coli BL21 (DE3) in presence of acetylated p53pT387 peptide by crystallography | Homo sapiens | 3 | ALA4616674 | assay format | Scientific Literature |
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