| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA1913304 | B | Inhibition of histidine-tagged human SENP1 catalytic domain at 20 uM preincubated for 10 mins by SUMO-CHOP-PLA2 reporter fluorescence assay | Homo sapiens | 27 | ALA1909559 | single protein format | Scientific Literature | |
| 2. | ALA1913305 | B | Inhibition of histidine-tagged human SENP1 catalytic domain preincubated for 10 mins by SUMO-CHOP-PLA2 reporter fluorescence assay | Homo sapiens | 11 | ALA1909559 | single protein format | Scientific Literature | |
| 3. | ALA1913306 | B | Inhibition of histidine-tagged human SENP1 catalytic domain assessed as cleavage of fusion protein SUMO-deltaRanGAP cleavage at 50 uM preincubated for 10 mins by SDS-PAGE | Homo sapiens | 2 | ALA1909559 | single protein format | Scientific Literature | |
| 4. | ALA2114742 | F | PubChem BioAssay. Dose response confirmation of small molecule inhibitors of the catalytic domain of the SUMO protease, SENP1 in a kinetic FRET assay. (Class of assay: confirmatory) | Homo sapiens | 177 | ALA1201862 | assay format | PubChem BioAssays | |
| 5. | ALA2114823 | F | PubChem BioAssay. Dose response confirmation of small molecule inhibitors of the catalytic domain of the SUMO protease, SENP1 in a FRET assay. (Class of assay: confirmatory) | Homo sapiens | 177 | ALA1201862 | assay format | PubChem BioAssays | |
| 6. | ALA2162807 | B | Inhibition of recombinant His-tagged SENP1 catalytic domain expressed in Escherichia coli BL21(DE3) using His-tagged deltaRanGAP-SUMO2 as substrate at 50 uM incubated for 10 mins prior to substrate addition measured after 45 mins by SDS-PAGE analysis | 38 | ALA2157867 | assay format | Scientific Literature | ||
| 7. | ALA2162808 | B | Inhibition of recombinant His-tagged SENP1 catalytic domain expressed in Escherichia coli BL21(DE3) using His-tagged deltaRanGAP-SUMO2 as substrate incubated for 10 mins prior to substrate addition measured after 45 mins by SDS-PAGE analysis | 10 | ALA2157867 | assay format | Scientific Literature | ||
| 8. | ALA2187435 | B | Inhibition of SENP1 catalytic domain endopeptidase activity assessed as release of fluorescent AMC from SUMO-1-AMC preincubated for 10 mins before substrate addition by fluorescence assay | 2 | ALA2177099 | single protein format | Scientific Literature | ||
| 9. | ALA2187436 | B | Inhibition of SENP1 catalytic domain endopeptidase activity assessed as release of fluorescent AMC from SUMO-1-AMC preincubated at 50 uM for 10 mins before substrate addition by fluorescence assay | 3 | ALA2177099 | single protein format | Scientific Literature | ||
| 10. | ALA2187437 | B | Inhibition of SENP1 catalytic domain endopeptidase activity assessed as release of fluorescent AMC from SUMO-1-AMC preincubated at 100 uM for 10 mins before substrate addition by fluorescence assay | 1 | ALA2177099 | single protein format | Scientific Literature | ||
| 11. | ALA2187438 | B | Binding affinity to SENP1 in human HeLa cells after 1 hr by affinity chromatography/Western blot analysis | Homo sapiens | 2 | ALA2177099 | cell-based format | Scientific Literature | |
| 12. | ALA2400583 | B | Inhibition of core catalytic domain of SENP1 (unknown origin) using SUMO3-EKL as substrate | Homo sapiens | 1 | ALA2396580 | single protein format | Scientific Literature | |
| 13. | ALA3801657 | B | Inhibition of human recombinant His-tagged SENP1 (419 to 644 residues) expressed in Escherichia coli BL21 (DE3) using RanGAP-SUMO as substrate incubated for 10 mins prior to substrate addition followed by further 25 mins incubation by coomassie blue staining based SDS-PAGE/Odyssey infrared scanning assay | Homo sapiens | 1 | ALA3797135 | assay format | Scientific Literature | |
| 14. | ALA3801659 | B | Inhibition of human recombinant His-tagged SENP1 (419 to 644 residues) expressed in Escherichia coli BL21 (DE3) at 20 uM using RanGAP-SUMO as substrate incubated for 10 mins prior to substrate addition followed by further 25 mins incubation by coomassie blue staining based SDS-PAGE/Odyssey infrared scanning assay | Homo sapiens | 1 | ALA3797135 | assay format | Scientific Literature | |
| 15. | ALA3801660 | B | Inhibition of human recombinant His-tagged SENP1 (419 to 644 residues) expressed in Escherichia coli BL21 (DE3) using RanGAP-SUMO as substrate incubated for 10 mins prior to substrate addition followed by further 25 mins incubation by krypton dye based fluorescence imaging method | Homo sapiens | 1 | ALA3797135 | assay format | Scientific Literature | |
| 16. | ALA3864311 | B | Inhibition of SENP1 catalytic domain (419 to 644 residues) (unknown origin) expressed in Escherichia coli BL21 (DE3) assessed as reduction in deSUMOylation preincubated for 10 mins followed by addition of RanGAP-SUMO as substrate measured after 25 mins by coomassie staining-based SDS-PAGE analysis | Homo sapiens | 25 | ALA3864126 | assay format | Scientific Literature | |
| 17. | ALA4479671 | B | Inhibition of recombinant human 6His-tagged SENP1 catalytic domain expressed in Escherichia coli preincubated for 15 mins followed by addition of SUMO1-AMC as substrate measured after 1 hr by fluorescence analysis | Homo sapiens | 5 | ALA4477251 | assay format | Scientific Literature | |
| 18. | ALA4479672 | B | Inhibition of full-length recombinant human 6His-tagged SENP1 expressed in Escherichia coli BL21 (DE3) preincubated for 15 mins followed by addition of SUMO1-AMC as substrate measured after 1 hr by fluorescence analysis | Homo sapiens | 5 | ALA4477251 | assay format | Scientific Literature | |
| 19. | ALA4479673 | B | Binding affinity to recombinant human 6His-tagged SENP1 catalytic domain expressed in Escherichia coli | Homo sapiens | 1 | ALA4477251 | assay format | Scientific Literature | |
| 20. | ALA4479674 | B | Binding affinity to full-length recombinant human 6His-tagged SENP1 expressed in Escherichia coli BL21 (DE3) | Homo sapiens | 1 | ALA4477251 | assay format | Scientific Literature |
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