# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA1226745 | F | Antagonist activity at mouse S1P1 receptor expressed in CHO cells assessed as inhibition of S1P-induced [35S]GTPgamma binding | Mus musculus | 1 | ALA1221186 | cell-based format | Scientific Literature | |
2. | ALA1920394 | F | Agonist activity at mouse S1P1 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated cAMP production after 1 hr by HTRF assay | Mus musculus | 2 | ALA1914334 | cell-based format | Scientific Literature | |
3. | ALA2049907 | F | Agonist activity against mouse S1P1 assessed as accumulation of cAMP by HTRF assay | Mus musculus | 2 | ALA2046302 | assay format | Scientific Literature | |
4. | ALA3380157 | F | Agonist activity at mouse S1P1 receptor assessed as cAMP accumulation by HTRF assay | Mus musculus | 2 | ALA3352004 | assay format | Scientific Literature | |
5. | ALA3380158 | F | Agonist activity at mouse S1P1 receptor assessed as cAMP accumulation by HTRF assay relative to S1P | Mus musculus | 2 | ALA3352004 | assay format | Scientific Literature | |
6. | ALA3380990 | F | Agonist activity at mouse S1P1 receptor assessed as cAMP accumulation by HTRF assay | Mus musculus | 1 | ALA3352115 | assay format | Scientific Literature | |
7. | ALA3380991 | F | Agonist activity at mouse S1P1 receptor assessed as cAMP accumulation by HTRF assay relative to S1P | Mus musculus | 1 | ALA3352115 | assay format | Scientific Literature | |
8. | ALA3404801 | F | Agonist activity at mouse S1P1 receptor assessed as change in cAMP level by homogeneous time resolved fluorescence cyclase assay | Mus musculus | 1 | ALA3399954 | single protein format | Scientific Literature | |
9. | ALA3404802 | F | Agonist activity at mouse S1P1 receptor assessed as change in cAMP level by homogeneous time resolved fluorescence cyclase assay relative to S1P | Mus musculus | 1 | ALA3399954 | single protein format | Scientific Literature | |
10. | ALA3607262 | B | Antagonist activity against mouse S1P1 receptor expressed in human U2OS cells co-expressing GFP assessed as inhibition of S1P-induced receptor translocation | Mus musculus | 2 | ALA3603779 | cell-based format | Scientific Literature | |
11. | ALA3888306 | B | β-Arrestin Assay: Assay procedures: OCC culture solution was preheated in water tank at 37° C. Cells were taken out from liquor nitrogen tank, left on the dry-ice, and dissolved in water tank at 37° C. 0.5 ml OCC culture solution was added and blended slowly. The cells were diluted in 11.5 mL OCC culture solution, and 100 μL cell suspension (containing 8333 cells) was added to each well of the 96-well plate. The 96-well plate was incubated for 8 hours under 5% CO2 at 37° C., and 10 μL test compound dissolved in DMSO was added in; 10 μL DMSO was used as negative control. After the plate was incubated for 90 min at 37° C., 55 μL detection solution was added to each well. Then the results were read out by EnVision after the cell plate was incubated for 90 min at room temperature. | 8 | ALA3886647 | assay format | BindingDB Database |