| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA1932236 | B | Inhibition of NP40-inactivated Influenza A virus (A/Brisbane/59/2007(H1N1)) neuraminidase after 10 mins by dose responsive study | Influenza A virus (A/Brisbane/59/2007(H1N1)) | 2 | ALA1926680 | single protein format | Scientific Literature | |
| 2. | ALA3706058 | B | Enzyme Assay: Virus propagation: Influenza A/Brisbane/59/2007 (H1N1) virus was propagated in Madin-Darby Canine Kidney (MDCK) cells. Seed virus was inoculated into 10 confluent 75 cm2 monolayers which were incubated at 37° C. for 2-3 days and harvested when full cytopathic effect was observed. Virus purification: The pooled cell lysates were frozen and thawed and clarified by centrifugation at 3000 g for 20 mM. The supernatant was subjected to ultracentrifugation at 25,000 rpm for 90 min and the virus containing pellet was resuspended in 2 mL of MegaVir medium (Hyclone). Virus concentration was assessed by hemagglutination (HA) at 40,960 HA units. Virus inactivation: NP40 (Fluka) was added to the purified influenza virus at a final concentration of 0.2% and the mixture was incubated at room temperature for a total of 3 hours and at 4° C. for 6 h. The inactivation of the virus was confirmed by the Tissue Culture Infectious Dose assay. | 4 | ALA3638996 | assay format | BindingDB Database | ||
| 3. | ALA4120984 | B | Inhibition of Influenza A virus (A/Fukui/45/04(H3N2)) neuraminidase E119V mutant activity using 4-MUNANA as substrate measured every min for 60 mins by fluorescence assay | Influenza A virus (A/Fukui/45/2004(H3N2)) | 6 | ALA4118102 | single protein format | Scientific Literature | |
| 4. | ALA4120985 | B | Inhibition of Influenza A virus (A/Fukui/45/04(H3N2)) neuraminidase E119V mutant activity using 4-MUNANA as substrate preincubated for 60 mins followed by substrate addition measured every min for 60 mins by fluorescence assay | Influenza A virus (A/Fukui/45/2004(H3N2)) | 6 | ALA4118102 | single protein format | Scientific Literature | |
| 5. | ALA4121007 | B | Ratio of IC50 for Influenza A virus (A/Fukui/45/04(H3N2)) neuraminidase E119V mutant measured every min for 60 mins to IC50 for Influenza A virus (A/Fukui/45/04(H3N2)) neuraminidase E119V mutant preincubated for 60 mins followed by substrate addition measured every min for 60 mins | Influenza A virus (A/Fukui/45/2004(H3N2)) | 6 | ALA4118102 | single protein format | Scientific Literature | |
| 6. | ALA4254784 | B | Inhibition of Influenza A virus (A/Vietnam/1194/2004 (H5N1)) recombinant neuraminidase E119A mutant expressed in HEK293 cells pre-incubated for 10 mins before 4-MUNANA substrate addition by fluorometry | Influenza A virus (A/Brisbane/10/2007(H3N2)) | 3 | ALA4251642 | cell-based format | Scientific Literature | |
| 7. | ALA4254785 | B | Inhibition of Influenza A virus (A/Vietnam/1194/2004 (H5N1)) recombinant neuraminidase R292K mutant expressed in HEK293 cells pre-incubated for 10 mins before 4-MUNANA substrate addition by fluorometry | Influenza A virus (A/Brisbane/59/2007(H1N1)) | 3 | ALA4251642 | cell-based format | Scientific Literature | |
| 8. | ALA4254787 | B | Inhibition of Influenza A virus (A/Shanghai/01/2014(H7N9)) recombinant neuraminidase R292K mutant expressed in HEK293 cells pre-incubated for 10 mins before 4-MUNANA substrate addition by fluorometry | Influenza A virus (A/Shanghai/01/2014(H7N9)) | 3 | ALA4251642 | cell-based format | Scientific Literature |
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