| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA1937910 | B | Inhibition of recombinant Staphylococcus aureus NCTC 8325 peptidase activity of clpP expressed in Escherichia coli BL21 at 10 to 40 uM after 10 mins using SLY-AMC as substrate by fluorescence assay | Staphylococcus aureus | 2 | ALA1933002 | assay format | Scientific Literature | |
| 2. | ALA1937911 | B | Inhibition of recombinant Staphylococcus aureus NCTC 8325 peptidase activity of clpP expressed in Escherichia coli BL21 after 10 mins using SLY-AMC as substrate by fluorescence assay | Staphylococcus aureus | 3 | ALA1933002 | assay format | Scientific Literature | |
| 3. | ALA1937912 | B | Inhibition of recombinant Staphylococcus aureus NCTC 8325 peptidase activity of clpP expressed in Escherichia coli BL21 at 200 to 800 uM after 10 mins using SLY-AMC as substrate by fluorescence assay | Staphylococcus aureus | 3 | ALA1933002 | assay format | Scientific Literature | |
| 4. | ALA1937913 | B | Displacement of U1P from recombinant Staphylococcus aureus NCTC 8325 clpP expressed in Escherichia coli BL21 after 1 hr using rhodamine-azide by SDS PAGE fluorescence analysis | Staphylococcus aureus | 3 | ALA1933002 | assay format | Scientific Literature | |
| 5. | ALA4321958 | B | Activation of ClpP in stationary phase methicillin-resistant Staphylococcus aureus ATCC 33591 assessed as reduction in bacterial load after 2 days relative to control | Staphylococcus aureus | 1 | ALA4321783 | single protein format | Scientific Literature | |
| 6. | ALA4404284 | B | Inhibition of N-terminal His6-sumo-tagged full length Staphylococcus aureus ClpP expressed in Escherichia coli BL2 (DE3) at 10 uM pre-incubated for 10 mins before Suc-LY-AMC addition and measured after 1 hr by fluorescence based assay relative to control | Staphylococcus aureus | 42 | ALA4402562 | assay format | Scientific Literature | |
| 7. | ALA4404285 | B | Inhibition of N-terminal His6-sumo-tagged full length Staphylococcus aureus ClpP expressed in Escherichia coli BL2 (DE3) pre-incubated for 10 mins before Suc-LY-AMC addition and measured after 1 hr by fluorescence based assay | Staphylococcus aureus | 7 | ALA4402562 | assay format | Scientific Literature | |
| 8. | ALA4404286 | B | Inhibition of N-terminal His6-sumo-tagged full length Staphylococcus aureus ClpP expressed in Escherichia coli BL2 (DE3) assessed as Kinact/Ki ratio pre-incubated for 10 mins before Suc-LY-AMC addition and measured after 1 hr by fluorescence based assay | Staphylococcus aureus | 7 | ALA4402562 | assay format | Scientific Literature | |
| 9. | ALA4404287 | B | Inhibition of ClpP in methicillin-resistant Staphylococcus aureus ATCC 33591 assessed as reduction in bacteria-induced hemolysis of sheep erythrocytes by measuring hemoglobin release incubated for 15 mins | Staphylococcus aureus | 7 | ALA4402562 | assay format | Scientific Literature | |
| 10. | ALA4404291 | B | Binding affinity to N-terminal His6-sumo-tagged full length Staphylococcus aureus ClpP expressed in Escherichia coli BL2 (DE3) assessed as melting temperature at 100 uM by differential scanning calorimetry analysis (Rvb = 50.8 degC) | Staphylococcus aureus | 1 | ALA4402562 | assay format | Scientific Literature | |
| 11. | ALA4404292 | B | Binding affinity to N-terminal His6-sumo-tagged full length Staphylococcus aureus ClpP expressed in Escherichia coli BL2 (DE3) assessed as shifting of melting peak of SaClpP by differential scanning calorimetry analysis | Staphylococcus aureus | 2 | ALA4402562 | assay format | Scientific Literature | |
| 12. | ALA4404293 | B | Inhibition of N-terminal His6-sumo-tagged full length Staphylococcus aureus ClpP expressed in Escherichia coli BL2 (DE3) at 3 uM pre-incubated for 10 mins before 200 uM to 1 mM Suc-LY-AMC addition and measured after 1 hr by fluorescence based assay relative to control | Staphylococcus aureus | 1 | ALA4402562 | assay format | Scientific Literature | |
| 13. | ALA4404294 | B | Binding affinity to N-terminal His6-sumo-tagged full length Staphylococcus aureus ClpP expressed in Escherichia coli BL2 (DE3) by ITC analysis | Staphylococcus aureus | 1 | ALA4402562 | assay format | Scientific Literature | |
| 14. | ALA4404295 | B | Binding affinity to N-terminal His6-sumo-tagged full length Staphylococcus aureus ClpP expressed in Escherichia coli BL2 (DE3) assessed as change in melting temperature at 100 uM by differential scanning calorimetry analysis | Staphylococcus aureus | 8 | ALA4402562 | assay format | Scientific Literature | |
| 15. | ALA4404296 | B | Inhibition of N-terminal His6-sumo-tagged full length Staphylococcus aureus ClpP expressed in Escherichia coli BL2 (DE3) at 20-fold molar excess pre-incubated for 10 mins before Suc-LY-AMC addition and measured after 1 hr followed by dialysis for 72 hrs by fluorescence based assay relative to control | Staphylococcus aureus | 1 | ALA4402562 | assay format | Scientific Literature | |
| 16. | ALA4404297 | B | Inhibition of N-terminal His6-sumo-tagged full length Staphylococcus aureus ClpP expressed in Escherichia coli BL2 (DE3) assessed as enzyme activity recovery level at 20-fold molar excess pre-incubated for 10 mins before Suc-LY-AMC addition and measured after 1 hr followed by dialysis up to 72 hrs by fluorescence based assay relative to control | Staphylococcus aureus | 7 | ALA4402562 | assay format | Scientific Literature | |
| 17. | ALA4404306 | B | Potency index, ratio of IC50 for BTZ to IC50 for test compound for inhibition of ClpP in methicillin-resistant Staphylococcus aureus ATCC 33591 assessed as reduction in bacteria-induced hemolysis of sheep erythrocytes by measuring hemoglobin release incubated for 15 mins | Staphylococcus aureus | 1 | ALA4402562 | assay format | Scientific Literature | |
| 18. | ALA4404309 | B | Inhibition of ClpP in wild type Staphylococcus aureus NCTC8325 assessed as reduction in bacteria-induced hemolysis of sheep erythrocytes by measuring hemoglobin release incubated for 15 mins | Staphylococcus aureus | 1 | ALA4402562 | assay format | Scientific Literature | |
| 19. | ALA4404311 | B | Inhibition of ClpP in Staphylococcus aureus NCTC8325 with complemented deltaclpP/pYJ335::clpP (deltaclpP+) assessed as reduction in bacteria-induced hemolysis of sheep erythrocytes by measuring hemoglobin release incubated for 15 mins | Staphylococcus aureus | 1 | ALA4402562 | assay format | Scientific Literature | |
| 20. | ALA4404318 | B | Induction of thermal stabilization of ClpP in methicillin-resistant Staphylococcus aureus ATCC 33591 assessed as protein stability up to 83 degC pre-incubated for 2 hrs by CETSA method | Staphylococcus aureus | 1 | ALA4402562 | single protein format | Scientific Literature |
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