| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA1942542 | B | Inhibition of KDM6B | 1 | ALA1938384 | single protein format | Scientific Literature | ||
| 2. | ALA2167517 | B | Inhibition of human KDM6B catalytic domain expressed in Escherichia coli using methyl lysine peptide substrate by AlphaScreen assay | Homo sapiens | 10 | ALA2163321 | assay format | Scientific Literature | |
| 3. | ALA2395586 | B | Binding affinity to recombinant 6xHis-tagged JMJD3 (unknown origin) expressed in Escherichia coli BL21 up to 10 uM by fluorescence polarization assay | Homo sapiens | 1 | ALA2390894 | assay format | Scientific Literature | |
| 4. | ALA2396333 | B | Inhibition of recombinant 6xHis-tagged JMJD3 (unknown origin) expressed in Escherichia coli BL21 | Homo sapiens | 3 | ALA2390894 | assay format | Scientific Literature | |
| 5. | ALA3112251 | B | Inhibition of recombinant JMJD3 (unknown origin) incubated for 15 mins prior to substrate addition by AlphaScreen method | Homo sapiens | 9 | ALA3108715 | single protein format | Scientific Literature | |
| 6. | ALA3624704 | B | Inhibition of KDM6B (unknown origin) using H3(21-44)K27Me3-GK-biotin substrate incubated for 5 mins by alpha screen assay | Homo sapiens | 32 | ALA3621091 | single protein format | Scientific Literature | |
| 7. | ALA3624714 | B | Inhibition of KDM6B (unknown origin) | Homo sapiens | 6 | ALA3621091 | single protein format | Scientific Literature | |
| 8. | ALA3757620 | B | Inhibition of JMJD3 (unknown origin) using biotinylated H3K27me3 peptide as substrate after 1 hr by AlphaLISA assay | Homo sapiens | 30 | ALA3751830 | single protein format | Scientific Literature | |
| 9. | ALA3757621 | B | Inhibition of JMJD3 (unknown origin) using biotinylated H3K27me3 peptide as substrate at 10 uM after 1 hr by AlphaLISA assay | Homo sapiens | 14 | ALA3751830 | single protein format | Scientific Literature | |
| 10. | ALA3772968 | B | Inhibition of KDM6B (unknown origin) by RFMS assay | Homo sapiens | 7 | ALA3769320 | single protein format | Scientific Literature | |
| 11. | ALA3776335 | B | Inhibition of KDM6B (unknown origin) expressed in Escherichia coli using H3 (21 to 44 residues) K27Me3-GGK-Biotin JMJD3 Cognate Ligand/10 uM alpha-ketoglutarate as substrate/cofactor preincubated for 5 mins followed by substrate addition measured after 5 mins by alphascreen assay | Homo sapiens | 6 | ALA3774347 | assay format | Scientific Literature | |
| 12. | ALA3776391 | B | Inhibition of KDM6B (unknown origin) using biotin-H3K27me3 (21 to 44 residues) as substrate preincubated for 15 mins followed by substrate addition measured after 5 mins by alphascreen assay | Homo sapiens | 3 | ALA3774338 | single protein format | Scientific Literature | |
| 13. | ALA3776393 | B | Inhibition of KDM6B (unknown origin) using biotin-H3K27me3 (21 to 44 residues) as substrate at 100 uM preincubated for 15 mins followed by substrate addition measured after 5 mins by alphascreen assay relative to control | Homo sapiens | 5 | ALA3774338 | single protein format | Scientific Literature | |
| 14. | ALA3778163 | B | Inhibition of human KDM6B catalytic domain using H3(20 to 36 residues)K27Me3 peptide as substrate assessed as demethylation of substrate by Rapidfire mass spectrometric analysis | Homo sapiens | 18 | ALA3774367 | single protein format | Scientific Literature | |
| 15. | ALA3791195 | B | Inhibition of KDM6B (unknown origin) after 24 hrs by alphascreen assay | Homo sapiens | 1 | ALA3784917 | single protein format | Scientific Literature | |
| 16. | ALA3791436 | B | Inhibition of KDM6B (unknown origin) preincubated for 15 mins followed by histone substrate addition measured after 1 hr by alphascreen assay | Homo sapiens | 1 | ALA3784917 | single protein format | Scientific Literature | |
| 17. | ALA3791445 | B | Inhibition of human C-terminal FLAG-tagged KDM6B (1043 residues) expressed in baculovirus infected sf9 cells using H3K27me3 (21 to 44 residues) substrate preincubated for 10 mins followed by substrate addition measured after 45 mins by TR-FRET assay | Homo sapiens | 1 | ALA3784917 | cell-based format | Scientific Literature | |
| 18. | ALA3789215 | B | Inhibition of human C-terminal FLAG-tagged KDM6B (1043 residues) expressed in baculovirus infected sf9 cells using H3K27me3 as substrate preincubated for 10 mins followed by substrate addition measured after 10 mins by AlphaLisa assay | Homo sapiens | 3 | ALA3784917 | cell-based format | Scientific Literature | |
| 19. | ALA3795794 | B | Inhibition of KDM6B (unknown origin) using substrate peptide/ascorbate/2-OG/ Fe(2) as substrate preincubated for 15 mins followed by addition of substrate peptide/ ascorbate/2-OG/ Fe(2) measured after 1 hr by AlphaLISA method | Homo sapiens | 1 | ALA3792324 | single protein format | Scientific Literature | |
| 20. | ALA4193857 | B | Inhibition of KDM6B (unknown origin) using biotinylated H3K27me3 peptide and alpha-ketoglutarate as substrate pretreated for 1 hr followed by substrate addition measured after 20 mins by LANCE TR-FRET assay | Homo sapiens | 1 | ALA4190335 | single protein format | Scientific Literature |
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