| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA1942543 | B | Inhibition of KDM7B | 1 | ALA1938384 | single protein format | Scientific Literature | ||
| 2. | ALA2034758 | B | Inhibition of human N-terminal His6-thioredoxin tagged PHF8 expressed in Escherichia coli using 2-OG as substrate incubated for 15 mins prior to substrate addition measured after 20 mins by MALDI-TOF analysis | Homo sapiens | 3 | ALA2029258 | assay format | Scientific Literature | |
| 3. | ALA2167523 | B | Inhibition of human PHF8 expressed in Escherichia coli using methyl lysine peptide substrate by AlphaScreen assay | Homo sapiens | 10 | ALA2163321 | assay format | Scientific Literature | |
| 4. | ALA2427607 | B | Inhibition of KDM7B (unknown origin) by alphascreen assay | Homo sapiens | 1 | ALA2424657 | single protein format | Scientific Literature | |
| 5. | ALA2427611 | B | Inhibition of KDM7B (unknown origin) using H3K4me3K9me2 and 2-oxoglutarate as substrate after 1 hr by matrix-assisted laser desorption ionization time of flight mass spectrometry | Homo sapiens | 12 | ALA2424657 | single protein format | Scientific Literature | |
| 6. | ALA3591679 | B | Inhibition of JHDM1F (unknown origin) using H3K9mel peptide | Homo sapiens | 17 | ALA3588787 | single protein format | Scientific Literature | |
| 7. | ALA3782533 | B | Inhibition of human PHF8 preincubated for 10 mins followed by substrate addition by AlphaLISA assay | Homo sapiens | 6 | ALA3779836 | single protein format | Scientific Literature | |
| 8. | ALA4035842 | B | Inhibition of recombinant human N-terminal FLAG-tagged KDM7B expressed in baculovirus infected Sf9 insect cells using H3K9me1 peptide as substrate after 30 to 45 mins by TR-FRET assay | Homo sapiens | 2 | ALA4033700 | cell-based format | Scientific Literature | |
| 9. | ALA4122976 | B | Displacement of C-terminally biotinylated-H3K4me3 (1 to 21 residues) peptide from KDM7B (PHD) (unknown origin) preincubated for 15 mins followed by peptide addition measured after 1 hr by luminescence-based AlphaScreen assay | Homo sapiens | 15 | ALA4118181 | single protein format | Scientific Literature | |
| 10. | ALA4122982 | B | Displacement of C-terminally biotinylated-H3K4me3 (1 to 21 residues) peptide from KDM7B (PHD-JmjC) (unknown origin) preincubated for 15 mins followed by peptide addition measured after 1 hr by luminescence-based AlphaScreen assay | Homo sapiens | 3 | ALA4118181 | single protein format | Scientific Literature | |
| 11. | ALA4122983 | B | Displacement of C-terminally biotinylated-H3K4me3 (1 to 21 residues) peptide from KDM7B (PHD-JmjC) (unknown origin) at 100 uM preincubated for 15 mins followed by peptide addition measured after 1 hr by luminescence-based AlphaScreen assay | Homo sapiens | 3 | ALA4118181 | single protein format | Scientific Literature | |
| 12. | ALA4122984 | B | Inhibition of KDM7B (PHD-JmjC) (unknown origin) demethylation activity at 100 uM preincubated for 10 mins followed by H3(1-15)K4me3K9me2 peptide substrate addition by MALDI-TOF-MS analysis | Homo sapiens | 7 | ALA4118181 | single protein format | Scientific Literature | |
| 13. | ALA4193858 | B | Inhibition of PHF8 (unknown origin) using biotinylated H3K9me1 peptide and alpha-ketoglutarate as substrate measured after 20 mins by LANCE TR-FRET assay | Homo sapiens | 1 | ALA4190335 | single protein format | Scientific Literature | |
| 14. | ALA4257000 | B | Inhibition of recombinant human N-terminal FLAG-tagged PHF8 expressed in baculovirus infected Sf9 cells using H3K9mel-biotin labelled peptide as substrate measured after 15 mins by Lance TR-FRET assay | Homo sapiens | 55 | ALA4256948 | cell-based format | Patent Bioactivity Data | |
| 15. | ALA4316552 | B | Inhibition of Flag-tagged PHF8 (unknown origin) expressed in Escherichia coli BL21 (DE3) assessed as formaldehyde release using core histone as substrate preincubated with enzyme for 10 mins followed by incubation with substrate for 1 hr measured following 2 hrs incubation with ammonium acetate/acetoacetanilide by fluorescence based analysis | Homo sapiens | 1 | ALA4312042 | assay format | Scientific Literature | |
| 16. | ALA4377355 | B | Binding affinity to PHF8 (unknown origin) assessed as induction of thermal shifts at 20 uM measured for 25 mins by SYPRO orange dye thermal shift assay | Homo sapiens | 1 | ALA4376809 | single protein format | Scientific Literature | |
| 17. | ALA4424794 | B | Inhibition of human N-terminal FLAG-tagged KDM7B (2 to 525 residues) expressed in baculovirus infected Sf9 insect cells at 50 uM using H3(1-21)K9(Me2)-GGK(Biotin)/2-OG as substrate measured after 1 hr by Alphalisa assay relative to control | Homo sapiens | 4 | ALA4422684 | cell-based format | Scientific Literature | |
| 18. | ALA4424795 | B | Inhibition of human N-terminal FLAG-tagged KDM7B (2 to 525 residues) expressed in baculovirus infected Sf9 insect cells at 25 uM using H3(1-21)K9(Me2)-GGK(Biotin)/2-OG as substrate measured after 1 hr by Alphalisa assay relative to control | Homo sapiens | 4 | ALA4422684 | cell-based format | Scientific Literature | |
| 19. | ALA4424800 | B | Inhibition of human N-terminal FLAG-tagged KDM7B (2 to 525 residues) expressed in baculovirus infected Sf9 insect cells using H3(1-21)K9(Me2)-GGK(Biotin)/2-OG as substrate measured after 1 hr by Alphalisa assay | Homo sapiens | 2 | ALA4422684 | cell-based format | Scientific Literature | |
| 20. | ALA4427169 | B | Inhibition of KDM7B (unknown origin) | Homo sapiens | 1 | ALA4425149 | single protein format | Scientific Literature |
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