# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA1942913 | Binding | Activation of EIF2alpha kinase in mouse KLN205 cells assessed as reduction in eIF2-GTP-initiator methionine tRNA ternary complex measuring increase in firefly/renilla luciferase ratio at 2.5 to 80 uM after 16 hrs by dual luciferase reporter gene assay relative to control | Mus musculus | 1 | ALA1938338 | cell-based format | Scientific Literature | |
2. | ALA1942914 | Binding | Activation of EIF2alpha kinase in mouse KLN205 cells assessed as CHOP mRNA expression at 20 uM after 5 hrs by PCR analysis | Mus musculus | 4 | ALA1938338 | cell-based format | Scientific Literature | |
3. | ALA1943306 | Binding | Activation of EIF2alpha kinase in mouse KLN205 cells assessed as phosphorylation of eIF2alpha at 20 uM after 5 hrs by Western blot analysis | Mus musculus | 4 | ALA1938338 | cell-based format | Scientific Literature | |
4. | ALA1943307 | Binding | Activation of EIF2alpha kinase in mouse KLN205 cells assessed as CHOP protein expression at 20 uM after 5 hrs by Western blot analysis | Mus musculus | 4 | ALA1938338 | cell-based format | Scientific Literature | |
5. | ALA1943309 | Binding | Activation of EIF2alpha kinase in mouse KLN205 cells assessed as reduction in eIF2-GTP-initiator methionine tRNA ternary complex measuring increase in firefly/renilla luciferase ratio at 5 uM after 16 hrs by dual luciferase reporter gene assay relative to control | Mus musculus | 1 | ALA1938338 | cell-based format | Scientific Literature | |
6. | ALA1943310 | Binding | Activation of EIF2alpha kinase in mouse KLN205 cells assessed as reduction in eIF2-GTP-initiator methionine tRNA ternary complex measuring increase in firefly/renilla luciferase ratio at 20 uM after 16 hrs by dual luciferase reporter gene assay relative to control | Mus musculus | 1 | ALA1938338 | cell-based format | Scientific Literature | |
7. | ALA4012848 | Binding | Activation of HRI in mouse KLN cells transfected with 5'UTR of ATF-4 mRNA fused firefly luciferase mRNA and 5'UTR lacking uORF fused renila luciferase mRNA assessed as increase in eIF2alpha phosphorylation by measuring ratio of firefly luciferase to renila luciferase at 1.25 uM after 16 hrs | Mus musculus | 24 | ALA4011623 | cell-based format | Scientific Literature | |
8. | ALA4012849 | Binding | Activation of HRI in mouse KLN cells transfected with 5'UTR of ATF-4 mRNA fused firefly luciferase mRNA and 5'UTR lacking uORF fused renila luciferase mRNA assessed as increase in eIF2alpha phosphorylation by measuring ratio of firefly luciferase to renila luciferase at 5 uM after 16 hrs | Mus musculus | 24 | ALA4011623 | cell-based format | Scientific Literature | |
9. | ALA4012851 | Binding | Activation of HRI in mouse KLN cells transfected with 5'UTR of ATF-4 mRNA fused firefly luciferase mRNA and 5'UTR lacking uORF fused renila luciferase mRNA assessed as increase in eIF2alpha phosphorylation by measuring ratio of firefly luciferase to renila luciferase after 16 hrs | Mus musculus | 23 | ALA4011623 | cell-based format | Scientific Literature |