#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA1943611	Binding	Inhibition of human MLKL in HL-60 cells lysate assessed as reduction of labeling of acyl-phosphate ATP probe at 100 nM	Homo sapiens	4	ALA1938230	cell-based format	Scientific Literature
2.	ALA3224036	Binding	Competitive binding affinity to Myc-tagged MLKL (unknown origin) transfected in human HeLa cells at 20 nM by Western blot analysis in presence of biotin-labeled positive probe	Homo sapiens	3	ALA3217478	cell-based format	Scientific Literature
3.	ALA4045624	Binding	Inhibition of MLKL conserved Lys1 (APVAIKVFK) in human PBMC at 1 uM	Homo sapiens	1	ALA4043168	single protein format	Scientific Literature
4.	ALA4045627	Binding	Inhibition of MLKL conserved Lys1 (APVAIKVFK) in human PBMC at 0.1 uM	Homo sapiens	1	ALA4043168	single protein format	Scientific Literature
5.	ALA4118411	Binding	Inhibition of MLKL in human SKCO1 cells at 1 uM after 4 hrs using biotin labeled APVAIKVFK probe by mass-spectrometric analysis relative to control	Homo sapiens	3	ALA4118021	cell-based format	Scientific Literature
6.	ALA4378753	Binding	Binding affinity to MLKL (unknown origin)	Homo sapiens	1	ALA4376828	single protein format	Scientific Literature
7.	ALA4378754	Binding	Binding affinity to human MLKL by kinome scan based method	Homo sapiens	2	ALA4376828	single protein format	Scientific Literature
8.	ALA4378756	Binding	Binding affinity to MLKL (unknown origin) assessed as increase in thermal stabilization at 10 uM incubated for 30 mins by thermal shift dye based qPCR analysis	Homo sapiens	2	ALA4376828	single protein format	Scientific Literature
9.	ALA4406996	Binding	Inhibition of MLKL phosphorylation in TNFalpha/smac mimetic/Z-VAD-fmk stimulated human HT-29 cells at 1 uM treated prior to TNFalpha/smac mimetic/Z-VAD-fmk stimulation and measured after 2 to 4 hrs by SDS-PAGE based immunoblot assay	Homo sapiens	1	ALA4406794	cell-based format	Scientific Literature
10.	ALA4804432	Binding	Inhibition of MLKL in human NCI-H929 cells by mass spectroscopic analysis	Homo sapiens	6	ALA4804281	cell-based format	Patent Bioactivity Data
11.	ALA4804614	Binding	Inhibition of MLKL in human NCI-H929 cells at 10 uM by mass spectroscopic analysis relative to control	Homo sapiens	6	ALA4804281	cell-based format	Patent Bioactivity Data
12.	ALA4817054	Binding	Inhibition of MLKL (unknown origin) polymerization	Homo sapiens	1	ALA4813992	single protein format	Scientific Literature
13.	ALA4842520	Binding	Inhibition of MLKL phosphorylation in Z-VAD-fmk (TSZ)-induced human HT-29 cells at 1 uM measured up to 4 hrs by immunoblotting analysis	Homo sapiens	2	ALA4842316	tissue-based format	Scientific Literature
14.	ALA4842523	Binding	Inhibition of MLKL phosphorylation in Z-VAD-fmk (TSZ)-induced human HT-29 cells measured at 4 hrs by immunoblotting analysis	Homo sapiens	2	ALA4842316	tissue-based format	Scientific Literature
15.	ALA4884782	Binding	MLKL(MLKLPHY1) Takeda global kinase panel	Homo sapiens	1	ALA4507327	single protein format	Donated Chemical Probes - SGC Frankfurt
16.	ALA4885073	Binding	MLKL(MLKLPHY1) Takeda global kinase panel	Homo sapiens	1	ALA4507326	single protein format	Donated Chemical Probes - SGC Frankfurt
17.	ALA4885361	Binding	MLKL(MLKLPHY1) Takeda global kinase panel	Homo sapiens	1	ALA4507327	single protein format	Donated Chemical Probes - SGC Frankfurt
18.	ALA5035742	Binding	Binding affinity to MLKL N-terminal executioner domain (2 to 154 residues)(unknown origin) assessed as dissociation constant by TROSY-HSQC NMR titration analysis	Homo sapiens	9	ALA5034032	single protein format	Scientific Literature
19.	ALA5035744	Binding	Binding affinity to MLKL N-terminal executioner domain (2 to 154 residues)(unknown origin) assessed as dissociation constant in presence of Nonyl-maltoside by TROSY-HSQC NMR titration analysis	Homo sapiens	1	ALA5034032	single protein format	Scientific Literature
20.	ALA5099515	Binding	Inhibition of MLKL phosphorylation in TSZ induced human HT-29 cells at 1 uM pretreated with compound followed by TSZ stimulation upto 6 hrs by immunoblotting analysis	Homo sapiens	1	ALA5096222	cell-based format	Scientific Literature