| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA2044968 | B | Inhibition of CXCR7 interaction with SDF1alpha | 7 | single protein format | Scientific Literature | |||
| 2. | ALA2013880 | B | Displacement of [125I]-CXCL12 from human CXCR7 expressed in HEK293 cells after 3 hrs | Homo sapiens | 24 | cell-based format | Scientific Literature | ||
| 3. | ALA2013881 | F | Agonist activity at human CXCR7 expressed in HEK293T cells co-expressing beta-arrestin2-YFP assessed as beta-arrestin2 recruitment after 1 hr by BRET assay | Homo sapiens | 3 | cell-based format | Scientific Literature | ||
| 4. | ALA2013882 | F | Agonist activity at human CXCR7 expressed in HEK293 cells assessed as reduction of receptor surface expression after 1 hr by ELISA based internalization assay | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 5. | ALA2013883 | F | Agonist activity at human CXCR7 expressed in HEK293 cells assessed as reduction of receptor surface expression at 10'-5 M after 1 hr by ELISA based internalization assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 6. | ALA2013884 | F | Agonist activity at CXCR7 | 2 | assay format | Scientific Literature | |||
| 7. | ALA2328020 | B | Antagonist activity at CXCR7 (unknown origin) assessed as inhibition of chemotaxis at 10 uM | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 8. | ALA2328508 | F | Antagonist activity at CXCR7 (unknown origin) assessed as inhibition of calcium flux at 10 uM | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 9. | ALA2328633 | B | Binding affinity to CXCR7 (unknown origin) at 10 uM by radioligand displacement assay | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 10. | ALA2384566 | B | Binding affinity to CXCR7 (unknown origin) | Homo sapiens | 4 | single protein format | Scientific Literature | ||
| 11. | ALA2384567 | B | Displacement of [125I]SDF-1alpha from CXCR7 (unknown origin) expressed in CHO cells after 1 hr by TopCount scintillation counting analysis | Homo sapiens | 21 | cell-based format | Scientific Literature | ||
| 12. | ALA5257248 | B | Antagonist activity at CXCR7 (unknown origin) | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 13. | ALA3583502 | B | Inhibition of [125I]SDF-1alpha binding to CXCR7 (unknown origin) expressed in CHO cell membranes incubated for 1 hr by radioligand displacement assay | Homo sapiens | 27 | cell-based format | Scientific Literature | ||
| 14. | ALA3583504 | B | Agonist activity at CXCR7 (unknown origin) expressed in HEK293E cells co-transfected with receptor-eYFP construct and beta-arrestin2-Rluc assessed as induction of receptor-mediated beta-arrestin recruitment by BRET assay | Homo sapiens | 4 | cell-based format | Scientific Literature | ||
| 15. | ALA3583505 | B | Agonist activity at wild type CXCR7 (unknown origin) expressed in HEK293E cells co-transfected with receptor-eYFP construct and beta-arrestin2-Rluc assessed as induction of receptor-mediated beta-arrestin recruitment by BRET assay | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 16. | ALA3583506 | B | Agonist activity at CXCR7 D179N mutant (unknown origin) expressed in HEK293E cells co-transfected with receptor-eYFP construct and beta-arrestin2-Rluc assessed as induction of receptor-mediated beta-arrestin recruitment by BRET assay | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 17. | ALA3583507 | B | Agonist activity at CXCR7 S198R mutant (unknown origin) expressed in HEK293E cells co-transfected with receptor-eYFP construct and beta-arrestin2-Rluc assessed as induction of receptor-mediated beta-arrestin recruitment by BRET assay | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 18. | ALA3583508 | B | Agonist activity at CXCR7 D275N mutant (unknown origin) expressed in HEK293E cells co-transfected with receptor-eYFP construct and beta-arrestin2-Rluc assessed as induction of receptor-mediated beta-arrestin recruitment by BRET assay | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 19. | ALA3855310 | B | Inhibition of anti-CXCR7 APC-antibody binding to CXCR7 in human MCF7 cells up to 10 uM preincubated for 30 mins followed by antibody addition by FACS Canto II cytofluorometric analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 20. | ALA3888966 | B | In Vitro Assay: CHO-K1 CXCR7 b-arrestin cells are detached from culture dishes with a cell dissociation buffer (Invitrogen, #13151-014) and collected in growing medium (F12 HAMS 90% (v/v)/FCS 10% (v/v), Penicilin/streptomycin 1% (v/v)). 5000 cells per well (in 20 μl) are seeded in a 384 well plate (white-walled, clear bottom; BD Falcon #353274). The plate is incubated at 37° C./5% CO2 for 24 hours. Medium is then replaced by 20 μl OPTIMEM (Invitrogen #31985) for 3 to 4 hours. Test compounds are dissolved at 10 mM in DMSO and serially diluted in DMSO to 200× of the final concentration for dose response curves. Compounds are then diluted 1:33.3 in HBSS1×. 5 μl/well of HBSS1×/20 mM HEPES/0.2% BSA are added to the assay plate followed by addition of 5 μl/well of diluted compounds. CXCL12 (Peprotech #300-28A) may be used as a reference agonist. The plate is incubated for 90 minutes at 37° C. 12 μl of detection reagent (Path Hunter Detection Kit, DiscoveRx, #93-0001) is transferred to the assay plate and to the plate is incubated for 1 hour at room temperature. Luminescent signal is read in a microplate reader (FLUOstar Optima, bmg). | 498 | cell-based format | BindingDB Database |
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