#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA2014263	B	Inhibition of Trypanosoma brucei PDEB1 using cAMP as substrate at 10 uM preincubated for 10 mins prior substrate addition by spectrophotometry	Trypanosoma brucei	11		single protein format	Scientific Literature
2.	ALA2014264	B	Inhibition of Trypanosoma brucei PDEB1 using cAMP as substrate at 100 uM preincubated for 10 mins prior substrate addition by spectrophotometry	Trypanosoma brucei	6		single protein format	Scientific Literature
3.	ALA2020843	B	Inhibition of Trypanosoma brucei PDEB1 at 100 uM	Trypanosoma brucei	27		single protein format	Scientific Literature
4.	ALA2438429	B	Inhibition of Trypanosoma brucei recombinant PDEB1 at 10 uM by spectrophotometric analysis relative to control	Trypanosoma brucei	20		single protein format	Scientific Literature
5.	ALA2438433	B	Inhibition of Trypanosoma brucei PDEB1	Trypanosoma brucei	2		single protein format	Scientific Literature
6.	ALA2175359	B	Inhibition of Trypanosoma brucei TbrPDEB1	Trypanosoma brucei	1		single protein format	Scientific Literature
7.	ALA2175364	B	Inhibition of Trypanosoma brucei His-tagged catalytic domains of TbrPDEB1 expressed in Escherichia coli BL21 assessed as conversion of cAMP to AMP after 20 mins by PDELight assay	Trypanosoma brucei	1		assay format	Scientific Literature
8.	ALA2175366	B	Inhibition of Trypanosoma brucei recombinant TbrPDEB1 expressed in Sf21 insect cells assessed as reduction of [3H]-cAMP hydrolysis by scintillation proximity assay	Trypanosoma brucei	31		cell-based format	Scientific Literature
9.	ALA2330147	B	Inhibition of Trypanosoma brucei full length PDEB1 expressed in insect SF21 cells using cAMP as substrate by scintillation proximity assay	Trypanosoma brucei	7		cell-based format	Scientific Literature
10.	ALA3361695	B	Inhibition of Trypanosoma brucei recombinant PDEB1 at 10 uM pre-incubated for 5 mins prior to substrate addition	Trypanosoma brucei	21		single protein format	Scientific Literature
11.	ALA3361696	B	Inhibition of Trypanosoma brucei recombinant PDEB1 pre-incubated for 5 mins prior to substrate addition	Trypanosoma brucei	10		single protein format	Scientific Literature
12.	ALA3769086	B	Inhibition of recombinant Trypanosoma brucei PDEB1 using [3H]-cAMP as substrate after 15 mins by scintillation proximity assay	Trypanosoma brucei	23		single protein format	Scientific Literature
13.	ALA4183750	B	Inhibition of full length Trypanosoma brucei PDEB1 expressed in Sf21 insect cells using cAMP as substrate by scintillation proximity assay	Trypanosoma brucei	25		cell-based format	Scientific Literature
14.	ALA4183753	B	Inhibition of PDEB1 in bloodstream form of Trypanosoma brucei Lister 427 assessed as increase in intracellular cAMP levels after 5 hrs by ELISA	Trypanosoma brucei	1		cell-based format	Scientific Literature
15.	ALA4183754	B	Inhibition of PDEB1 in bloodstream form of Trypanosoma brucei Lister 427 assessed as increase in intracellular cAMP levels at 0.3 uM after 5 hrs by ELISA	Trypanosoma brucei	1		cell-based format	Scientific Literature
16.	ALA4183755	B	Inhibition of PDEB1 in bloodstream form of Trypanosoma brucei Lister 427 assessed as increase in intracellular cAMP levels at 0.025 uM after 5 hrs by ELISA	Trypanosoma brucei	1		cell-based format	Scientific Literature
17.	ALA4350262	B	Inhibition of Trypanosoma brucei PDEB1 catalytic domain (565 to 918 residues) expressed in Escherichia coli BL21 incubated for 20 mins using cAMP as substrate by PDELight HTS cAMP phosphodiesterase Kit based luminometry	Trypanosoma brucei	28		assay format	Scientific Literature
18.	ALA4350844	B	Inhibition of Trypanosoma brucei PDEB1 catalytic domain using cAMP as substrate incubated for 20 mins by PDELight HTS cAMP phosphodiesterase Kit based luminometry	Trypanosoma brucei	25		single protein format	Scientific Literature
19.	ALA4350848	B	Binding affinity to Trypanosoma brucei PDEB1 assessed as dissociation constant treated for 60 secs measured for 300 secs by SPR assay	Trypanosoma brucei	1		single protein format	Scientific Literature
20.	ALA4350850	B	Binding affinity to Trypanosoma brucei PDEB1 assessed as dissociation constant treated for 320 secs measured for 900 secs by SPR assay	Trypanosoma brucei	1		single protein format	Scientific Literature