| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA2019640 | B | Inhibition of UBA3-mediated NEDD8 activation at 10 mM after 1.5 hrs by Western blot analysis | 2 | ALA2016531 | single protein format | Scientific Literature | ||
| 2. | ALA3880799 | B | Inhibition of recombinant human untagged NAEalpha/N-terminal His-tagged NAEbeta expressed in Bac-to-Bac expression system using FLAG-tagged NEDD8 as substrate after 90 mins in presence of GST-tagged Ubc12 by HTRF assay | Homo sapiens | 123 | ALA3880402 | assay format | Patent Bioactivity Data | |
| 3. | ALA4222565 | B | Inhibition of human recombinant His-tagged NAE1 expressed in baculovirus expression system after 90 mins in presence of FLAG-tagged NEDD8 by TR-FRET assay | Homo sapiens | 1 | ALA4219151 | assay format | Scientific Literature | |
| 4. | ALA4398580 | B | Inhibition of NEDD8-activating enzyme E1C (unknown origin) assessed as effect on NEDD8/UBE2M complex formation at 10 to 20 uM pre-incubated for 10 mins followed by Mg-ATP addition after 60 mins by SDS-PAGE analysis | Homo sapiens | 1 | ALA4396897 | single protein format | Scientific Literature | |
| 5. | ALA4398590 | B | Inhibition of NEDD8-activating enzyme E1C (unknown origin) assessed as effect on NEDD8/UBE2M complex formation up to 100 uM pre-incubated for 10 mins followed by Mg-ATP addition after 60 mins by SDS-PAGE analysis | Homo sapiens | 1 | ALA4396897 | single protein format | Scientific Literature |
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