# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA2026161 | B | Inhibition of nCDase in Moh.pAS cells using CerC12NBD as substrate after 1 hr by HPLC-FD analysis | 3 | ALA2021903 | cell-based format | Scientific Literature | ||
2. | ALA2026162 | B | Inhibition of nCDase using CerC12NBD as substrate after 1 hr by HPLC-FD analysis | 3 | ALA2021903 | single protein format | Scientific Literature | ||
3. | ALA2148526 | B | Activity of human recombinant neutral ceramidase assessed as hydrolysis at 20 uM after 60 mins by TLC-based fluorescence imaging | Homo sapiens | 4 | ALA2146367 | single protein format | Scientific Literature | |
4. | ALA2148646 | B | Activity of human recombinant neutral ceramidase after 60 mins by Lineweaver-Burk plot analysis | Homo sapiens | 4 | ALA2146367 | single protein format | Scientific Literature | |
5. | ALA2148648 | B | Activity of human recombinant neutral ceramidase assessed per ng of protein after 60 mins by Lineweaver-Burk plot analysis | Homo sapiens | 4 | ALA2146367 | single protein format | Scientific Literature | |
6. | ALA2157593 | B | Inhibition of recombinant human nCDase expressed in Sf9 cells using fluorescently-labeled acyl-NBD-C12-ceramide as substrate at 80 uM after 2 hrs by fluorescence assay | Homo sapiens | 15 | ALA2151021 | cell-based format | Scientific Literature | |
7. | ALA2157594 | B | Inhibition of recombinant human nCDase expressed in Sf9 cells using fluorescently-labeled acyl-NBD-C12-ceramide as substrate after 2 hrs by fluorescence assay | Homo sapiens | 2 | ALA2151021 | cell-based format | Scientific Literature | |
8. | ALA2157595 | B | Ratio of compound IC50 to B-13 IC50 for recombinant human nCDase expressed in Sf9 cells using fluorescently-labeled acyl-NBD-C12-ceramide as substrate after 2 hrs by fluorescence assay | Homo sapiens | 1 | ALA2151021 | cell-based format | Scientific Literature | |
9. | ALA2157596 | B | Activity of recombinant human nCDase expressed in Sf9 cells at 300 uM after 2 hrs by fluorescence assay | Homo sapiens | 3 | ALA2151021 | cell-based format | Scientific Literature | |
10. | ALA2339251 | B | Inhibition of human recombinant neutral ceramidase using Acyl-NBD-C12-cer as substrate at 80 uM after 2 hrs by fluorescence assay | Homo sapiens | 9 | ALA2331537 | single protein format | Scientific Literature | |
11. | ALA2339252 | B | Inhibition of human recombinant neutral ceramidase using Acyl-NBD-C12-cer as substrate after 2 hrs by fluorescence assay | Homo sapiens | 2 | ALA2331537 | single protein format | Scientific Literature | |
12. | ALA3381634 | B | Inhibition of NCDase (unknown origin) expressed in HEK293 cells using D-e-C12-NBD-Cer substrate up to 250 uM after 1 hr by by densitometric analysis | Homo sapiens | 1 | ALA3351606 | cell-based format | Scientific Literature | |
13. | ALA3381637 | B | Inhibition of NCDase (unknown origin) expressed in HEK293 cells using D-e-C12-NBD-Cer substrate at 30 uM after 1 hr by by densitometric analysis | Homo sapiens | 1 | ALA3351606 | cell-based format | Scientific Literature | |
14. | ALA3382952 | B | Inhibition of NCDase in human HeLa cells expressing ASAH2 assessed as increase in Sph level at 10 uM after 1 hr by LC-MS/MS analysis | Homo sapiens | 1 | ALA3351606 | cell-based format | Scientific Literature | |
15. | ALA3382953 | B | Inhibition of NCDase in human MCF7 cells expressing ASAH2 assessed as increase in Sph level at 10 uM after 1 hr by LC-MS/MS analysis | Homo sapiens | 1 | ALA3351606 | cell-based format | Scientific Literature | |
16. | ALA4816175 | B | Inhibition of human ASAH2 stably over expressed in human A-375 cell lysate using RBM14C24 fluorogenic substrate measured at 20 uM at pH 7.5 by fluorescence spectrophotometry | Homo sapiens | 17 | ALA4813974 | subcellular format | Scientific Literature | |
17. | ALA4816179 | B | Non-competitive inhibition of recombinant ASAH2 stably over expressed in human A-375 cell lysate using RBM14C24 as substrate | Homo sapiens | 4 | ALA4813974 | subcellular format | Scientific Literature | |
18. | ALA4816187 | B | Inhibition of human ASAH2 stably over expressed in human A-375 cell lysate using RBM14C24 fluorogenic substrate measured at 1:1 substrate to compound molar ratio at pH 7.5 by fluorescence spectrophotometry | Homo sapiens | 2 | ALA4813974 | subcellular format | Scientific Literature | |
19. | ALA4816188 | B | Inhibition of human ASAH2 stably over expressed in human A-375 cell lysate using RBM14C24 fluorogenic substrate measured at 5 uM at pH 7.5 by fluorescence spectrophotometry | Homo sapiens | 2 | ALA4813974 | subcellular format | Scientific Literature | |
20. | ALA4816189 | B | Inhibition of human ASAH2 stably over expressed in human A-375 cell lysate using RBM14C24 fluorogenic substrate measured at 20:1 substrate to compound molar ratio at pH 7.5 by fluorescence spectrophotometry | Homo sapiens | 2 | ALA4813974 | subcellular format | Scientific Literature |