| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA2033342 | B | Inhibition of Epac1 at 25 uM in presence of equal molar concentration of cAMP | 1 | single protein format | Scientific Literature | |||
| 2. | ALA2033347 | B | Inhibition of recombinant Epac1-mediated Rap1-GDP exchange activity at 25 uM by fluorescence analysis in presence of cAMP | 1 | single protein format | Scientific Literature | |||
| 3. | ALA2033350 | B | Inhibition of recombinant Epac1-mediated Akt phosphorylation at T308 expressed in 007-AM-stimulated HEK293 cells at 10 uM administered 5 mins prior to 007-AM by Western blot analysis | 2 | cell-based format | Scientific Literature | |||
| 4. | ALA2033351 | B | Inhibition of recombinant Epac1-mediated Akt phosphorylation at S473 expressed in 007-AM-stimulated HEK293 cells at 10 uM administered 5 mins prior to 007-AM by Western blot analysis | 2 | cell-based format | Scientific Literature | |||
| 5. | ALA2319308 | B | Inhibition FL-tagged full length human EPAC1 expressed in HEK293 cells assessed as inhibition of 007-AM-induced activation at 10 uM by FRET assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 6. | ALA2319313 | B | Inhibition of recombinant EPAC1 (unknown origin) assessed as inhibition of cAMP-mediated RAP1-GDP exchange activity at 25 uM by spectrofluorometric analysis | Homo sapiens | 3 | single protein format | Scientific Literature | ||
| 7. | ALA3215079 | F | PubChem BioAssay. qHTS for Agonist of cAMP-regulated guanine nucleotide exchange factor 3 (EPAC1): primary screen. (Class of assay: confirmatory) | 2,522 | assay format | PubChem BioAssays | |||
| 8. | ALA3214967 | F | PubChem BioAssay. qHTS for Antagonist of cAMP-regulated guanine nucleotide exchange factor 3 (EPAC1): primary screen. (Class of assay: confirmatory) | 12,729 | assay format | PubChem BioAssays | |||
| 9. | ALA3269757 | B | Inhibition of EPAC1 (unknown origin) assessed as cAMP-mediated GEF activity | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 10. | ALA3595149 | B | Antagonist activity at EPAC1 (unknown origin) assessed as cAMP-induced guanine nucleotide exchange factor activity | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 11. | ALA3595153 | B | Antagonist activity at human recombinant EPAC1 assessed as inhibition of Rap1b-bGDP exchange activity | Homo sapiens | 13 | single protein format | Scientific Literature | ||
| 12. | ALA3810791 | B | Inhibition of EPAC1 (unknown origin) assessed as reduction in cAMP-mediated guanine nucleotide exchange factor activity up to 100 uM in presence of C-terminal truncated Rap1B (1 to 167 residues)-BODIPY-GDP by microplate reader analysis | Homo sapiens | 4 | single protein format | Scientific Literature | ||
| 13. | ALA3810795 | B | Inhibition of recombinant C-terminal FLAG/HA-tagged EPAC1 (unknown origin) expressed in retrovirus infected human HeLaS3 cells assessed as reduction in cAMP-mediated guanine nucleotide exchange factor activity in presence of C-terminal truncated Rap1B (1 to 167 residues)-Mant-GDP by spectrofluorometric analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 14. | ALA4021027 | B | Antagonist activity at recombinant human full length EPAC1 assessed as inhibition of EPAC1-mediated Rap1B (1 to 167 residues)-BODIPY GDP nucleotide exchange activity by 8-NBD-cAMP competition assay | Homo sapiens | 31 | single protein format | Scientific Literature | ||
| 15. | ALA4136961 | B | Inhibition of recombinant human GST-tagged EPAC1 (149 to 881 residues) expressed in Escherichia coli Rosetta 2(DE3) assessed as reduction in guanine nucleotide exchange activity at 10 uM using BODIPY-labeled GDP loaded Rap1A by fluorescence assay relative to control | Homo sapiens | 12 | assay format | Scientific Literature | ||
| 16. | ALA4136962 | B | Inhibition of recombinant human GST-tagged EPAC1 (149 to 881 residues) expressed in Escherichia coli Rosetta 2(DE3) assessed as reduction in guanine nucleotide exchange activity at 50 uM using BODIPY-labeled GDP loaded Rap1A by fluorescence assay relative to control | Homo sapiens | 6 | assay format | Scientific Literature | ||
| 17. | ALA4136963 | B | Inhibition of recombinant human GST-tagged EPAC1 (149 to 881 residues) expressed in Escherichia coli Rosetta 2(DE3) assessed as reduction in guanine nucleotide exchange activity using BODIPY-labeled GDP loaded Rap1A by fluorescence assay | Homo sapiens | 4 | assay format | Scientific Literature | ||
| 18. | ALA4178330 | B | Inhibition of EPAC1 (unknown origin) | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 19. | ALA4178332 | B | Displacement of 8-NBD-cAMP from recombinant human EPAC1 by fluorescence assay | Homo sapiens | 2 | single protein format | Scientific Literature | ||
| 20. | ALA4178333 | B | Activation of EPAC1 DEP1 deletion mutant (unknown origin) expressed in mouse NIH-3T3-A14 cells assessed as increase in Rap1b- fluorescent nucleotide methylontraniloyl-GDP nucleotide exchange by fluorescence assay | Homo sapiens | 1 | cell-based format | Scientific Literature |
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