| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA2033343 | B | Inhibition of Epac2 at 25 uM in presence of equal molar concentration of cAMP | 1 | ALA2029310 | single protein format | Scientific Literature | ||
| 2. | ALA2033344 | B | Competitive inhibition of Epac2 using fluorescent nucleotide analog 8-NBD-cAMP by fluorescence analysis | 19 | ALA2029310 | single protein format | Scientific Literature | ||
| 3. | ALA2033345 | B | Competitive inhibition of Epac2 using fluorescent nucleotide analog 8-NBD-cAMP by fluorescence analysis relative to cAMP | 19 | ALA2029310 | single protein format | Scientific Literature | ||
| 4. | ALA2033346 | B | Inhibition of cAMP-mediated recombinant Epac2 GEF activity at 25 uM by fluorescence analysis in presence of cAMP | 2 | ALA2029310 | single protein format | Scientific Literature | ||
| 5. | ALA2033352 | B | Inhibition of recombinant Epac2-mediated Akt phosphorylation at T308 expressed in 007-AM-stimulated HEK293 cells at 10 uM administered 5 mins prior to 007-AM by Western blot analysis | 2 | ALA2029310 | cell-based format | Scientific Literature | ||
| 6. | ALA2033353 | B | Inhibition of recombinant Epac2-mediated Akt phosphorylation at S473 expressed in 007-AM-stimulated HEK293 cells at 10 uM administered 5 mins prior to 007-AM by Western blot analysis | 2 | ALA2029310 | cell-based format | Scientific Literature | ||
| 7. | ALA2319310 | B | Inhibition FL-tagged full length human EPAC2 expressed in HEK293 cells assessed as inhibition of 007-AM-induced activation at 10 uM by FRET assay | Homo sapiens | 1 | ALA2311500 | cell-based format | Scientific Literature | |
| 8. | ALA2319314 | B | Inhibition of recombinant EPAC2 (unknown origin) assessed as inhibition of cAMP-mediated guanine nucleotide exchange factor activity at 25 uM by spectrofluorometric analysis | Homo sapiens | 3 | ALA2311500 | single protein format | Scientific Literature | |
| 9. | ALA2319315 | B | Antagonist activity at recombinant EPAC2 (unknown origin) assessed 8-NBD-cAMP substrate fluorescence intensity by substrate competing assay | Homo sapiens | 34 | ALA2311500 | single protein format | Scientific Literature | |
| 10. | ALA2319316 | B | Ratio of cAMP IC50 to compound IC50 for recombinant EPAC2 (unknown origin) | Homo sapiens | 33 | ALA2311500 | single protein format | Scientific Literature | |
| 11. | ALA3215046 | F | PubChem BioAssay. qHTS for Antagonist of cAMP-regulated guanine nucleotide exchange factor 2 (EPAC2): primary screen. (Class of assay: confirmatory) | 9,563 | ALA1201862 | assay format | PubChem BioAssays | ||
| 12. | ALA3214906 | F | PubChem BioAssay. qHTS for Agonist of cAMP-regulated guanine nucleotide exchange factor 4 (EPAC2): primary screen. (Class of assay: confirmatory) | 1,697 | ALA1201862 | assay format | PubChem BioAssays | ||
| 13. | ALA3269755 | B | Displacement of 8-NBD-cAMP from EPAC2 (unknown origin) by fluorescence plate reader analysis | Homo sapiens | 4 | ALA3259785 | single protein format | Scientific Literature | |
| 14. | ALA3269756 | B | Inhibition of EPAC2 (unknown origin) | Homo sapiens | 3 | ALA3259785 | single protein format | Scientific Literature | |
| 15. | ALA3595079 | B | Displacement of 8-NBD-cAMP from EPAC2 (unknown origin) | Homo sapiens | 1 | ALA3593245 | single protein format | Scientific Literature | |
| 16. | ALA3595148 | B | Antagonist activity at EPAC2 (unknown origin) assessed as cAMP-induced guanine nucleotide exchange factor activity | Homo sapiens | 1 | ALA3593245 | single protein format | Scientific Literature | |
| 17. | ALA3810792 | B | Inhibition of 8-NBD-cAMP binding to EPAC2 (unknown origin) by microplate reader analysis | Homo sapiens | 31 | ALA3808331 | single protein format | Scientific Literature | |
| 18. | ALA3810793 | B | Inhibition of EPAC2 (unknown origin) assessed as reduction in cAMP-mediated guanine nucleotide exchange factor activity in presence of C-terminal truncated Rap1B (1 to 167 residues)-BODIPY-GDP by microplate reader analysis | Homo sapiens | 5 | ALA3808331 | single protein format | Scientific Literature | |
| 19. | ALA3810794 | B | Inhibition of recombinant C-terminal FLAG/HA-tagged EPAC2 (unknown origin) expressed in retrovirus infected human HeLaS3 cells assessed as reduction in cAMP-mediated guanine nucleotide exchange factor activity in presence of C-terminal truncated Rap1B (1 to 167 residues)-Mant-GDP by spectrofluorometric analysis | Homo sapiens | 1 | ALA3808331 | cell-based format | Scientific Literature | |
| 20. | ALA3810796 | B | Non-competitive inhibition of EPAC2 (unknown origin) assessed as reduction in cAMP-mediated guanine nucleotide exchange factor activity at 1 uM in presence of C-terminal truncated Rap1B (1 to 167 residues)-BODIPY-GDP | Homo sapiens | 1 | ALA3808331 | single protein format | Scientific Literature |
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