#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA2038205	B	Inhibition of Wistar/ST rat lung NAAA assessed as conversion of [14C]PEA to [14C]palmitic acid at 100 uM after 20 mins	Rattus norvegicus	8		single protein format	Scientific Literature
2.	ALA2038206	B	Inhibition of Wistar/ST rat lung NAAA assessed as conversion of [14C]PEA to [14C]palmitic acid after 20 mins	Rattus norvegicus	2		single protein format	Scientific Literature
3.	ALA2038207	B	Competitive inhibition of Wistar/ST rat lung NAAA using [14C]PEA as substrate at 5 uM by Line-Weaver Burk plot analysis	Rattus norvegicus	1		single protein format	Scientific Literature
4.	ALA2040243	B	Competitive inhibition of Wistar/ST rat lung NAAA using [14C]PEA as substrate at 10 uM by Line-Weaver Burk plot analysis	Rattus norvegicus	1		single protein format	Scientific Literature
5.	ALA2040244	B	Inhibition of Wistar/ST rat lung NAAA assessed as residual activity for conversion of [14C]PEA to [14C]palmitic acid at 100 uM after 20 mins relative to control	Rattus norvegicus	22		single protein format	Scientific Literature
6.	ALA2066781	B	Inhibition of rat recombinant NAAA expressed in HEK293 cells using heptadecenoylethanolamide as substrate after 30 mins by LC/MS analysis	Rattus norvegicus	17		cell-based format	Scientific Literature
7.	ALA1217267	B	Inhibition of rat recombinant NAAA expressed in HEK293 cells after 30 mins	Rattus norvegicus	25		cell-based format	Scientific Literature
8.	ALA1217268	B	Inhibition of rat recombinant NAAA expressed in HEK293 cells assessed as recovered initial enzyme activity at 10 uM after 12 hrs of dialysis	Rattus norvegicus	1		cell-based format	Scientific Literature
9.	ALA1217269	B	Non competitive inhibition of rat recombinant NAAA expressed in HEK293 cells at 100 nM by Michaelis-Menten equation analysis	Rattus norvegicus	1		cell-based format	Scientific Literature
10.	ALA1217270	B	Non competitive inhibition of rat recombinant NAAA expressed in HEK293 cells assessed per mg of protein at 100 nM by Michaelis-Menten equation analysis	Rattus norvegicus	1		cell-based format	Scientific Literature
11.	ALA1217271	B	Non competitive inhibition of rat recombinant NAAA expressed in HEK293 cells at 200 nM by Michaelis-Menten equation analysis	Rattus norvegicus	1		cell-based format	Scientific Literature
12.	ALA1217272	B	Non competitive inhibition of rat recombinant NAAA expressed in HEK293 cells assessed per mg of protein at 200 nM by Michaelis-Menten equation analysis	Rattus norvegicus	1		cell-based format	Scientific Literature
13.	ALA2421059	B	Inhibition of Sprague Dawley rat lung native NAAA enzyme using heptadecenoylethanolamide as substrate preincubated for 30 mins followed by substrate addition measured after 30 mins by UPLC-MS analysis	Rattus norvegicus	26		single protein format	Scientific Literature
14.	ALA2421060	B	Inhibition of recombinant rat lung NAAA enzyme using heptadecenoylethanolamide as substrate by LC/MS analysis	Rattus norvegicus	2		single protein format	Scientific Literature
15.	ALA3383170	B	Inhibition of NAAA in Sprague-Dawley rat lung assessed as inhibition of hydrolysis of 10-cis-heptadecenoylethanolamide by UPLC/MS method	Rattus norvegicus	38		single protein format	Scientific Literature
16.	ALA3888344	B	NAAA Assay: Recombinant NAAA or native rat lung NAAA was incubated at 37 °C. for 30 min in 0.2 ml of sodium hydrogen phosphate buffer (50 mM, pH 5.0) containing 0.1% Triton X-100, 3 mM dithiothreitol (DTT) and 50 mM heptadecenoylethanolamide as substrate. The reaction was terminated by the addition of 0.2 ml cold methanol containing 1 mmol of heptadecanoic acid (HDA, NuChek Prep, Elysian, Minn.). Samples were analyzed by LC/MS (liquid chromatography/mass spectrometry). Heptadecanoic acid was eluted on an XDB Eclipse C18 column isocratically at 2.2 ml/min for 1 min with a solvent mixture of 95% methanol and 5% water, both containing 0.25% acetic acid and 5 mM ammonium acetate. The column temperature was 50 °C. ESI was in the negative mode, capillary voltage was 4 kV, and fragmentor voltage was 100 V. N2 was used as drying gas at a flow rate of 13 liters/min and a temperature of 350 °C. Nebulizer pressure was set at 60 psi.		25		single protein format	BindingDB Database
17.	ALA3888531	B	Enzymatic Assay: Lysosomal NAAA protein preparation were obtained by homogenizing male Sprague-Dawley rat lungs (Charles River) in 20 mM Tris-HCl buffer pH 7.4 containing 0.32M sucrose. Samples were centrifuged at 800×g for 15 minutes at 4° C. Supernatants were then centrifuged at 12,000 g for 30 minutes at 4° C. Pellets were then resuspended in PBS pH 7.4 and subjected to a freeze/thaw cycle at −80° C. The suspension was finally centrifuged at 105,000×g for 1 hour at 4° C. The supernatant was then used in the enzymatic assay.		42		single protein format	BindingDB Database
18.	ALA4003207	B	Inhibition of rat NAAA expressed in HEK cells after 30 mins	Rattus norvegicus	1		cell-based format	Scientific Literature
19.	ALA4177791	B	Inhibition of rat NAAA expressed in HEK293 cells by LC/MS analysis	Rattus norvegicus	1		cell-based format	Scientific Literature
20.	ALA4177799	B	Inhibition of recombinant Sprague-Dawley rat NAAA expressed in HEK293 cells after 10 mins	Rattus norvegicus	2		cell-based format	Scientific Literature