# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA2050116 | B | Inhibition of human TMEM16A transfected in FRT cells assessed as iodide influx using YFP-F46L/H148Q/I152L halide sensor after 10 mins by spectrofluorometric analysis | Homo sapiens | 30 | ALA2046432 | cell-based format | Scientific Literature | |
2. | ALA3998787 | B | Activation of human TMEM16A expressed in FRT cells co-expressing YFP assessed as increase in iodide influx at 10 uM measured after 10 mins for 2 to 12 sec by fluorescence assay | Homo sapiens | 2 | ALA3997791 | cell-based format | Scientific Literature | |
3. | ALA3998788 | B | Inhibition of human TMEM16A expressed in FRT cells co-expressing YFP assessed as decrease in iodide influx at 10 uM measured after 10 mins for 2 to 12 sec in presence of ATP by fluorescence assay | Homo sapiens | 2 | ALA3997791 | cell-based format | Scientific Literature | |
4. | ALA4009591 | B | Inhibition of human TMEM16A expressed in FRT cells co-expressing YFP assessed as decrease in iodide influx at 10 uM measured after 10 mins in presence of ATP by fluorescence assay | Homo sapiens | 1 | ALA4007437 | cell-based format | Scientific Literature | |
5. | ALA4009592 | B | Activation of human TMEM16A expressed in FRT cells co-expressing YFP assessed as increase in iodide influx at 10 uM measured after 10 mins by fluorescence assay | Homo sapiens | 1 | ALA4007437 | cell-based format | Scientific Literature | |
6. | ALA4015867 | B | Inhibition of human TMEM16A expressed in FRT cells co-expressing iodide sensitive fluorescent protein YFP-H148Q/I152L/F46L assessed as reduction in ATP-induced Ca2+ activation-mediated chloride current by measuring decrease in iodide influx preincubated for 10 mins followed by ATP/iodide addition by fluorescence assay | Homo sapiens | 50 | ALA4014325 | cell-based format | Scientific Literature | |
7. | ALA4015868 | B | Inhibition of human TMEM16A expressed in FRT cells assessed as reduction of ATP-induced in chloride conductance preincubated for 5 mins followed by ATP addition by short-circuit current assay | Homo sapiens | 9 | ALA4014325 | cell-based format | Scientific Literature | |
8. | ALA4015870 | B | Inhibition of human TMEM16A expressed in FRT cells assessed as reduction of ATP-induced in chloride conductance preincubated for 20 mins followed by ATP addition by short-circuit current assay | Homo sapiens | 1 | ALA4014325 | cell-based format | Scientific Literature | |
9. | ALA4015873 | B | Inhibition of TMEM16A (unknown origin) expressed in Xenopus laevis oocytes at -60 mV holding potential by voltage-clamp assay | Homo sapiens | 1 | ALA4014325 | cell-based format | Scientific Literature | |
10. | ALA4015875 | B | Inhibition of human TMEM16A expressed in FRT cells assessed as reduction in Ca2+ activated chloride current at 250 nM at -60 mV holding potential by inside-out patch-clamp method | Homo sapiens | 1 | ALA4014325 | cell-based format | Scientific Literature | |
11. | ALA4015876 | B | Reversible inhibition of human TMEM16A expressed in FRT cells assessed as recovery of Ca2+ activated chloride current at 250 nM preincubated with cells followed by compound washout at -60 mV holding potential by inside-out patch-clamp method | Homo sapiens | 1 | ALA4014325 | cell-based format | Scientific Literature | |
12. | ALA4015877 | B | Inhibition of human TMEM16A expressed in FRT cells assessed as reduction in ionomycin activated chloride conductance at 1 uM preincubated for 5 mins followed by ionomycin addition by short-circuit current assay | Homo sapiens | 1 | ALA4014325 | cell-based format | Scientific Literature | |
13. | ALA4015879 | B | Inhibition of human TMEM16A expressed in FRT cells assessed as alteration of ATP stimulated cytoplasmic calcium elevation at 1 uM preincubated for 3 mins followed by ATP addition by Fluo-4 fluorescence assay | Homo sapiens | 1 | ALA4014325 | cell-based format | Scientific Literature | |
14. | ALA4015890 | B | Inhibition of human TMEM16A expressed in FRT cells assessed as reduction in carbachol activated chloride conductance at 1 uM preincubated for 5 mins followed by carbachol addition by short-circuit current assay | Homo sapiens | 1 | ALA4014325 | cell-based format | Scientific Literature | |
15. | ALA4310424 | B | Inhibition of YFP-fused ANO1 (unknown origin) expressed in FRT cells after 20 mins by fluorescence quenching method | Homo sapiens | 27 | ALA4308858 | cell-based format | Scientific Literature | |
16. | ALA4310426 | B | Inhibition of ANO1 (unknown origin) expressed in FRT cells assessed as reduction in ATP-induced chloride current after 20 mins by electrophysiological assay | Homo sapiens | 2 | ALA4308858 | cell-based format | Scientific Literature | |
17. | ALA4710535 | B | Inhibition of human ANO1 channel expressed in HEK293 cells at 10 uM incubated for 10 mins by FLIPR assay relative to control | Homo sapiens | 36 | ALA4706565 | cell-based format | Scientific Literature | |
18. | ALA4710536 | B | Inhibition of human ANO1 channel expressed in HEK293 cells incubated for 10 mins by FLIPR assay | Homo sapiens | 10 | ALA4706565 | cell-based format | Scientific Literature | |
19. | ALA4710548 | B | Induction of ANO1 degradation in human U251 cells at 10 uM preincubated with compound for 6 hrs followed by treatment with proteasome inhibitor, MG132 for 18 hrs by western blot analysis | Homo sapiens | 2 | ALA4706565 | cell-based format | Scientific Literature | |
20. | ALA4710558 | B | Induction of ANO1 degradation in human U251 cells at 10 uM incubated for 24 hrs by western blot analysis | Homo sapiens | 2 | ALA4706565 | cell-based format | Scientific Literature |