| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA2072790 | B | Inhibition of Keap1 interaction with Nrf2 in human THP1 cells assessed as increase in HO-1 mRNA expression at 100 uM from 3 to 6 hrs by qRT-PCR analysis | Homo sapiens | 3 | ALA2069269 | cell-based format | Scientific Literature | |
| 2. | ALA2072791 | B | Inhibition of Keap1 interaction with Nrf2 in human THP1 cells assessed as increase in Nrf2 protein expression at 100 uM after 3 hrs by Western blot analysis | Homo sapiens | 1 | ALA2069269 | cell-based format | Scientific Literature | |
| 3. | ALA2072792 | B | Inhibition of Keap1 interaction with Nrf2 in human THP1 cells assessed as increase in Nrf2 protein expression at 100 uM from 1 to 6 hrs by Western blot analysis | Homo sapiens | 1 | ALA2069269 | cell-based format | Scientific Literature | |
| 4. | ALA2072794 | B | Inhibition of Keap1 interaction with Nrf2 in human THP1 cells assessed as increase in HO-1 mRNA expression at 75 uM at 6 hrs by qRT-PCR analysis relative to control | Homo sapiens | 1 | ALA2069269 | cell-based format | Scientific Literature | |
| 5. | ALA2072795 | B | Inhibition of Keap1 interaction with Nrf2 in human THP1 cells assessed as increase in HO-1 mRNA expression at 100 uM from 2 to 8 hrs by qRT-PCR analysis | Homo sapiens | 2 | ALA2069269 | cell-based format | Scientific Literature | |
| 6. | ALA2072796 | B | Inhibition of Keap1 interaction with Nrf2 in human THP1 cells assessed as increase in HO-1 protein expression at 100 uM at 12 hrs by Western blot analysis | Homo sapiens | 1 | ALA2069269 | cell-based format | Scientific Literature | |
| 7. | ALA2072797 | B | Inhibition of Keap1 interaction with Nrf2 in human THP1 cells assessed as increase in HO-1 protein expression at 100 uM at 9 to 16 hrs by Western blot analysis | Homo sapiens | 1 | ALA2069269 | cell-based format | Scientific Literature | |
| 8. | ALA2072798 | B | Inhibition of Keap1 interaction with Nrf2 in human THP1 cells assessed as increase in HO-1 protein expression by Western blot analysis | Homo sapiens | 1 | ALA2069269 | cell-based format | Scientific Literature | |
| 9. | ALA2072799 | B | Inhibition of Keap1 interaction with Nrf2 in human THP1 cells assessed as increase in HO-1 protein expression at 37.5 uM by Western blot analysis | Homo sapiens | 1 | ALA2069269 | cell-based format | Scientific Literature | |
| 10. | ALA2114882 | F | PubChem BioAssay. Thermal shift assay to identify compound binding to Kelch domain of Keap1 protein Measured in Biochemical System Using RT-PCR - 2119-02_Inhibitor_Dose_CherryPick_Activity. (Class of assay: confirmatory) | Homo sapiens | 400 | ALA1201862 | biochemical format | PubChem BioAssays | |
| 11. | ALA2382890 | B | Binding affinity to Keap1 kelch domain (unknown origin) by competitive SPR assay | Homo sapiens | 8 | ALA2380257 | single protein format | Scientific Literature | |
| 12. | ALA2406872 | B | Binding affinity to human recombinant Kelch-DC domain of Keap1 (321 to 609) expressed in HEK293T cells using [2H]dithiothreitol at 1 mM by NMR-HSQC analysis | Homo sapiens | 2 | ALA2401631 | cell-based format | Scientific Literature | |
| 13. | ALA2406876 | B | Binding affinity to human recombinant Kelch-DC domain of Keap1 (321 to 609) expressed in HEK293T cells assessed as covalent adduct formation with cysteines at 1 mM after 15 hrs by 2D-FIDA assay | Homo sapiens | 2 | ALA2401631 | cell-based format | Scientific Literature | |
| 14. | ALA2406877 | B | Displacement of MR121-Nrf2 from human N-terminal His-tagged Kelch-DC domain of Keap1 (321 to 609) expressed in Escherichia coli BL21 (DE3) after 40 mins by 2D-FIDA assay | Homo sapiens | 3 | ALA2401631 | assay format | Scientific Literature | |
| 15. | ALA3135825 | B | Inhibition of FITC-9mer Nrf2 peptide amide/Keap1 Kelch domain (unknown origin) interaction after 30 mins by fluorescence polarization assay | Homo sapiens | 3 | ALA3132777 | single protein format | Scientific Literature | |
| 16. | ALA3214844 | B | PubChem BioAssay. Counterscreen to eliminate false positive from thermal shift assay Measured in Biochemical System Using Plate Reader - 2119-07_Inhibitor_Dose_CherryPick_Activity. (Class of assay: confirmatory) | 140 | ALA1201862 | biochemical format | PubChem BioAssays | ||
| 17. | ALA3214876 | B | PubChem BioAssay. Thermal shift assay to identify compound binding to Kelch domain of Keap1 protein Measured in Biochemical System Using RT-PCR - 2119-02_Inhibitor_Dose_DryPowder_Activity. (Class of assay: confirmatory) | 86 | ALA1201862 | biochemical format | PubChem BioAssays | ||
| 18. | ALA3239748 | B | Binding affinity to kelch domain of Keap1 (unknown origin) assessed as change in melting temperature after 15 mins by differential scanning fluorimetry assay | Homo sapiens | 7 | ALA3232949 | single protein format | Scientific Literature | |
| 19. | ALA3242827 | B | Binding affinity to biotinylated Keap1 DC domain (unknown origin) by biolayer interferometry assay | Homo sapiens | 3 | ALA3233002 | single protein format | Scientific Literature | |
| 20. | ALA3242828 | B | Binding affinity to recombinant human Keap1 Kelch domain (321 to 609) expressed in Escherichia coli BL21(DE3)pLysS cells after 30 mins by fluorescence anisotropy assay | Homo sapiens | 1 | ALA3233002 | cell-based format | Scientific Literature |
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