#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA2089532	B	Inhibition to His6-tagged Sos1 catalytic domain-mediated nucleotide exchange by fluorimetric assay		1	ALA2086330	single protein format	Scientific Literature
2.	ALA4137121	B	Activation of SOS1 in human HeLa cells assessed as effect on ERK1/2T202/Y204 phosphorylation levels by measuring ratio of total ERK1/2 level to pERK1/2T202/Y204 level at 50 uM after 30 mins by Western blot analysis relative to control	Homo sapiens	4	ALA4130625	cell-based format	Scientific Literature
3.	ALA4137122	B	Activation of SOS1 in human HeLa cells assessed as effect on ERK1/2T202/Y204 phosphorylation levels by measuring ratio of total ERK1/2 level to pERK1/2T202/Y204 level at 25 uM after 30 mins by Western blot analysis relative to control	Homo sapiens	4	ALA4130625	cell-based format	Scientific Literature
4.	ALA4137123	B	Activation of SOS1 in human HeLa cells assessed as effect on ERK1/2T202/Y204 phosphorylation levels by measuring ratio of total ERK1/2 level to pERK1/2T202/Y204 level at 12.5 uM after 30 mins by Western blot analysis relative to control	Homo sapiens	4	ALA4130625	cell-based format	Scientific Literature
5.	ALA4137124	B	Activation of SOS1 in human HeLa cells assessed as effect on ERK1/2T202/Y204 phosphorylation levels by measuring ratio of total ERK1/2 level to pERK1/2T202/Y204 level at 3.1 uM after 30 mins by Western blot analysis relative to control	Homo sapiens	4	ALA4130625	cell-based format	Scientific Literature
6.	ALA4137125	B	Activation of SOS1 in human HeLa cells assessed as effect on RAS-GTP levels by measuring ratio of total RAS level to RAS-GTP level at 50 uM after 30 mins by Western blot analysis relative to control	Homo sapiens	4	ALA4130625	cell-based format	Scientific Literature
7.	ALA4137126	B	Activation of SOS1 in human HeLa cells assessed as effect on RAS-GTP levels by measuring ratio of total RAS level to RAS-GTP level at 25 uM after 30 mins by Western blot analysis relative to control	Homo sapiens	4	ALA4130625	cell-based format	Scientific Literature
8.	ALA4137127	B	Activation of SOS1 in human HeLa cells assessed as effect on RAS-GTP levels by measuring ratio of total RAS level to RAS-GTP level at 6.3 uM after 30 mins by Western blot analysis relative to control	Homo sapiens	4	ALA4130625	cell-based format	Scientific Literature
9.	ALA4137128	B	Activation of SOS1 in human HeLa cells assessed as effect on RAS-GTP levels by measuring ratio of total RAS level to RAS-GTP level at 3.1 uM after 30 mins by Western blot analysis relative to control	Homo sapiens	4	ALA4130625	cell-based format	Scientific Literature
10.	ALA4137129	B	Activation of SOS1 in human HeLa cells assessed as effect on ERK1/2T202/Y204 phosphorylation levels by measuring ratio of total ERK1/2 level to pERK1/2T202/Y204 level at 6.3 uM after 30 mins by Western blot analysis relative to control	Homo sapiens	4	ALA4130625	cell-based format	Scientific Literature
11.	ALA4137130	B	Binding affinity to human recombinant N-terminal His6-tagged SOS1 (564 to 1049 residues) expressed in BL21-RIL Escherichia coli assessed as SOS1-RAS complex formation by measuring exchange of GTP to BODIPY-GDP at 100 uM measured every 3 secs for 30 mins by fluorescence polarization assay	Homo sapiens	41	ALA4130625	assay format	Scientific Literature
12.	ALA4137131	B	Binding affinity to human recombinant N-terminal His6-tagged SOS1 (564 to 1049 residues) expressed in BL21-RIL Escherichia coli assessed as SOS1-RAS complex formation by measuring exchange of GTP to BODIPY-GDP measured every 3 secs for 30 mins by fluorescence polarization assay	Homo sapiens	41	ALA4130625	assay format	Scientific Literature
13.	ALA4137132	B	Activation of SOS1 in human HeLa cells assessed as effect on RAS-GTP levels by measuring ratio of total RAS level to RAS-GTP level at 12.5 uM after 30 mins by Western blot analysis relative to control	Homo sapiens	4	ALA4130625	cell-based format	Scientific Literature
14.	ALA4137133	B	Activation of SOS1 in human HeLa cells assessed as increase in RAS-GTP levels after 30 mins by Western blot analysis	Homo sapiens	3	ALA4130625	cell-based format	Scientific Literature
15.	ALA4201750	B	Activation of human recombinant N-terminal His6-tagged SOS1 (564 to 1049 residues) expressed in BL21-RIL Escherichia coli assessed as SOS1-RAS complex formation by measuring exchange of GTP to BODIPY-GDP measured every 3 secs for 30 mins by nucleotide exchange assay	Homo sapiens	28	ALA4196202	assay format	Scientific Literature
16.	ALA4201751	B	Activation of human recombinant N-terminal His6-tagged SOS1 (564 to 1049 residues) expressed in BL21-RIL Escherichia coli assessed as SOS1-RAS complex formation by measuring exchange of GTP to BODIPY-GDP measured every 3 secs for 30 mins by nucleotide exchange assay relative to control	Homo sapiens	28	ALA4196202	assay format	Scientific Literature
17.	ALA4201752	B	Binding affinity to human recombinant N-terminal His6-tagged SOS1 (564 to 1049 residues) expressed in BL21-RIL Escherichia coli by fluorescent probe S1 based fluorescence polarization anisotrophy saturation binding assay	Homo sapiens	12	ALA4196202	assay format	Scientific Literature
18.	ALA4201753	B	Binding affinity to human recombinant N-terminal His6-tagged SOS1 (564 to 1049 residues) expressed in BL21-RIL Escherichia coli by fluorescent probe S3 based fluorescence polarization anisotrophy saturation binding assay	Homo sapiens	6	ALA4196202	assay format	Scientific Literature
19.	ALA4201754	B	Binding affinity to human recombinant N-terminal His6-tagged SOS1 (564 to 1049 residues) expressed in BL21-RIL Escherichia coli by fluorescent probe S2 based fluorescence polarization anisotrophy saturation binding assay	Homo sapiens	19	ALA4196202	assay format	Scientific Literature
20.	ALA4201755	B	Activation of SOS1 in human HeLa cells expressing wild type KRAS assessed as induction of ERK1/2 T202/Y204 phosphorylation incubated for 30 mins by In-cell Western assay	Homo sapiens	17	ALA4196202	cell-based format	Scientific Literature