| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA2089532 | B | Inhibition to His6-tagged Sos1 catalytic domain-mediated nucleotide exchange by fluorimetric assay | 1 | single protein format | Scientific Literature | |||
| 2. | ALA5251798 | B | Induction of SOS1 degradation in human NCI-H358 cells at 0.1 to 1 uM incubated for 24 hrs by immunoblot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 3. | ALA5251802 | B | Binding affinity to GST-tagged human SOS1 (564 to 1049 residues) expressed in Escherichia coli BL21 (DE3) cells assessed as binary complex formation by measuring dissociation constant incubated for 15 mins by fluorescence polarization-based displacement assay | Homo sapiens | 8 | cell-based format | Scientific Literature | ||
| 4. | ALA5251803 | B | Binding affinity to GST-tagged human SOS1 (564 to 1049 residues) expressed in Escherichia coli BL21 (DE3) cells assessed as ternary complex formation by measuring dissociation constant incubated for 15 mins in the presence of 6His tagged human VCB by fluorescence polarization-based displacement assay | Homo sapiens | 8 | cell-based format | Scientific Literature | ||
| 5. | ALA5251804 | B | Co-operativity factor, ratio of Kd for binding affinity to GST-tagged human SOS1 (564 to 1049 reisudes) expressed in Escherichia coli BL21 (DE3) cells assessed as binary complex formation to Kd for binding affinity to GST-tagged human SOS1 (564 to 1049 residues) expressed in Escherichia coli BL21 (DE3) cells assessed as ternary complex formation in the presence of 6His tagged human VCB | Homo sapiens | 7 | cell-based format | Scientific Literature | ||
| 6. | ALA5251864 | B | Induction of SOS1 degradation in human ASPC1 cells at 1 uM incubated for 6 hrs by quantitative proteomics analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 7. | ALA5251884 | F | Invivo induction of SOS1 degradation in human ASPC1 cells harboring KRAS G12D mutant xenografted BALB/c nude mouse assessed as decrease in SOS1 level at 50 mg/kg, ip administered twice daily for 3 weeks by Immunoblot analysis | Mus musculus | 1 | organism-based format | Scientific Literature | ||
| 8. | ALA5251886 | F | Invivo induction of SOS1 degradation in human ASPC1 cells harboring KRAS G12D mutant xenografted BALB/c nude mouse assessed as decrease in SOS1 level in tumor at 50 mg/kg, ip administered twice daily for 3 weeks by immunohistochemical analysis | Mus musculus | 1 | organism-based format | Scientific Literature | ||
| 9. | ALA5251892 | F | Invivo induction of SOS1 degradation in human SW620 cells harboring KRAS G12V mutant xenografted BALB/c nude mouse assessed as decrease in SOS1 level at 50 mg/kg, ip administered twice daily for 2 weeks by Immunoblot analysis | Mus musculus | 1 | organism-based format | Scientific Literature | ||
| 10. | ALA5251894 | F | Invivo induction of SOS1 degradation in human SW620 cells harboring KRAS G12V mutant xenografted BALB/c nude mouse assessed as decrease in SOS1 level in tumor at 50 mg/kg, ip administered twice daily for 2 weeks by immunohistochemistry analysis | Mus musculus | 1 | organism-based format | Scientific Literature | ||
| 11. | ALA4137121 | B | Activation of SOS1 in human HeLa cells assessed as effect on ERK1/2T202/Y204 phosphorylation levels by measuring ratio of total ERK1/2 level to pERK1/2T202/Y204 level at 50 uM after 30 mins by Western blot analysis relative to control | Homo sapiens | 4 | cell-based format | Scientific Literature | ||
| 12. | ALA4137122 | B | Activation of SOS1 in human HeLa cells assessed as effect on ERK1/2T202/Y204 phosphorylation levels by measuring ratio of total ERK1/2 level to pERK1/2T202/Y204 level at 25 uM after 30 mins by Western blot analysis relative to control | Homo sapiens | 4 | cell-based format | Scientific Literature | ||
| 13. | ALA4137123 | B | Activation of SOS1 in human HeLa cells assessed as effect on ERK1/2T202/Y204 phosphorylation levels by measuring ratio of total ERK1/2 level to pERK1/2T202/Y204 level at 12.5 uM after 30 mins by Western blot analysis relative to control | Homo sapiens | 4 | cell-based format | Scientific Literature | ||
| 14. | ALA4137124 | B | Activation of SOS1 in human HeLa cells assessed as effect on ERK1/2T202/Y204 phosphorylation levels by measuring ratio of total ERK1/2 level to pERK1/2T202/Y204 level at 3.1 uM after 30 mins by Western blot analysis relative to control | Homo sapiens | 4 | cell-based format | Scientific Literature | ||
| 15. | ALA4137125 | B | Activation of SOS1 in human HeLa cells assessed as effect on RAS-GTP levels by measuring ratio of total RAS level to RAS-GTP level at 50 uM after 30 mins by Western blot analysis relative to control | Homo sapiens | 4 | cell-based format | Scientific Literature | ||
| 16. | ALA4137126 | B | Activation of SOS1 in human HeLa cells assessed as effect on RAS-GTP levels by measuring ratio of total RAS level to RAS-GTP level at 25 uM after 30 mins by Western blot analysis relative to control | Homo sapiens | 4 | cell-based format | Scientific Literature | ||
| 17. | ALA4137127 | B | Activation of SOS1 in human HeLa cells assessed as effect on RAS-GTP levels by measuring ratio of total RAS level to RAS-GTP level at 6.3 uM after 30 mins by Western blot analysis relative to control | Homo sapiens | 4 | cell-based format | Scientific Literature | ||
| 18. | ALA4137128 | B | Activation of SOS1 in human HeLa cells assessed as effect on RAS-GTP levels by measuring ratio of total RAS level to RAS-GTP level at 3.1 uM after 30 mins by Western blot analysis relative to control | Homo sapiens | 4 | cell-based format | Scientific Literature | ||
| 19. | ALA4137129 | B | Activation of SOS1 in human HeLa cells assessed as effect on ERK1/2T202/Y204 phosphorylation levels by measuring ratio of total ERK1/2 level to pERK1/2T202/Y204 level at 6.3 uM after 30 mins by Western blot analysis relative to control | Homo sapiens | 4 | cell-based format | Scientific Literature | ||
| 20. | ALA4137130 | B | Binding affinity to human recombinant N-terminal His6-tagged SOS1 (564 to 1049 residues) expressed in BL21-RIL Escherichia coli assessed as SOS1-RAS complex formation by measuring exchange of GTP to BODIPY-GDP at 100 uM measured every 3 secs for 30 mins by fluorescence polarization assay | Homo sapiens | 41 | assay format | Scientific Literature |
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