| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA988882 | B | Inhibition of Cyclin T/CDK9 | 1 | ALA1142453 | single protein format | Scientific Literature | ||
| 2. | ALA3705381 | B | IMAP Assay: Cdk9/cyclinT1 is purchased from Millipore, cat #14-685. The final total protein concentration in the assay 4 nM. The 5TAMRA-cdk7tide peptide substrate, 5TAMRA-YSPTSPSYSPTSPSYSTPSPS--COOH, is purchased from Molecular Devices, cat#R7352. The final concentration of peptide substrate is 100 nM. The ATP substrate (Adenosine-5'-triphosphate) is purchased from Roche Diagnostics, cat#1140965. The final concentration of ATP substrate is 6 uM. IMAP (Immobilized Metal Assay for Phosphochemicals) Progressive Binding reagent is purchased from Molecular Devices, cat#R8139. Fluorescence polarization (FP) is used for detection. The 5TAMRA-cdk7tide peptide is phosphorylated by Cdk9/cyclinT1 kinase using the ATP substrate. The Phospho-5TAMRA-cdk7tide peptide substrate is bound to the IMAP Progressive Binding Reagent. | Homo sapiens | 348 | ALA3639283 | single protein format | BindingDB Database | |
| 3. | ALA4768773 | B | Inhibition of cyclin T1 (unknown origin) at 0.5 uM | Homo sapiens | 2 | ALA4765284 | single protein format | Scientific Literature | |
| 4. | ALA5118659 | B | Induction of HyT-mediated degradation of Cyclin T1 in human 22Rv1 cells at 0.3 to 3 uM incubated for 24 hrs by immunoblot analysis | Homo sapiens | 10 | ALA5113511 | cell-based format | Scientific Literature | |
| 5. | ALA5118660 | B | Induction of HyT-mediated degradation of Cyclin T1 in human 22Rv1 cells assessed as reduction in Cyclin T1 level at 3 uM incubated for 24 hrs by immunoblot analysis | Homo sapiens | 1 | ALA5113511 | cell-based format | Scientific Literature | |
| 6. | ALA5118663 | B | Induction of HyT-mediated degradation of Cyclin T1 in human 22Rv1 cells incubated for 24 hrs by immunoblot analysis | Homo sapiens | 1 | ALA5113511 | cell-based format | Scientific Literature | |
| 7. | ALA5118676 | B | Induction of Hyt-mediated degradation of cyclin T1 in human 22Rv1 cells assessed as downregulation of cyclin T1 level at 2 uM incubated for 24 hrs by DIA-based proteomics analysis | Homo sapiens | 1 | ALA5113511 | cell-based format | Scientific Literature | |
| 8. | ALA5118680 | B | Induction of Cyclin T1 degradation in human 22Rv1 cells assessed as cyclin T1 level at 3 uM incubated for 24 hrs by DIA-based proteomics analysis | Homo sapiens | 1 | ALA5113511 | cell-based format | Scientific Literature | |
| 9. | ALA5118686 | B | Induction of HA-tagged Cyclin T1 (unknown origin) polyubiquitination expressed in HEK293T cells co-transfected with Flag-tagged CDK9 and Myc ubiquitin assessed as increase in K48-linkage poly-ubiqutinated Cyclin T1 level at 1 to 2 uM incubated for 20 hrs by immunoprecipitation analysis | Homo sapiens | 1 | ALA5113511 | cell-based format | Scientific Literature | |
| 10. | ALA5118688 | B | Induction of HA-tagged Cyclin T1 (unknown origin) polyubiquitination expressed in HEK293T cells co-transfected with Flag-tagged CDK9 and Myc ubiquitin assessed as increase in K48-linkage poly-ubiqutinated Cyclin T1 level at 1 to 2 uM incubated for 20 hrs by western blot analysis | Homo sapiens | 1 | ALA5113511 | cell-based format | Scientific Literature | |
| 11. | ALA5118690 | B | Induction of HyT-mediated degradation of Cyclin T1 in human 22Rv1 cells at 0.3 to 3 uM incubated for 18 hrs in presence of proteasome inhibitor, MG132 by immunoblot analysis | Homo sapiens | 1 | ALA5113511 | cell-based format | Scientific Literature | |
| 12. | ALA5118693 | B | Induction of HyT-mediated degradation of Cyclin T1 in human 22Rv1 cells at 3 uM measured for 36 hrs by DIA-based proteomics analysis | Homo sapiens | 2 | ALA5113511 | cell-based format | Scientific Literature | |
| 13. | ALA5118695 | B | Induction of HyT-mediated degradation of Cyclin T1 in human 22Rv1 cells at 3 uM measured after 5 to 18 hrs by immunoblot analysis | Homo sapiens | 1 | ALA5113511 | cell-based format | Scientific Literature | |
| 14. | ALA5118697 | B | Induction of HyT-mediated degradation of Cyclin T1 in human 22Rv1 cells assessed as reduction in Cyclin T1 protein level at 3 uM measured after 24 to 48 hrs by immunoblot analysis | Homo sapiens | 1 | ALA5113511 | cell-based format | Scientific Literature | |
| 15. | ALA5118699 | B | Induction of degradation of Cyclin T1 in human 22Rv1 cells at 2 uM measured after 0 to 24 hrs by immunoblot analysis | Homo sapiens | 1 | ALA5113511 | cell-based format | Scientific Literature | |
| 16. | ALA5118712 | B | Induction of HyT-mediated degradation of Cyclin T1 in human 22Rv1 cells at 3 uM incubated for 24 hrs by immunoblot analysis relative to control | Homo sapiens | 1 | ALA5113511 | cell-based format | Scientific Literature | |
| 17. | ALA5118714 | B | Induction of degradation of Cyclin T1 in human 22Rv1 cells at 3 uM measured for 36 hrs by DIA-based proteomics analysis | Homo sapiens | 1 | ALA5113511 | cell-based format | Scientific Literature |
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