#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA1614105	F	PUBCHEM_BIOASSAY: AlphaScreen confirmatory assay for validation of inhibitors of SUMOylation. (Class of assay: confirmatory) [Related pubchem assays (depositor defined):AID2006, AID2011, AID2069, AID2614, AID2658]	Homo sapiens	327	ALA1201862	assay format	PubChem BioAssays
2.	ALA1614290	B	PUBCHEM_BIOASSAY: uHTS HTRF assay for identification of inhibitors of SUMOylation. (Class of assay: confirmatory) [Related pubchem assays (depositor defined):AID2011, AID2018, AID2069, AID2614, AID2658]	Homo sapiens	244	ALA1201862	protein complex format	PubChem BioAssays
3.	ALA2019532	B	Inhibition of human recombinant SAE1/2 assessed as inhibition of SUMO1-UbcH9 complex formation at 0.001 to 10 uM after 1.5 hrs by Western blot analysis	Homo sapiens	22	ALA2016531	protein complex format	Scientific Literature
4.	ALA2423585	B	Inhibition of SUMO E1 (unknown origin)-biotinylated SUMO-1 thioester complex formation at 10 uM after 20 mins by SDS-PAGE analysis in presence of ATP	Homo sapiens	2	ALA2417363	protein complex format	Scientific Literature
5.	ALA2423588	B	Inhibition of SUMO E1 (unknown origin)-mediated RanGAP1 sumoylation at < 30 uM by immunoblotting analysis	Homo sapiens	1	ALA2417363	protein complex format	Scientific Literature
6.	ALA2423589	B	Inhibition of SUMO E1 (unknown origin)-mediated RanGAP1 sumoylation at 100 uM by immunoblotting analysis	Homo sapiens	6	ALA2417363	protein complex format	Scientific Literature
7.	ALA2423590	B	Inhibition of SUMO E1 (unknown origin)-mediated RanGAP1 sumoylation by immunoblotting analysis	Homo sapiens	6	ALA2417363	protein complex format	Scientific Literature
8.	ALA2423591	B	Inhibition of SUMO E1 (unknown origin) assessed as SUMO E1-SUMO thioester bond formation	Homo sapiens	3	ALA2417363	protein complex format	Scientific Literature
9.	ALA3705806	B	Biochemical Assay: Through the Molecular Library Probe Production Center Network (MLPCN), at least 300,000 compounds were screened using a TR-FRET method, an ALPHASCREEN method or both, and tested for their ability to inhibit SUMOylation of a target protein via SUMO E1 or SUMO E2. The assays were based on SUMOylation of the target protein RanGAP1, which is a protein that is efficiently SUMOylated with only the SUMO E1 and E2 enzymes, and does not use E3 ligases. A fluorescence resonance energy transfer (FRET) assay was the primary assay followed by a chemoluminescence-based secondary assay using ALPHA screen to eliminate false positive hits. Then, the hits were screened by a poly-ubiquitination assay using ubiquitin, ubiquitin E1, Ubc5 and Apc11 to eliminate inhibitors not specific to SUMOylation. The screening identified a potent family of SUMOylation inhibitors based on a tricyclic scaffold.	Homo sapiens	13	ALA3638816	protein complex format	BindingDB Database
10.	ALA4143721	B	Inhibition of SAE (unknown origin) assessed as decrease in SAE-mediated Ubc9-SUMO conjugation levels up to 10 uM preincubated for 10 mins followed by ATP and Mg2+ addition measured after 60 mins by Western blot analysis	Homo sapiens	1	ALA4138244	protein complex format	Scientific Literature
11.	ALA4143750	B	Inhibition of SAE-mediated Ubc9-SUMO conjugation in human Caco2 cells up to 10 uM preincubated for 10 mins followed by ATP and Mg2+ addition measured after 60 mins by Western blot analysis	Homo sapiens	1	ALA4138244	cell-based format	Scientific Literature
12.	ALA4184766	B	Induction of SUMO-E1/E2 (unknown origin) mediated sumoylation of His-RanGap-1 after 30 mins by HTRF assay relative to control	Homo sapiens	11	ALA4184102	protein complex format	Scientific Literature
13.	ALA4185516	B	Inhibition of SAE (unknown origin) assessed as decrease in formation of E2-UBL thioester reaction product	Homo sapiens	1	ALA4184146	protein complex format	Scientific Literature
14.	ALA4271845	B	Inhibition of SAE in human AGS cells assessed as reduction in Uba9-SUMO conjugation level at 2 to 10 uM after 24 hrs by Western blot analysis	Homo sapiens	1	ALA4270526	cell-based format	Scientific Literature
15.	ALA4482791	B	Inhibition of SAE (unknown origin) using recombinant His-tagged human SUMO1 measured after 2 hrs by HTRF assay	Homo sapiens	24	ALA4482323	protein complex format	Patent Bioactivity Data
16.	ALA4834988	B	Inhibition of recombinant SAE (unknown origin) assessed as reduction in transfer of SUMO1 to UBC9 using SUMO1 as a substrate in presence of ATP at Km concentration by HTRF assay	Homo sapiens	60	ALA4834402	protein complex format	Scientific Literature
17.	ALA4834991	B	Inhibition of SAE (unknown origin) using SUMO1 as substrate incubated for 60 mins in presence of ATP and PPi by pyrophosphate exchange assay	Homo sapiens	2	ALA4834402	protein complex format	Scientific Literature
18.	ALA4834992	B	Inhibition of SAE (unknown origin) using SUMO2 as substrate incubated for 60 mins in presence of ATP and PPi by pyrophosphate exchange assay	Homo sapiens	2	ALA4834402	protein complex format	Scientific Literature
19.	ALA4834995	B	Inhibition of SAE in human HCT116 cells assessed as reduction in conjugation of SUMO proteins to UBC9 by Western blot analysis	Homo sapiens	2	ALA4834402	cell-based format	Scientific Literature
20.	ALA4834998	B	Inhibition of SAE in human HCT116 cells assessed as reduction in redistribution of SUMO2/3 proteins from nucleus to cytosol incubated for 4 hrs by immunofluorescence assay	Homo sapiens	59	ALA4834402	cell-based format	Scientific Literature