#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA750089	F	Compound was evaluated for agonist activity against activated human recombinant Nicotinic acetylcholine receptor alpha2-beta4 in Xenopus Oocytes	Homo sapiens	1		cell-based format	Scientific Literature
2.	ALA750090	F	Compound was evaluated for agonist activity against activated human recombinant Nicotinic acetylcholine receptor alpha2-beta4 in Xenopus Oocytes	Homo sapiens	1		cell-based format	Scientific Literature
3.	ALA752377	B	Binding affinity against nicotinic acetylcholine receptor alpha2-beta4 using [3H]epibatidine as radioligand expressed in HEK293 cells or tsA cells		3		cell-based format	Scientific Literature
4.	ALA830024	B	Effective concentration against Nicotinic acetylcholine receptor alpha2-beta4	Homo sapiens	2		protein complex format	Scientific Literature
5.	ALA829397	B	Inhibitory concentration against Nicotinic acetylcholine receptor alpha2-beta4	Homo sapiens	4		protein complex format	Scientific Literature
6.	ALA827552	B	Inhibitory concentration against Nicotinic acetylcholine receptor alpha2-beta4; Range is 0.3-1.5 nM	Homo sapiens	1		protein complex format	Scientific Literature
7.	ALA828589	B	Inhibitory concentration against Nicotinic acetylcholine receptor alpha2-beta4; Range is 0.3-1.5 nM	Homo sapiens	1		protein complex format	Scientific Literature
8.	ALA826909	B	Maximal response against Nicotinic acetylcholine receptor	Homo sapiens	1		protein complex format	Scientific Literature
9.	ALA826911	B	Maximal response against Nicotinic acetylcholine receptor alpha2-beta4	Homo sapiens	1		protein complex format	Scientific Literature
10.	ALA826912	B	Maximal response against Nicotinic acetylcholine receptor alpha2-beta4		6		protein complex format	Scientific Literature
11.	ALA827781	B	Maximal response against Nicotinic acetylcholine receptor alpha2-beta4 expressed in HEK293 cells	Homo sapiens	2		cell-based format	Scientific Literature
12.	ALA2209675	B	Displacement of [3H]epibatidine from alpha2beta4 nAChR at 10 uM after 4 hrs by liquid scintillation counting analysis		1		protein complex format	Scientific Literature
13.	ALA2210093	B	Displacement of [3H]epibatidine from alpha2beta4 nAChR after 4 hrs by liquid scintillation counting analysis		2		protein complex format	Scientific Literature
14.	ALA3888283	B	[3H]-Epibatidine Radioligand Binding Assay: Briefly, cultured cells at >80% confluence were removed from their flasks (80 cm^2) with a disposable cell scraper and placed in 10 mL of 50 mM Tris.HCl buffer (pH 7.4, 4 °C.). The cell suspension was centrifuged at 10,000×g for 5 min and the pellet was collected. The cell pellet was then homogenized in 10 mL buffer with a polytron homogenizer and centrifuged at 36,000 g for 10 min at 4 °C. The membrane pellet was resuspended in fresh buffer, and aliquots of the membrane preparation were used for binding assays. The concentration of [3H]-epibatidine used was ~500 pM for competition binding assays. Nonspecific binding was assessed in parallel incubations in the presence of 300 μM nicotine. Bound and free ligands were separated by vacuum filtration through Whatman GF/C filters treated with 0.5% polyethylenimine. The filter-retained radioactivity was measured by liquid scintillation counting. Specific binding was defined as the difference between total binding and nonspecific binding. Data from competition binding assays were analyzed using Prism 5 (GraphPad Software, San Diego, Calif.). The Kd values for [3H]-epibatidine used for calculating Ki values of nAChR subtypes were 0.02 nM for α2β2, 0.08 nM for α2β4, 0.03 nM for α3β2, 0.3 nM for α3β4, 0.04 nM for α4β2, 0.09 nM for α4β4, 1.8 nM for α7 and 0.05 for rat forebrain.		11		assay format	BindingDB Database
15.	ALA4811717	B	Inhibition of human alpha2beta4 nAChR expressed in Xenopus laevis oocytes assessed as inhibition of acetylcholine-induced current response by two-electrode voltage-clamp method	Homo sapiens	2		cell-based format	Scientific Literature
16.	ALA4811754	B	Inhibition of human alpha2beta4 nAChR expressed in Xenopus laevis oocytes assessed as inhibition of acetylcholine-induced current response at 10 uM by two-electrode voltage-clamp method relative to control	Homo sapiens	2		cell-based format	Scientific Literature
17.	ALA5034634	B	Inhibition of human alpha2beta4 nAChR expressed in Xenopus laevis oocytes assessed as inhibition of acetylcholine-induced current response by two-electrode voltage-clamp method	Homo sapiens	2		cell-based format	Scientific Literature
18.	ALA5215851	B	Inhibition of human alpha2beta4 nAChR expressed in xenopus oocytes assessed as inhibition of Ach- induced response at -70 mV holding potential and measured for 1 to 5 days in presence of Ach stimulation by voltage-clamp based electrophysiological method	Homo sapiens	1		cell-based format	Scientific Literature