# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA3807382 | Binding | Binding affinity to human recombinant N-terminal AVI-tagged phosphorylated AMPK alpha1beta1gamma1 expressed in Escherichia coli BL21 cells after 1 to 10 mins by biolayer interferometry | Homo sapiens | 1 | ALA3804759 | cell-based format | Scientific Literature | |
2. | ALA920467 | Binding | Inhibition of AMPK at 10 uM relative to control | Homo sapiens | 2 | ALA1149250 | protein complex format | Scientific Literature | |
3. | ALA1102598 | Binding | Inhibition of AMPK alpha-1/beta-1/gamma-1 at 10 uM | 2 | ALA1154277 | protein complex format | Scientific Literature | ||
4. | ALA1111289 | Binding | Inhibition of AMPK alpha-1/beta-1/gamma-1 at 5 uM | 1 | ALA1154316 | protein complex format | Scientific Literature | ||
5. | ALA1110414 | Binding | Inhibition of AMPK alpha-1/beta-1/gamma-1 at 100 nM relative to control | 1 | ALA1154334 | protein complex format | Scientific Literature | ||
6. | ALA1243992 | Binding | Inhibition of AMPK alpha-1/beta-1/gamma-1 at 1 uM after 60 mins by FRET assay in presence of 10 uM ATP | 5 | ALA1240340 | protein complex format | Scientific Literature | ||
7. | ALA1251633 | Binding | Inhibition of AMPK A1/B1/G1 at 1 uM in presence of 10 uM ATP by Select Screen kinase assay relative to untreated control | 3 | ALA1250448 | protein complex format | Scientific Literature | ||
8. | ALA4350305 | Binding | Activation of human AMPK alpha1/beta1/gamma1 expressed in baculovirus/Sf9 cells using SAMS peptide as substrate incubated for 15 mins by in presence of 33P-ATP by liquid scintillation counting method | Homo sapiens | 1 | ALA4346753 | cell-based format | Scientific Literature | |
9. | ALA4350309 | Binding | Activation of C-terminal His tagged human recombinant full length AMPK alpha1/beta1/gamma1 expressed in baculovirus infected Sf9 cells using SAMS peptide as substrate at 100 uM incubated for 60 mins by ADP-Glo luminescence assay relative to control | Homo sapiens | 16 | ALA4346753 | cell-based format | Scientific Literature | |
10. | ALA4350313 | Binding | Activation of C-terminal His tagged human recombinant full length AMPK alpha1/beta1/gamma1 expressed in baculovirus infected Sf9 cells using SAMS peptide as substrate incubated for 60 mins by ADP-Glo luminescence assay | Homo sapiens | 5 | ALA4346753 | cell-based format | Scientific Literature | |
11. | ALA4350322 | Binding | Activation of C-terminal His tagged human recombinant full length AMPK alpha1/beta1/gamma1 expressed in baculovirus infected Sf9 cells assessed as enzyme activation using SAMS peptide as substrate at 100 uM incubated for 60 mins by ADP-Glo luminescence assay relative to control | Homo sapiens | 4 | ALA4346753 | cell-based format | Scientific Literature | |
12. | ALA3394985 | Binding | Activation of human AMPK alpha1beta1gamma1 | Homo sapiens | 10 | ALA3393052 | protein complex format | Scientific Literature | |
13. | ALA3395069 | Binding | Activation of human AMPK alpha1beta1gamma1 relative to AMP | Homo sapiens | 10 | ALA3393052 | protein complex format | Scientific Literature | |
14. | ALA3395071 | Binding | Activation of human AMPK alpha1beta1gamma1 using [gamma-33P]ATP as substrate after 2 hrs by scintillation proximity assay | Homo sapiens | 7 | ALA3393052 | protein complex format | Scientific Literature | |
15. | ALA3854699 | Binding | Activation of recombinant human AMPK alpha1/beta1/gamma1 using Cy5-labelled SAMS as substrate assessed as protection from Thr172 residue dephosphorylation at 40 uM preincubated for 15 mins followed by incubation with PP2a for 60 mins measured 60 mins post okadaic acid/Cy5-labelled SAMS and ATP addition by TR-FRET assay relative to control | Homo sapiens | 1 | ALA3853317 | protein complex format | Scientific Literature | |
16. | ALA3854709 | Binding | Activation of recombinant human AMPK alpha1/beta1/gamma1 using Cy5-labelled SAMS as substrate assessed as protection from Thr172 residue dephosphorylation preincubated for 15 mins followed by incubation with PP2a for 60 mins measured 60 mins post okadaic acid/Cy5-labelled SAMS and ATP addition by TR-FRET assay | Homo sapiens | 7 | ALA3853317 | protein complex format | Scientific Literature | |
17. | ALA3854710 | Binding | Activation of recombinant human AMPK alpha1/beta1/gamma1 using Cy5-labelled SAMS as substrate assessed as protection from Thr172 residue dephosphorylation preincubated for 15 mins followed by incubation with PP2a for 60 mins measured 60 mins post okadaic acid/Cy5-labelled SAMS and ATP addition by TR-FRET assay relative to AMP | Homo sapiens | 8 | ALA3853317 | protein complex format | Scientific Literature | |
18. | ALA3854711 | Binding | Binding affinity to recombinant human AMPK alpha1/beta1/gamma1 by SPR binding assay | Homo sapiens | 7 | ALA3853317 | protein complex format | Scientific Literature | |
19. | ALA3854717 | Binding | Binding affinity to recombinant human AMPK alpha1/beta1/gamma1 assessed as association constant | Homo sapiens | 1 | ALA3853317 | protein complex format | Scientific Literature | |
20. | ALA3854718 | Binding | Binding affinity to recombinant human AMPK alpha1/beta1/gamma1 assessed as dissociation constant | Homo sapiens | 1 | ALA3853317 | protein complex format | Scientific Literature |