| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA710955 | B | Inhibition of ERK-phosphorylation by Raf-MEK-ERK in coupled ELISA | Homo sapiens | 14 | ALA1133366 | protein format | Scientific Literature | |
| 2. | ALA710956 | B | Inhibition of ERK phosphorylation in coupled Raf-MEK-ERK ELISA (Weakly active) | Homo sapiens | 1 | ALA1133366 | protein format | Scientific Literature | |
| 3. | ALA769165 | B | Inhibition of kinase activity of Raf/MEK/ERK kinase cascade in ELISA | Homo sapiens | 10 | ALA1135814 | protein format | Scientific Literature | |
| 4. | ALA772796 | B | Inhibition of kinase activity of Raf/MEK/ERK kinase cascade in ELISA (Not tested) | Homo sapiens | 1 | ALA1135814 | protein format | Scientific Literature | |
| 5. | ALA833860 | F | Inhibition of EGF-stimulated Elk1-luciferase reporter assay in HeLa cells | Homo sapiens | 11 | ALA1137122 | cell-based format | Scientific Literature | |
| 6. | ALA877427 | F | Inhibitory activity in EGF-stimulated Elk1-luciferase reporter assay in HeLa cells at 20 uM | Homo sapiens | 2 | ALA1137122 | cell-based format | Scientific Literature | |
| 7. | ALA825366 | F | Inhibitory activity in EGF-stimulated Elk1-luciferase reporter assay in HeLa cells at 15 uM | Homo sapiens | 1 | ALA1137122 | cell-based format | Scientific Literature | |
| 8. | ALA825367 | F | Inhibitory activity in EGF-stimulated Elk1-luciferase reporter assay in HeLa cells at 20 uM | Homo sapiens | 3 | ALA1137122 | cell-based format | Scientific Literature | |
| 9. | ALA825368 | F | Inhibitory activity in EGF-stimulated Elk1-luciferase reporter assay in HeLa cells at 30 uM | Homo sapiens | 1 | ALA1137122 | cell-based format | Scientific Literature | |
| 10. | ALA825369 | F | Inhibitory activity in EGF-stimulated Elk1-luciferase reporter assay in HeLa cells at 60 uM | Homo sapiens | 1 | ALA1137122 | cell-based format | Scientific Literature | |
| 11. | ALA884485 | F | Inhibitory activity against Raf/MEK | Homo sapiens | 1 | ALA1140394 | assay format | Scientific Literature | |
| 12. | ALA908992 | B | Inhibition of Raf1-MEK1 | Homo sapiens | 1 | ALA1139689 | protein format | Scientific Literature | |
| 13. | ALA3111853 | B | Inhibition of human RAF1/MEK1 at 1 uM | Homo sapiens | 1 | ALA3108740 | protein format | Scientific Literature | |
| 14. | ALA3292678 | B | Inhibition of RAF-1/MEK1 (unknown origin) assessed as at 10 uM after 5 mins by fluorescence assay relative to control | Homo sapiens | 10 | ALA3286227 | protein format | Scientific Literature | |
| 15. | ALA3887822 | B | Cascade Assay: A BRAF-MEK-ERK cascade assay is used to evaluate the effects of these compounds as inhibitors of the MAP kinase pathway. An enzymatic cascade assay is set up using recombinant human activated BRAF (V599E) kinase (Cat No. 14-557), human full length MEK1 kinase (Cat No. 14-706) and human full length active MAP Kinase 2/ERK2 (Cat No. 14-536) enzymes procured from Upstate. TR-FRET (Time resolved fluorescence resonance energy transfer) detection technology is used for the read out. The assay buffer solution contains 50 mM Tris pH 7.5, 10 mM MgCl2, 1 mM DTT, 0.01% Tween 20, 0.1 nM activated BRAF, 2 nM inactive MEK1, 10 nM inactive ERK2, 1 mM ATP and 500 nM long chain biotin-peptide substrate (LCB-FFKNIVTPRTPPP) in a 384 well format. The kinase reaction is stopped after 90 minutes with 10 mM EDTA and Lance detection mix (2 nM Eu-labeled phospho-serine/threonine antibody (Cat. No. AD0176-Perkin Elmer), 20 nM SA-APC (Cat No. CR130-100-Perkin Elmer) is added. | Homo sapiens | 99 | ALA3886462 | protein format | BindingDB Database | |
| 16. | ALA4835224 | B | Inhibition of RAF-1/MEK1 kinase (unknown origin) at 10 uM | Homo sapiens | 1 | ALA4834410 | protein format | Scientific Literature |
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