# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA2114802 | F | PubChem BioAssay. Fluorescence polarization-based biochemical high throughput dose response screening assay to identify inhibitors of ADP-ribosylation factor GTPase activating protein 1 (ARFGAP1). (Class of assay: confirmatory) | Rattus norvegicus | 91 | assay format | PubChem BioAssays | ||
2. | ALA3630622 | B | Inhibition of poly-histidine tagged full-length rat ARFGAP1 expressed in Sf9 cells assessed as remaining enzyme activity at 20 uM using purified WT ARF1 pre-loaded with [gamm-32P]-labeled GTP incubated for 5 mins before ARF1-GTP addition followed by 25 mins incubation by scintillation counting method | Rattus norvegicus | 42 | cell-based format | Scientific Literature | ||
3. | ALA3630623 | B | Inhibition of poly-histidine tagged full-length rat ARFGAP1 expressed in Sf9 cells assessed as remaining enzyme activity at 10 uM using purified WT ARF1 pre-loaded with [gamm-32P]-labeled GTP incubated for 5 mins before ARF1-GTP addition followed by 25 mins incubation by scintillation counting method | Rattus norvegicus | 42 | cell-based format | Scientific Literature |