# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA2114713 | F | PubChem BioAssay. qHTS Assay for Activators of ClpP. (Class of assay: confirmatory) | Bacillus subtilis | 20,646 | ALA1201862 | assay format | PubChem BioAssays | |
2. | ALA4136373 | B | Activation of Bacillus subtilis ClpP expressed in Escherichia coli BL21(DE3) using Abz-DFAPKMALVPYNO2 as substrate pretreated for 15 mins followed by substrate addition measured every 30 mins for 2 hrs by fluorescence assay | Bacillus subtilis | 3 | ALA4130565 | assay format | Scientific Literature | |
3. | ALA4136375 | B | Stabilization of Bacillus subtilis ClpP assessed as change in melting temperature at 50 uM after 30 mins by SYPRO orange protein gel staining based FRET analysis | Bacillus subtilis | 3 | ALA4130565 | single protein format | Scientific Literature | |
4. | ALA4477468 | B | Activation of Bacillus subtilis ClpP expressed in Escherichia coli BL21 (DE3) using beta casein as substrate at 50 uM preincubated for 15 mins followed by substrate addition measured every 30 mins by SDS-PAGE | Bacillus subtilis | 1 | ALA4477175 | assay format | Scientific Literature | |
5. | ALA4477470 | B | Activation of Bacillus subtilis ClpP expressed in Escherichia coli BL21 (DE3) using beta casein as substrate at 25 uM preincubated for 15 mins followed by substrate addition measured every 30 mins by SDS-PAGE | Bacillus subtilis | 1 | ALA4477175 | assay format | Scientific Literature |