| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA2148637 | B | Inhibition of rat ROMK channel expressed in HEk293 cells assessed as inhibition of thallium efflux by fluorescence assay | Rattus norvegicus | 1 | cell-based format | Scientific Literature | ||
| 2. | ALA2447658 | B | Inhibition of rat ROMK channel-mediated 86Rb+ flux | Rattus norvegicus | 2 | single protein format | Scientific Literature | ||
| 3. | ALA3615712 | B | Inhibition of rat ROMK by thallium flux assay | Rattus norvegicus | 1 | single protein format | Scientific Literature | ||
| 4. | ALA3705940 | B | Electrophysiology Assay: Block of Kir1.1 (ROMKI) currents was examined by whole cell voltage clamp (Hamill et. al. Pfluegers Archives 391:85-100 (1981)) using the IonWorks Quattro automated electrophysiology platform (Molecular Devices, Sunnyvale, Calif.). Chinese hamster ovary cells stably expressing Kir1.1 channels were maintained in T-75 flasks in cell culture media in a humidified 10% CO2 incubator at 37° C. Prior to an experiment, Kir1.1 expression was induced by overnight incubation with 1 mM sodium butyrate. On the day of the experiment, cells were dissociated with 2.5 ml of Versene (Invitrogen 15040-066) for approximately 6 min at 37° C. and suspended in 10 ml of bath solution containing (in mM): 150 NaCl, 10 KCl, 2.7 CaCl2, 0.5 MgCl2, 5 HEPES, pH 7.4. After centrifugation, the cell pellet was resuspended in approximately 4.0 ml of bath solution and placed in the IonWorks instrument. The intracellular solution consisted of (in mM): 80 K gluconate, 40 KCl, 20 KF, 3.2 MgCl2, 3 EGTA, 5 Hepes, pH 7.4. | 11 | cell-based format | BindingDB Database | |||
| 5. | ALA3705821 | B | Electrophysiology Assay: Block of Kir1.1 (ROMK1) currents was examined by whole cell voltage clamp (Hamill et. al. Pfluegers Archives 391:85-100 (1981)) using the IonWorks Quattro automated electrophysiology platform (Molecular Devices, Sunnyvale, Calif.). Chinese hamster ovary cells stably expressing Kir1.1 channels were maintained in T-75 flasks in cell culture media in a humidified 10% CO2 incubator at 37 C. Prior to an experiment, Kir1.1 expression was induced by overnight incubation with 1 mM sodium butyrate. On the day of the experiment, cells were dissociated with 2.5 mL of Versene (Invitrogen 15040-066) for approximately 6 min at 37 C. and suspended in 10 mL of bath solution containing (in mM): 150 NaCl, 10 KCl, 2.7 CaCl2, 0.5 MgCl2, 5 HEPES, pH 7.4. After centrifugation, the cell pellet was resuspended in approximately 4.0 mL of bath solution and placed in the IonWorks instrument. The intracellular solution consisted of (in mM): 80 K gluconate, 40 KCl, 20 KF, 3.2 MgCl2, 3 EGTA, 5 Hepes, pH 7.4. | 87 | cell-based format | BindingDB Database | |||
| 6. | ALA3705822 | B | Thallium Flux Assay: The ROMK channel functional thallium flux assay is performed in 384 wells, using the FLIPR-Tetra instrument. HEK-hKir1.1 cells are seeded in Poly-D-Lysine microplates and kept in a 37 C.-10% CO2 incubator overnight. On the day of the experiment, the growth media is replaced with the FluxOR reagent loading buffer and incubated, protected from light, at ambient temperature (23-25 C.) for 90 min. The loading buffer is replaced with assay buffer+/-test compound followed by 30 min incubation at ambient temperature, where the Thallium/Potassium stimulant is added to the microplate.Step Protocol 1. Seed HEK-hKir1.1 cells (50 ul at 20,000 cells/well) in 384-well PDL coated Microplates 2. Allow cells to adhere overnight in humidified 37 C./10% CO2 incubator 3. Completely remove cell growth media from microplate and replace with 25 ul loading buffer 4. Incubate Microplate at room temperature, protected form light, for 90 min 5. | 89 | assay format | BindingDB Database | |||
| 7. | ALA3705774 | B | Electrophysiology Assay: Block of Kir 1.1 (ROMKl) currents was examined by whole cell voltage clamp (Hamill et. al. Pfluegers Archives 391 : 85- 100 (1981)) using the IonWorks Quattro automated electrophysiology platform (Molecular Devices, Sunnyvale, CA). Chinese hamster ovary cells stably expressing Kirl.l channels were maintained in T-75 flasks in cell culture media in a humidified 10% CO2 incubator at 37 0C. Prior to an experiment, Kirl .l expression was induced by overnight incubation with 1 mM sodium butyrate. On the day of the experiment, cells were dissociated with 2.5 ml of Versene (Invitrogen 15040-066) for approximately 6 min at 37 0C and suspended in 10 ml of bath solution containing (in roM): 150 NaCl, 10 KCl, 2.7 CaCl2, 0.5 MgCl2, 5 HEPES5 pH 7.4. After centrifugation, the cell pellet was resuspended in approximately 4.0 ml of bath solution and placed in the Ion Works instrument. The intracellular solution consisted of (in mM): 80 K gluconate, 40 KCl, 20 KF5 3.2 MgCl2, 3 EGTA, 5 Hepes, pH 7.4. | 9 | cell-based format | BindingDB Database | |||
| 8. | ALA3797008 | B | Inhibition of rat ROMK expressed in HEK293 cells after 30 mins by [86Rb+] flux functional assay | Rattus norvegicus | 27 | cell-based format | Scientific Literature | ||
| 9. | ALA3795960 | B | Inhibition of rat ROMK assessed as thallium flux after 30 mins in presence of ouabain by cell based FLIPR assay | Rattus norvegicus | 1 | cell-based format | Scientific Literature | ||
| 10. | ALA4004328 | B | Inhibition of rat ROMK expressed in HEK293 cells after 30 mins by thallium flux assay | Rattus norvegicus | 5 | cell-based format | Scientific Literature | ||
| 11. | ALA4030048 | B | Inhibition of rat ROMK expressed in HEK293 cells after 30 mins by FLIPR based thallium flux assay | Rattus norvegicus | 1 | cell-based format | Scientific Literature | ||
| 12. | ALA4145000 | B | Inhibition of rat ROMK2 expressed in HEK293 cells at -75 mV holding potential by whole cell patch clamp method | Rattus norvegicus | 2 | cell-based format | Scientific Literature | ||
| 13. | ALA4145003 | B | Inhibition of rat ROMK1 expressed in HEK293 cells after 20 mins by Thallos-AM dye based fluorescence assay | Rattus norvegicus | 4 | cell-based format | Scientific Literature | ||
| 14. | ALA4322062 | B | Inhibition of rat ROMK1 expressed in HEK293 cells by Tl+ flux assay | Rattus norvegicus | 8 | cell-based format | Scientific Literature | ||
| 15. | ALA5039911 | B | Inhibition of rat Kir1.1 expressed in HEK293 cells by electrophysiology assay | Rattus norvegicus | 1 | cell-based format | Scientific Literature | ||
| 16. | ALA5233434 | B | Inhibition of rat ROMK | Rattus norvegicus | 1 | single protein format | Scientific Literature |
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