# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA2156467 | B | Inhibition of Francisella tularensis subsp. tularensis SCHU4 N-terminal His-tagged FabI-NADH complex expressed in Escherichia coli BL21(DE3) using crotonyl-CoA as substrate in presence of 200 uM NAD+ | Francisella tularensis subsp. tularensis SCHU S4 | 1 | ALA2151032 | assay format | Scientific Literature | |
2. | ALA2156468 | B | Competitive inhibition of Francisella tularensis subsp. tularensis SCHU4 N-terminal His-tagged FabI expressed in Escherichia coli BL21(DE3) using crotonyl-CoA as substrate by Dixon plot analysis in presence of 200 uM NADH | Francisella tularensis subsp. tularensis SCHU S4 | 1 | ALA2151032 | assay format | Scientific Literature | |
3. | ALA2156469 | B | Uncompetitive inhibition of Francisella tularensis subsp. tularensis SCHU4 N-terminal His-tagged FabI expressed in Escherichia coli BL21(DE3) using NADH as substrate by Dixon plot analysis in presence of 500 uM crotonyl-CoA | Francisella tularensis subsp. tularensis SCHU S4 | 1 | ALA2151032 | assay format | Scientific Literature | |
4. | ALA2400303 | B | Inhibition of Francisella tularensis SCHU S4 FabI F203L mutant using CrCoA as substrate by UV-vis spectrophotometric analysis in presence of non pre-equilibrated NAD+ | Francisella tularensis subsp. tularensis SCHU S4 | 4 | ALA2396561 | assay format | Scientific Literature | |
5. | ALA2401002 | B | Inhibition of Francisella tularensis SCHU S4 FabI R96M mutant using CrCoA as substrate by UV-vis spectrophotometric analysis in presence of non pre-equilibrated NAD+ | Francisella tularensis subsp. tularensis SCHU S4 | 4 | ALA2396561 | assay format | Scientific Literature | |
6. | ALA2401003 | B | Inhibition of Francisella tularensis SCHU S4 FabI R96G mutant using CrCoA as substrate by UV-vis spectrophotometric analysis in presence of non pre-equilibrated NAD+ | Francisella tularensis subsp. tularensis SCHU S4 | 4 | ALA2396561 | assay format | Scientific Literature | |
7. | ALA2401004 | B | Inhibition of Francisella tularensis SCHU S4 FabI F93V mutant using CrCoA as substrate by UV-vis spectrophotometric analysis in presence of non pre-equilibrated NAD+ | Francisella tularensis subsp. tularensis SCHU S4 | 4 | ALA2396561 | assay format | Scientific Literature | |
8. | ALA2401005 | B | Inhibition of C-terminal His6-tagged wild type Francisella tularensis SCHU S4 FabI expressed in Escherichia coli BL21 (DE3) using CrCoA as substrate by UV-vis spectrophotometric analysis in presence of non pre-equilibrated NAD+ | Francisella tularensis subsp. tularensis SCHU S4 | 4 | ALA2396561 | assay format | Scientific Literature | |
9. | ALA2401006 | B | Inhibition of C-terminal His6-tagged Francisella tularensis SCHU S4 FabI expressed in Escherichia coli BL21 (DE3) using CrCoA as substrate assessed as oxidation of NADH to NAD+ by fluorescence assay | Francisella tularensis subsp. tularensis SCHU S4 | 8 | ALA2396561 | assay format | Scientific Literature | |
10. | ALA2401007 | B | Competitive inhibition of C-terminal His6-tagged Francisella tularensis SCHU S4 FabI expressed in Escherichia coli BL21 (DE3) using CrCoA as substrate in presence of NAD+ | Francisella tularensis subsp. tularensis SCHU S4 | 2 | ALA2396561 | assay format | Scientific Literature |