# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA2162864 | B | Inhibition of recombinant USP7 expressed in Sf9 cells by Ub-CHOP reporter assay | 13 | ALA2157895 | cell-based format | Scientific Literature | ||
2. | ALA2163017 | B | Inhibition of USP7 in human HCT116 cells assessed as increase in p53 level at 30 uM incubated up to 48 hrs by Western blot analysis relative to control | Homo sapiens | 1 | ALA2157895 | cell-based format | Scientific Literature | |
3. | ALA2163018 | B | Inhibition of USP7 in human HCT116 cells assessed as increase in p21 level at 30 uM incubated up to 48 hrs by Western blot analysis relative to control | Homo sapiens | 1 | ALA2157895 | cell-based format | Scientific Literature | |
4. | ALA2163020 | B | Ratio of P005094 EC50 to compound EC50 for recombinant USP7 | 1 | ALA2157895 | single protein format | Scientific Literature | ||
5. | ALA2400587 | B | Inhibition of USP7 (unknown origin) using ubiquitin-EKL as substrate at 20 uM | Homo sapiens | 2 | ALA2396580 | single protein format | Scientific Literature | |
6. | ALA2400588 | B | Inhibition of USP7 (unknown origin) using ubiquitin-EKL as substrate | Homo sapiens | 4 | ALA2396580 | single protein format | Scientific Literature | |
7. | ALA3239925 | B | Inhibition of human USP7 using Ub-rhodamine 110 as substrate at 31 uM | Homo sapiens | 4 | ALA3232931 | single protein format | Scientific Literature | |
8. | ALA3239934 | B | Inhibition of human USP7 using Ub-rhodamine 110 as substrate | Homo sapiens | 4 | ALA3232931 | single protein format | Scientific Literature | |
9. | ALA3412280 | B | Inhibition of USP7 (unknown origin) by Ub-AMC assay | Homo sapiens | 1 | ALA3407357 | single protein format | Scientific Literature | |
10. | ALA3412281 | B | Inhibition of human USP7 by Ub-AMC assay | Homo sapiens | 2 | ALA3407357 | single protein format | Scientific Literature | |
11. | ALA3412282 | B | Inhibition of USP7 (unknown origin) by Ub-CHOP reporter assay | Homo sapiens | 2 | ALA3407357 | single protein format | Scientific Literature | |
12. | ALA3412283 | B | Inhibition of USP7 in multiple myeloma cells (unknown origin) by immunohistochemistry | Homo sapiens | 2 | ALA3407357 | cell-based format | Scientific Literature | |
13. | ALA4001936 | B | Inhibition of USP7 (unknown origin) using Ub-Rho as substrate preincubated for 30 mins followed by substrate addition and subsequent incubation in dark for 3 hrs by fluorescence assay | Homo sapiens | 22 | ALA4000237 | single protein format | Scientific Literature | |
14. | ALA4033114 | B | Binding affinity to human His-tagged and [15N-13C-2H], delta1[13CH3]-Ile, [13CH3]-Leu/Val and [13CH3]-Met-labeled USP7-catalytic domain (208 to 554 residues) expressed in Escherichia coli Rosetta 2 (DE3) cells by 2D[1H-15N]-based TROSY spectrum NMR analysis | Homo sapiens | 6 | ALA4028910 | cell-based format | Scientific Literature | |
15. | ALA4033115 | B | Inhibition of native full length C-terminal USP7 (unknown origin) using ubiquitin-Rho110 as substrate pre-incubated for 1 hr followed by substrate addition by fluorescence assay | Homo sapiens | 27 | ALA4028910 | single protein format | Scientific Literature | |
16. | ALA4033116 | B | Inhibition of USP7 in human SJSA cells assessed as increase in ubiquitinated MDM2 level after 16 hrs in presence of MG132 by MSD assay | Homo sapiens | 5 | ALA4028910 | cell-based format | Scientific Literature | |
17. | ALA4033117 | B | Inhibition of USP7 in human EOL-1 cells assessed as increase in p53 levels at 1.3 to 5 uM after 4 hrs by Western blot analysis relative to control | Homo sapiens | 3 | ALA4028910 | cell-based format | Scientific Literature | |
18. | ALA4033118 | B | Inhibition of USP7 in human EOL-1 cells assessed as upregulation of p21 levels at 1.3 to 5 uM after 4 hrs by Western blot analysis relative to control | Homo sapiens | 4 | ALA4028910 | cell-based format | Scientific Literature | |
19. | ALA4119759 | B | Inhibition of full length wild-type human N-terminal His-tagged USP7 expressed in baculovirus infected Sf9 cells using Ub-AMC as substrate preincubated for 30 mins followed by substrate addition measured after 2 hrs by fluorescence based assay | Homo sapiens | 1 | ALA4118084 | cell-based format | Scientific Literature | |
20. | ALA4119762 | B | Inhibition of human full-length wild-type N-terminal His-tagged USP7 expressed in baculovirus infected Sf9 cells using Ub-AMC as substrate preincubated for 30 mins followed by substrate addition measured after 60 mins by fluorescence based assay | Homo sapiens | 1 | ALA4118084 | cell-based format | Scientific Literature |