#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA2163014	B	Inhibition of HIs6-tagged USP21 by Ub-CHOP reporter assay		1	ALA2157895	single protein format	Scientific Literature
2.	ALA2400584	B	Inhibition of core catalytic domain of USP21 (unknown origin) using ubiquitin-Rh110 as substrate	Homo sapiens	1	ALA2396580	single protein format	Scientific Literature
3.	ALA3239928	B	Inhibition of human USP21 using Ub-rhodamine 110 as substrate at 31 uM	Homo sapiens	4	ALA3232931	single protein format	Scientific Literature
4.	ALA3239937	B	Inhibition of human USP21 using Ub-rhodamine 110 as substrate	Homo sapiens	4	ALA3232931	single protein format	Scientific Literature
5.	ALA4605983	B	Inhibition of human GST-tagged USP21 CD (196 to 565 residues) expressed in Escherichia coli assessed as cleavage of Ubiquitin-Rhodamine110-glycine to Ubiquitin and Rhodamine110-glycine using Ubiquitin-Rhodamine110-glycine as substrate by fluorescence based analysis	Homo sapiens	1	ALA4602719	assay format	Scientific Literature
6.	ALA4674638	B	Inhibition of USP21 (unknown origin) at 10 uM relative to control	Homo sapiens	2	ALA4673213	single protein format	Scientific Literature
7.	ALA5251097	B	Inhibition of human full length USP21 using Btn-Ahx-PNIRFLD-K(Ubi)-LPQQT-GD-amide as substrate preincubated for 15 to 20 mins followed by substrate addition and measured after 25 mins by HTRF assay	Homo sapiens	22	ALA5247643	single protein format	Scientific Literature
8.	ALA5251098	B	Inhibition of human USP21 using ubiquitin rhodamine 110 110 as substrate preincubated for 15 to 20 mins followed by enzyme addition and measured after 25 mins by fluorometric assay	Homo sapiens	22	ALA5247643	single protein format	Scientific Literature
9.	ALA5251099	B	Inhibition of human USP21 using ubiquitin aminoluciferin as substrate incubated for 25 mins by luminescence based assay	Homo sapiens	1	ALA5247643	single protein format	Scientific Literature
10.	ALA5251100	B	Binding affinity to recombinant human USP21 assessed as change in melting temperature at 100 uM by thermal shift assay	Homo sapiens	4	ALA5247643	single protein format	Scientific Literature
11.	ALA5251101	B	Binding affinity to recombinant human N-terminal His6-tagged USP21 (209 to 563 residues) expressed in Escherichia coli BL21 (DE3) assessed as dissociation constant by SPR analysis	Homo sapiens	3	ALA5247643	assay format	Scientific Literature
12.	ALA5251104	B	Inhibition of human USP21 (209 to 563 residues) expressed in Escherichia coli BL21 (DE3) using ubiquitin rhodamine 110 as substrate assessed as remaining enzyme activity at 10 to 50 uM by fluorometric assay	Homo sapiens	1	ALA5247643	assay format	Scientific Literature
13.	ALA5251117	B	Inhibition of human full length USP21 using Btn-Ahx-PNIRFLD-K(Ubi)-LPQQT-GD-amide as substrate assessed as maximal response preincubated for 15 to 20 mins followed by substrate addition and measured after 25 mins by HTRF assay	Homo sapiens	11	ALA5247643	single protein format	Scientific Literature
14.	ALA5251118	B	Inhibition of human USP21 using ubiquitin rhodamine 110 as substrate assessed as maximal response preincubated for 15 to 20 mins followed by enzyme addition and measured after 25 mins by fluorometric assay	Homo sapiens	8	ALA5247643	single protein format	Scientific Literature
15.	ALA5251119	B	Inhibition of full-length human USP21 transfected in human HEK293T cells co-transfected with NF-kappaB reporter firefly luciferase plasmid assessed as NF-kappaB activation at 10 uM and measured after 20 hrs by dual-luciferase reporter gene assay	Homo sapiens	4	ALA5247643	cell-based format	Scientific Literature
16.	ALA5251120	B	Binding affinity to C-terminally NanoLuc HiBiT-tagged USP21 transfected in human HEK293T cells assessed as increase in thermal stabilization at 49 degreeC at 50 to 100 uM incubated for 1 hrs by Nano-Glo substrate based CESTA analysis relative to control	Homo sapiens	5	ALA5247643	cell-based format	Scientific Literature
17.	ALA5251124	B	Displacement of wild-type Ubiquitin from recombinant human N-terminal His6-tagged USP21 (209 to 563 residues) expressed in Escherichia coli BL21 (DE3) by SPR competition binding assay	Homo sapiens	2	ALA5247643	assay format	Scientific Literature
18.	ALA5251125	B	Binding affinity to C-terminally NanoLuc HiBiT-tagged USP21 transfected in human HEK293T cells assessed as melting temperature at 10 uM incubated for 1 hrs by CESTA analysis	Homo sapiens	2	ALA5247643	cell-based format	Scientific Literature
19.	ALA5251126	B	Binding affinity to C-terminally NanoLuc HiBiT-tagged USP21 transfected in human HEK293T cells ssessed as thermal stability at 49 degreeC incubated for 1 hrs by CESTA analysis	Homo sapiens	2	ALA5247643	cell-based format	Scientific Literature
20.	ALA5251127	B	Inhibition of full-length human USP21 transfected in human HEK293T cells co-transfected with NF-kappaB reporter firefly luciferase plasmid assessed as NF-kappaB activation and measured after 20 hrs by dual-luciferase reporter gene assay	Homo sapiens	2	ALA5247643	cell-based format	Scientific Literature