# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA2163015 | B | Inhibition of USP28 by Ub-CHOP reporter assay | 1 | single protein format | Scientific Literature | |||
2. | ALA4356420 | B | Inhibition of USP28 (unknown origin) | Homo sapiens | 1 | single protein format | Scientific Literature | ||
3. | ALA4358894 | B | Inhibition of USP28 in human HeLa cell extracts assessed as inhibition of HA-Ub-C2Br probe labeling at 1 to 100 uM incubated for 30 mins by immunoblot analysis | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
4. | ALA4358899 | B | Covalent binding affinity to USP28 in human U2OS cells at 20 uM using probe-1 by ABPP analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
5. | ALA4372596 | B | Inhibition of USP28 in HEK293T cell lysates at 25 uM using HA-tagged vinyl-sulfone as substrate preincubated for 19 to 60 mins followed by substrate addition and measured after 19 to 25 mins by Western blot analysis | Homo sapiens | 1 | subcellular format | Scientific Literature | ||
6. | ALA4372606 | B | Inhibition of USP28 in HEK293T cell lysates at 25 uM using HA-tagged vinyl-sulfone as substrate measured after 2 to 30 mins by Western blot analysis | Homo sapiens | 1 | subcellular format | Scientific Literature | ||
7. | ALA4418451 | B | Inhibition of TEV-cleavable hexa-histidine tagged USP28 (1 to 1077 residues) (unknown origin) using ubiquitin-rhodamine 110 as substrate preincubated for 30 mins followed by substrate addition and measured after 20 mins by fluorescence based assay | Homo sapiens | 21 | single protein format | Patent Bioactivity Data | ||
8. | ALA4477145 | B | Inhibition of TEV-cleavable hexa-histidine tagged USP28 (1 to 1077 residues) (unknown origin) using ubiquitin-rhodamine 110 as substrate and measured after 90 mins by fluorescence based assay | Homo sapiens | 223 | single protein format | Patent Bioactivity Data | ||
9. | ALA4605985 | B | Inhibition of human GST-tagged USP28 expressed in Escherichia coli assessed as cleavage of Ubiquitin-Rhodamine110-glycine to Ubiquitin and Rhodamine110-glycine using Ubiquitin-Rhodamine110-glycine as substrate by fluorescence based analysis | Homo sapiens | 1 | assay format | Scientific Literature | ||
10. | ALA4674635 | B | Inhibition of USP28 (unknown origin) at 10 uM relative to control | Homo sapiens | 2 | single protein format | Scientific Literature | ||
11. | ALA4687441 | B | Inhibition of USP28 (unknown origin) at 20 uM | Homo sapiens | 2 | single protein format | Scientific Literature | ||
12. | ALA5153784 | B | Inhibition of USP28 (unknown origin) at 10 uM using ubiquitin-rhodamine 110 as substrate preincubated for 30 mins followed by substrate addition and measured after 2 hrs by microplate spectrophotometer analysis | Homo sapiens | 6 | single protein format | Scientific Literature | ||
13. | ALA5234107 | B | Inhibition of USP28 (unknown origin) using Ub-Rho100 as substrate by calorimetric analysis | Homo sapiens | 4 | single protein format | Scientific Literature | ||
14. | ALA5251163 | B | Inhibition of USP28 (unknown origin) using ubiquitin rhodamine 110 as substrate at 1 to 10 uM by DUBprofiler fluorometric assay | Homo sapiens | 2 | single protein format | Scientific Literature |