# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4356420 | Binding | Inhibition of USP28 (unknown origin) | Homo sapiens | 1 | ALA4354818 | single protein format | Scientific Literature | |
2. | ALA4358894 | Binding | Inhibition of USP28 in human HeLa cell extracts assessed as inhibition of HA-Ub-C2Br probe labeling at 1 to 100 uM incubated for 30 mins by immunoblot analysis | Homo sapiens | 2 | ALA4354861 | cell-based format | Scientific Literature | |
3. | ALA4358899 | Binding | Covalent binding affinity to USP28 in human U2OS cells at 20 uM using probe-1 by ABPP analysis | Homo sapiens | 1 | ALA4354861 | cell-based format | Scientific Literature | |
4. | ALA2163015 | Binding | Inhibition of USP28 by Ub-CHOP reporter assay | 1 | ALA2157895 | single protein format | Scientific Literature | ||
5. | ALA4372596 | Binding | Inhibition of USP28 in HEK293T cell lysates at 25 uM using HA-tagged vinyl-sulfone as substrate preincubated for 19 to 60 mins followed by substrate addition and measured after 19 to 25 mins by Western blot analysis | Homo sapiens | 1 | ALA4371052 | subcellular format | Scientific Literature | |
6. | ALA4372606 | Binding | Inhibition of USP28 in HEK293T cell lysates at 25 uM using HA-tagged vinyl-sulfone as substrate measured after 2 to 30 mins by Western blot analysis | Homo sapiens | 1 | ALA4371052 | subcellular format | Scientific Literature | |
7. | ALA4418451 | Binding | Inhibition of TEV-cleavable hexa-histidine tagged USP28 (1 to 1077 residues) (unknown origin) using ubiquitin-rhodamine 110 as substrate preincubated for 30 mins followed by substrate addition and measured after 20 mins by fluorescence based assay | Homo sapiens | 21 | ALA4418307 | single protein format | Patent Bioactivity Data | |
8. | ALA4477145 | Binding | Inhibition of TEV-cleavable hexa-histidine tagged USP28 (1 to 1077 residues) (unknown origin) using ubiquitin-rhodamine 110 as substrate and measured after 90 mins by fluorescence based assay | Homo sapiens | 223 | ALA4476884 | single protein format | Patent Bioactivity Data | |
9. | ALA4605985 | Binding | Inhibition of human GST-tagged USP28 expressed in Escherichia coli assessed as cleavage of Ubiquitin-Rhodamine110-glycine to Ubiquitin and Rhodamine110-glycine using Ubiquitin-Rhodamine110-glycine as substrate by fluorescence based analysis | Homo sapiens | 1 | ALA4602719 | assay format | Scientific Literature | |
10. | ALA4674635 | Binding | Inhibition of USP28 (unknown origin) at 10 uM relative to control | Homo sapiens | 2 | ALA4673213 | single protein format | Scientific Literature | |
11. | ALA4687441 | Binding | Inhibition of USP28 (unknown origin) at 20 uM | Homo sapiens | 2 | ALA4680269 | single protein format | Scientific Literature | |
12. | ALA5153784 | Binding | Inhibition of USP28 (unknown origin) at 10 uM using ubiquitin-rhodamine 110 as substrate preincubated for 30 mins followed by substrate addition and measured after 2 hrs by microplate spectrophotometer analysis | Homo sapiens | 6 | ALA5150063 | single protein format | Scientific Literature | |
13. | ALA5234107 | Binding | Inhibition of USP28 (unknown origin) using Ub-Rho100 as substrate by calorimetric analysis | Homo sapiens | 4 | ALA5233229 | single protein format | Scientific Literature | |
14. | ALA5251163 | Binding | Inhibition of USP28 (unknown origin) using ubiquitin rhodamine 110 as substrate at 1 to 10 uM by DUBprofiler fluorometric assay | Homo sapiens | 2 | ALA5247643 | single protein format | Scientific Literature |