| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA2163021 | B | Inhibition of USP8 by Ub-CHOP reporter assay | 1 | single protein format | Scientific Literature | |||
| 2. | ALA2400585 | B | Inhibition of core catalytic domain of USP8 (unknown origin) using ubiquitin-Rh110 as substrate | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 3. | ALA2411918 | B | Inhibition of human USP8 using Ub-AMC as substrate at 1 to 30 uM after 60 mins by fluorometric analysis | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 4. | ALA3239926 | B | Inhibition of human USP8 using Ub-rhodamine 110 as substrate at 31 uM | Homo sapiens | 4 | single protein format | Scientific Literature | ||
| 5. | ALA3239935 | B | Inhibition of human USP8 using Ub-rhodamine 110 as substrate | Homo sapiens | 4 | single protein format | Scientific Literature | ||
| 6. | ALA3269882 | B | Binding affinity to deubiquitinase USP08 (unknown origin) assessed as labeling after 4 hrs incubation | Homo sapiens | 4 | single protein format | Scientific Literature | ||
| 7. | ALA4119760 | B | Inhibition of human full-length His-tagged USP8 expressed baculovirus infected insect cells using Ub-AMC as substrate by fluorescence spectroscopic analysis | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 8. | ALA4119764 | B | Inhibition of USP8 (unknown origin) expressed in baculovirus infected Sf9 cells using Ub-AMC as substrate preincubated for 30 mins followed by substrate addition measured after 60 mins by fluorescence based assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 9. | ALA4310839 | B | Inhibition of recombinant USP8 catalytic domain (unknown origin) using Ubiquitin-Lys-TAMRA as substrate measured at 6 mins interval for 4 hrs by fluorescence assay | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 10. | ALA4310840 | B | Inhibition of recombinant USP8 catalytic domain (unknown origin) using Ubiquitin-Lys-TAMRA as substrate preincubated for 60 mins followed by substrate addition and measured at 6 mins interval for 4 hrs by fluorescence assay | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 11. | ALA4358854 | B | Inhibition of N-terminal His6-tagged full length recombinant human USP8 assessed as reduction in cleavage of luminescent substrate Ub-AML at 100 uM | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 12. | ALA4372595 | B | Inhibition of USP8 in HEK293T cell lysates at 25 uM using HA-tagged vinyl-sulfone as substrate preincubated for 19 to 60 mins followed by substrate addition and measured after 19 to 25 mins by Western blot analysis | Homo sapiens | 1 | subcellular format | Scientific Literature | ||
| 13. | ALA4372604 | B | Inhibition of USP8 in HEK293T cell lysates at 25 uM using HA-tagged vinyl-sulfone as substrate measured after 2 to 30 mins by Western blot analysis | Homo sapiens | 1 | subcellular format | Scientific Literature | ||
| 14. | ALA4372615 | B | Inhibition of USP8 in HEK293T cell lysates assessed as enzyme recovery at 250 uM using HA-tagged vinyl-sulfone as substrate preincubated for 40 mins followed by 10-fold dilution and subsequent substrate addition measured up to 2 hrs by Western blot analysis | Homo sapiens | 1 | subcellular format | Scientific Literature | ||
| 15. | ALA4605977 | B | Inhibition of human USP8 expressed in Escherichia coli assessed as cleavage of Ubiquitin-Rhodamine110-glycine to Ubiquitin and Rhodamine110-glycine using Ubiquitin-Rhodamine110-glycine as substrate by fluorescence based analysis | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 16. | ALA4674626 | B | Inhibition of USP8 (unknown origin) | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 17. | ALA4674645 | B | Inhibition of USP8 (unknown origin) at 10 uM relative to control | Homo sapiens | 2 | single protein format | Scientific Literature | ||
| 18. | ALA5112521 | B | Inhibition of N-terminal His-6 tagged USP8 (734 to 1110 residues) (unknown origin) expressed in Escherichia coli BL21 (DE3) cells at 10 nM measured after 3 hrs by fluorescence based Plate reader method relative to control | Homo sapiens | 68 | cell-based format | Scientific Literature | ||
| 19. | ALA5112522 | B | Inhibition of N-terminal His-6 tagged USP8 (734 to 1110 residues) (unknown origin) expressed in Escherichia coli BL21 (DE3) cells at 100 nM measured after 3 hrs by fluorescence based Plate reader method relative to control | Homo sapiens | 68 | cell-based format | Scientific Literature | ||
| 20. | ALA5112523 | B | Destabilization of N-terminal His-6 tagged USP8 (734 to 1110 residues) (unknown origin) expressed in Escherichia coli BL21 (DE3) cells assessed as change in melting temperature at 200 uM measured for 2 hrs in presence of dithiothreitol by SYPRO orange dye based thermal shift assay | Homo sapiens | 1 | cell-based format | Scientific Literature |
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