| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA2163021 | B | Inhibition of USP8 by Ub-CHOP reporter assay | 1 | single protein format | Scientific Literature | |||
| 2. | ALA5251109 | B | Inhibition of human USP8 (734 to 1110 residues) expressed in Escherichia coli BL21 (DE3) using ubiquitin rhodamine 110 as substrate assessed as remaining enzyme activity at 10 to 50 uM by fluorometric assay | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 3. | ALA5251140 | B | Inhibition of human USP8 using ubiquitin rhodamine 110 as substrate at 10 to 50 uM by fluorometric assay | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 4. | ALA5251151 | B | Inhibition of USP8 (unknown origin) using ubiquitin rhodamine 110 as substrate at 1 to 10 uM by DUBprofiler fluorometric assay | Homo sapiens | 2 | single protein format | Scientific Literature | ||
| 5. | ALA2400585 | B | Inhibition of core catalytic domain of USP8 (unknown origin) using ubiquitin-Rh110 as substrate | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 6. | ALA2411918 | B | Inhibition of human USP8 using Ub-AMC as substrate at 1 to 30 uM after 60 mins by fluorometric analysis | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 7. | ALA3239926 | B | Inhibition of human USP8 using Ub-rhodamine 110 as substrate at 31 uM | Homo sapiens | 4 | single protein format | Scientific Literature | ||
| 8. | ALA3239935 | B | Inhibition of human USP8 using Ub-rhodamine 110 as substrate | Homo sapiens | 4 | single protein format | Scientific Literature | ||
| 9. | ALA3269882 | B | Binding affinity to deubiquitinase USP08 (unknown origin) assessed as labeling after 4 hrs incubation | Homo sapiens | 4 | single protein format | Scientific Literature | ||
| 10. | ALA4119760 | B | Inhibition of human full-length His-tagged USP8 expressed baculovirus infected insect cells using Ub-AMC as substrate by fluorescence spectroscopic analysis | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 11. | ALA4119764 | B | Inhibition of USP8 (unknown origin) expressed in baculovirus infected Sf9 cells using Ub-AMC as substrate preincubated for 30 mins followed by substrate addition measured after 60 mins by fluorescence based assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 12. | ALA4310839 | B | Inhibition of recombinant USP8 catalytic domain (unknown origin) using Ubiquitin-Lys-TAMRA as substrate measured at 6 mins interval for 4 hrs by fluorescence assay | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 13. | ALA4310840 | B | Inhibition of recombinant USP8 catalytic domain (unknown origin) using Ubiquitin-Lys-TAMRA as substrate preincubated for 60 mins followed by substrate addition and measured at 6 mins interval for 4 hrs by fluorescence assay | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 14. | ALA4358854 | B | Inhibition of N-terminal His6-tagged full length recombinant human USP8 assessed as reduction in cleavage of luminescent substrate Ub-AML at 100 uM | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 15. | ALA4372595 | B | Inhibition of USP8 in HEK293T cell lysates at 25 uM using HA-tagged vinyl-sulfone as substrate preincubated for 19 to 60 mins followed by substrate addition and measured after 19 to 25 mins by Western blot analysis | Homo sapiens | 1 | subcellular format | Scientific Literature | ||
| 16. | ALA4372604 | B | Inhibition of USP8 in HEK293T cell lysates at 25 uM using HA-tagged vinyl-sulfone as substrate measured after 2 to 30 mins by Western blot analysis | Homo sapiens | 1 | subcellular format | Scientific Literature | ||
| 17. | ALA4372615 | B | Inhibition of USP8 in HEK293T cell lysates assessed as enzyme recovery at 250 uM using HA-tagged vinyl-sulfone as substrate preincubated for 40 mins followed by 10-fold dilution and subsequent substrate addition measured up to 2 hrs by Western blot analysis | Homo sapiens | 1 | subcellular format | Scientific Literature | ||
| 18. | ALA4605977 | B | Inhibition of human USP8 expressed in Escherichia coli assessed as cleavage of Ubiquitin-Rhodamine110-glycine to Ubiquitin and Rhodamine110-glycine using Ubiquitin-Rhodamine110-glycine as substrate by fluorescence based analysis | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 19. | ALA4674626 | B | Inhibition of USP8 (unknown origin) | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 20. | ALA4674645 | B | Inhibition of USP8 (unknown origin) at 10 uM relative to control | Homo sapiens | 2 | single protein format | Scientific Literature |
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