| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA2167518 | B | Inhibition of human KDM5C catalytic domain expressed in Sf9 cells using methyl lysine peptide substrate by AlphaScreen assay | Homo sapiens | 10 | ALA2163321 | cell-based format | Scientific Literature | |
| 2. | ALA3112252 | B | Inhibition of recombinant JARID1C (unknown origin) incubated for 15 mins prior to substrate addition by AlphaScreen method | Homo sapiens | 9 | ALA3108715 | single protein format | Scientific Literature | |
| 3. | ALA3624703 | B | Inhibition of KDM5C (unknown origin) using H3(1-21)K4Me3-GGK-biotin substrate incubated for 20 mins by alpha screen assay | Homo sapiens | 32 | ALA3621091 | single protein format | Scientific Literature | |
| 4. | ALA3624713 | B | Inhibition of KDM5C (unknown origin) | Homo sapiens | 3 | ALA3621091 | single protein format | Scientific Literature | |
| 5. | ALA3772972 | B | Inhibition of KDM5C catalytic domain (1 -764 aa) (unknown origin) expressed in Sf9 cells using H3K4Me3 peptide as substrate by RFMS assay | Homo sapiens | 6 | ALA3769320 | cell-based format | Scientific Literature | |
| 6. | ALA3772973 | B | Inhibition of human KDM5C transfected in U2OS cells assessed as inhibition of H3K9Me3 demethylation for 24 hrs | Homo sapiens | 6 | ALA3769320 | cell-based format | Scientific Literature | |
| 7. | ALA3778979 | B | Inhibition of KDM5C (unknown origin) using biotin-H3K4me3 as substrate preincubated for 15 mins followed by substrate addition measured after 20 mins by alphascreen assay | Homo sapiens | 54 | ALA3774338 | single protein format | Scientific Literature | |
| 8. | ALA3776212 | B | Inhibition of KDM5C (unknown origin) using biotin-H3K4me3 as substrate at 100 uM preincubated for 15 mins followed by substrate addition measured after 20 mins by alphascreen assay relative to control | Homo sapiens | 7 | ALA3774338 | single protein format | Scientific Literature | |
| 9. | ALA3779256 | B | Inhibition of full length human HALO-tagged KDM5C1 expressed in human U2OS cells assessed as level of H3K9Me3 demethylation incubated overnight by Hoechst 33342/HaloTag/ Alexa Fluor 647 staining-based image analysis | Homo sapiens | 4 | ALA3774367 | cell-based format | Scientific Literature | |
| 10. | ALA3779257 | B | Inhibition of KDM5C catalytic domain (1 to 764 residues) (unknown origin) expressed in Baculovirus-infected insect Sf9 cells using H3K4Me3 peptide as substrate assessed as demethylation of substrate preincubated for 10 mins followed by substrate addition in presence of alpha-ketoglutarate by Rapidfire mass spectrometric analysis | Homo sapiens | 4 | ALA3774367 | cell-based format | Scientific Literature | |
| 11. | ALA3791194 | B | Inhibition of KDM5C (unknown origin) after 24 hrs by alphascreen assay | Homo sapiens | 1 | ALA3784917 | single protein format | Scientific Literature | |
| 12. | ALA3791435 | B | Inhibition of KDM5C (unknown origin) preincubated for 15 mins followed by histone substrate addition measured after 1 hr by alphascreen assay | Homo sapiens | 1 | ALA3784917 | single protein format | Scientific Literature | |
| 13. | ALA3789213 | B | Inhibition of recombinant human N-terminal FLAG-tagged KDM5C (2 to 1560 residues) expressed in baculovirus infected sf9 cells using biotin-H3K4me3 as substrate preincubated for 10 mins followed by substrate addition measured after 10 mins by AlphaLisa assay | Homo sapiens | 3 | ALA3784917 | cell-based format | Scientific Literature | |
| 14. | ALA3789448 | B | Inhibition of KDM5C in human U2OS cells assessed as reduction in demethylation of H3K4me3 after 20 hrs by Hoechst 33342 staining based immunofluorescence assay | Homo sapiens | 3 | ALA3784917 | cell-based format | Scientific Literature | |
| 15. | ALA4035860 | B | Inhibition of recombinant human N-terminal FLAG-tagged KDM5C expressed in baculovirus infected Sf9 insect cells using H3K4me3 peptide as substrate after 30 to 45 mins by TR-FRET assay | Homo sapiens | 1 | ALA4033700 | cell-based format | Scientific Literature | |
| 16. | ALA4316555 | B | Inhibition of Flag-tagged KDM5C (unknown origin) expressed in Escherichia coli BL21 (DE3) assessed as formaldehyde release using core histone as substrate preincubated with enzyme for 10 mins followed by incubation with substrate for 1 hr measured following 2 hrs incubation with ammonium acetate/acetoacetanilide by fluorescence based analysis | Homo sapiens | 1 | ALA4312042 | assay format | Scientific Literature | |
| 17. | ALA4353691 | B | Inhibition of KDM5C (unknown origin) assessed as decrease in demethylation of substrate using peptide (H3(1-21)K4-Me3-GGKBiotin) as substrate and 2OG as cosubstrate pre-incubated for 15 mins before substrate addition followed by centrifugation for 15 secs and measured after 20 mins by Alphascreen assay | Homo sapiens | 33 | ALA4350996 | single protein format | Scientific Literature | |
| 18. | ALA4424785 | B | Inhibition of full-length human N-terminal FLAG/His-tagged KDM5C (2 to 1560 residues) expressed in baculovirus infected Sf9 insect cells using H3(1-21)K4(Me3)-GGK(Biotin)/2-OG as substrate measured after 1 hr by Alphalisa assay | Homo sapiens | 4 | ALA4422684 | cell-based format | Scientific Literature | |
| 19. | ALA4427163 | B | Inhibition of KDM5C (unknown origin) | Homo sapiens | 1 | ALA4425149 | single protein format | Scientific Literature | |
| 20. | ALA4427932 | B | Inhibition of KDM5C (unknown origin) using ART(Kme3)QTARKSTGGKAPRKQLA-NovaTagPEG-biotin/2-OG as substrate/co-factor by TR-FRET assay | Homo sapiens | 1 | ALA4425189 | single protein format | Scientific Literature |
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