| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA2166661 | B | Activity of human SIRT5 assessed as decrotonylation of compound at 50 uM after 1 hr by trypsin developer-based fluorescence assay | Homo sapiens | 2 | ALA2163301 | single protein format | Scientific Literature | |
| 2. | ALA2166863 | B | Inhibition of SIRT5 | 6 | ALA2163301 | single protein format | Scientific Literature | ||
| 3. | ALA2166865 | B | Ratio of Kcat to Km for human SIRT5 assessed as desuccinylation of compound after 1 hr by Michaelis-Menten plot analysis | Homo sapiens | 2 | ALA2163301 | single protein format | Scientific Literature | |
| 4. | ALA2166867 | B | Activity of human SIRT5 assessed as desuccinylation of compound after 1 hr by Michaelis-Menten plot analysis | Homo sapiens | 2 | ALA2163301 | single protein format | Scientific Literature | |
| 5. | ALA2166869 | B | Activity of human SIRT5 assessed as deacylation of compound at 50 uM after 1 hr by trypsin developer-based fluorescence assay | Homo sapiens | 7 | ALA2163301 | single protein format | Scientific Literature | |
| 6. | ALA2209184 | B | Inhibition of human SIRT5 using H3K9 succinyl peptide substrate | Homo sapiens | 1 | ALA2203031 | single protein format | Scientific Literature | |
| 7. | ALA2209185 | B | Inhibition of SIRT5 using ZK(suc)A substrate after 1 hr by homogeneous fluorescence assay | 22 | ALA2203031 | single protein format | Scientific Literature | ||
| 8. | ALA2320143 | B | Inhibition of human Sirt5 desuccinylation activity using SKEYFS-succinylLys-QK as substrate at 100 uM after 60 mins by glutamate dehydrpgenase-coupled assay | Homo sapiens | 7 | ALA2311504 | single protein format | Scientific Literature | |
| 9. | ALA2320144 | B | Inhibition of human Sirt5 desuccinylation activity using SKEYFS-succinylLys-QK as substrate assessed as activity remaining at 100 UM measured for 20 mins by MS analysis | Homo sapiens | 1 | ALA2311504 | single protein format | Scientific Literature | |
| 10. | ALA2320145 | B | Inhibition of human Sirt5 desuccinylation activity using SKEYFS-succinylLys-QK as substrate measured for 15 mins by MS analysis | Homo sapiens | 1 | ALA2311504 | single protein format | Scientific Literature | |
| 11. | ALA2320146 | B | Inhibition of human Sirt5 deacetylation activity using FKRGVL-acetylLys-EYGVKV as substrate assessed as activity remaining at 100 UM measured for 20 mins by MS analysis | Homo sapiens | 1 | ALA2311504 | single protein format | Scientific Literature | |
| 12. | ALA2320147 | B | Inhibition of human Sirt5 deacetylation activity using FKRGVL-acetylLys-EYGVKV as substrate measured for 15 mins by MS analysis | Homo sapiens | 1 | ALA2311504 | single protein format | Scientific Literature | |
| 13. | ALA2320148 | B | Selectivity ratio of IC50 for human Sirt5 deacetylation activity to IC50 for human Sirt5 desuccinylation activity | Homo sapiens | 1 | ALA2311504 | single protein format | Scientific Literature | |
| 14. | ALA2320149 | B | Inhibition of human Sirt5 deacetylation activity using FKRGVL-acetylLys-EYGVKV as substrate at 100 uM after 60 mins by glutamate dehydrogenase-coupled assay | Homo sapiens | 7 | ALA2311504 | single protein format | Scientific Literature | |
| 15. | ALA2320150 | B | Inhibition of human Sirt5 deacetylation activity using FKRGVL-acetylLys-EYGVKV as substrate after 60 mins by glutamate dehydrogenase-coupled assay | Homo sapiens | 1 | ALA2311504 | single protein format | Scientific Literature | |
| 16. | ALA2320151 | B | Inhibition of human Sirt5 deacetylation activity using SKEYFS-acetylLys-QK as substrate measured for 15 mins by MS analysis | Homo sapiens | 1 | ALA2311504 | single protein format | Scientific Literature | |
| 17. | ALA3214924 | B | PubChem BioAssay. MLPCN SirT-5 Measured in Biochemical System Using Imaging - 7044-01_Inhibitor_Dose_CherryPick_Activity_Set6. (Class of assay: confirmatory) | 42 | ALA1201862 | biochemical format | PubChem BioAssays | ||
| 18. | ALA3214958 | B | PubChem BioAssay. MLPCN SirT-5 Measured in Biochemical System Using Imaging - 7044-01_Inhibitor_Dose_CherryPick_Activity_Set5. (Class of assay: confirmatory) | 189 | ALA1201862 | biochemical format | PubChem BioAssays | ||
| 19. | ALA3405660 | B | Inhibition of human recombinant SIRT5 isoform 1 (34 to 302 amino acids) expressed in Escherichia coli BL21 (DE3) codon plus RIL cells assessed as reduction in deacetylase activity using acetylated chicken histone substrate and [3H]-NAD+ | Homo sapiens | 1 | ALA3400009 | cell-based format | Scientific Literature | |
| 20. | ALA3405661 | B | Inhibition of human SIRT5 assessed as reduction in desuccinylase activity using KQTAR(SuK)STGGKA substrate | Homo sapiens | 5 | ALA3400009 | single protein format | Scientific Literature |
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