#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA2166866	B	Activity of recombinant human SIRT7 assessed as deacylation of compound at 50 uM after 1 hr by trypsin developer-based fluorescence assay	Homo sapiens	7	ALA2163301	single protein format	Scientific Literature
2.	ALA3888542	B	Inhibition Assay: Different concentrations of TA, TB, TH, or TM were pre-incubated with 1 μM of the sirtuin and 1 mM NAD in 20 mM Tris-HCl buffer (pH 8.0) with 1 mM dithiothreitol (DTT) at 37° C. for 30 minutes. Then 0.05 mM acyl peptides (H3K9Ac for Sirt1, Sirt2 and Sirt3; H3K9Su for Sirt5; H3K9Myr for Sirt6 and Sirt7) were added to initiate the reactions. The total reaction volume iwas 60 mL. Then reactions were incubated at 37° C. for a certain amount of time (2 minutes for Sirt1, 10 minutes for Sirt2 and Sirt5, 30 minutes for Sirt3, and 1 hour for Sirt6 and Sirt7). The reactions were stopped by adding 60 mL of an aqueous solution containing 200 mM HCl and 320 mM acetic acid. After centrifugation to remove precipitated proteins, the supernatant was analyzed by HPLC with a reverse phase C18 column (250×4.6 mm, 90 A, 10 mm, GraceVydac, Southborough, Mass.), with a linear gradient of 0% to 20% B for 10 minutes (1 mL/min).		4	ALA3886754	single protein format	BindingDB Database
3.	ALA4156267	B	Inhibition of recombinant human N-terminal His6-tagged SIRT7 (68 to 354 residues) expressed in Escherichia coli Transetta(DE3) using AcIQF as substrate preincubated for 30 mins followed by substrate and NAD+ addition measured after 2 hrs by fluorescence assay	Homo sapiens	1	ALA4152324	assay format	Scientific Literature
4.	ALA4236297	B	Inhibition of SIRT7 (unknown origin)	Homo sapiens	5	ALA4229543	single protein format	Scientific Literature
5.	ALA4373295	B	Inhibition of tRNA-activated deacetylation activity of recombinant human full length N-terminal His6-tagged SIRT7 expressed in Escherichia coli using H2N-GGKAPR-[Nepsilon-acetyl-lysine]-QLATKA-CONH2 as substrate measured after 30 mins by HPLC analysis	Homo sapiens	3	ALA4371077	assay format	Scientific Literature
6.	ALA4628184	B	Inhibition of tRNA-activated His6-tagged human SIRT7 deacetylation activity using acetyl-lysine as substrate in presence of beta-NAD+ after 30 mins by reversed-phase HPLC analysis	Homo sapiens	1	ALA4627291	single protein format	Scientific Literature
7.	ALA4683753	A	Inhibition of SIRT7 (unknown origin) at 10 uM relative to control	Homo sapiens	11	ALA4680157	single protein format	Scientific Literature
8.	ALA4685921	B	Substrate activity at human N-terminal His-tagged SIRT7 (68 to 354 residues) expressed in Escherichia coli at 0.5 uM measured after 2 hrs in presence of NAD+ and trypsin by fluorescence assay	Homo sapiens	16	ALA4680222	assay format	Scientific Literature
9.	ALA4685927	B	Substrate activity at human N-terminal His-tagged SIRT7 (68 to 354 residues) expressed in Escherichia coli at 5 uM measured after 2 hrs in presence of NAD+ and trypsin by fluorescence assay	Homo sapiens	16	ALA4680222	assay format	Scientific Literature
10.	ALA4685933	B	Substrate activity at human N-terminal His-tagged SIRT7 (68 to 354 residues) expressed in Escherichia coli at 50 uM measured after 2 hrs in presence of NAD+ and trypsin by fluorescence assay	Homo sapiens	16	ALA4680222	assay format	Scientific Literature
11.	ALA4714814	B	Binding affinity to SIRT7 (unknown origin) assessed as increase in fluorescence intensity at 2.5 uM measured at 5 mins interval for 1 hr in presence of NAD+ by fluorometric method relative to control	Homo sapiens	12	ALA4706730	single protein format	Scientific Literature
12.	ALA5037919	B	Inhibition of human recombinant SIRT7 at 10 uM incubated for 20 mins followed by mixing and measure after 30 mins by Luminescence assay relative to control	Homo sapiens	8	ALA5036349	single protein format	Scientific Literature
13.	ALA5227988	B	Inhibition of SIRT7 (unknown origin)	Homo sapiens	1	ALA5226318	single protein format	Scientific Literature
14.	ALA5228016	B	Inhibition of SIRT7 (unknown origin) at 10 uM relative to control	Homo sapiens	1	ALA5226318	single protein format	Scientific Literature