# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA2166870 | B | Activity of recombinant human SIRT4 assessed as deacylation of compound at 50 uM after 1 hr by trypsin developer-based fluorescence assay | Homo sapiens | 7 | ALA2163301 | single protein format | Scientific Literature | |
2. | ALA4234603 | B | Activity at recombinant SIRT4 (unknown origin) in presence of NAD by LC-MS analysis | Homo sapiens | 6 | ALA4229465 | single protein format | Scientific Literature | |
3. | ALA4234604 | B | Ratio of Kcat to Km at recombinant SIRT4 (unknown origin) | Homo sapiens | 6 | ALA4229465 | single protein format | Scientific Literature | |
4. | ALA4234605 | B | Activity at human N-terminal His6-tagged SIRT4 (25 to 314 residues) expressed in Escherichia coli codon plus (DE3) | Homo sapiens | 4 | ALA4229465 | assay format | Scientific Literature | |
5. | ALA4234606 | B | Ratio of Kcat to Km at human N-terminal His6-tagged SIRT4 (25 to 314 residues) expressed in Escherichia coli codon plus (DE3) | Homo sapiens | 4 | ALA4229465 | assay format | Scientific Literature | |
6. | ALA4714811 | B | Binding affinity to SIRT4 (unknown origin) assessed as increase in fluorescence intensity at 2.5 uM measured at 5 mins interval for 1 hr in presence of NAD+ by fluorometric method relative to control | Homo sapiens | 12 | ALA4706730 | single protein format | Scientific Literature | |
7. | ALA5112745 | B | Activation of SIRT4 (unknown origin) at 10 uM using HMG-CPS1 as substrate | Homo sapiens | 1 | ALA5108071 | single protein format | Scientific Literature |