| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA2175347 | B | Activity of human recombinant cytochrome P450 reductase assessed as rate of superoxide generation measuring SOD-inhibitable reduction of succinoylated cytochrome c per 2 mU enzyme at 100 umol/ml by spectrophotometry in presence of catalase and DTPA (Rvb = 0.2 +/- 0.1 micomol/L/min) | Homo sapiens | 5 | ALA2169815 | single protein format | Scientific Literature | |
| 2. | ALA3767758 | B | Activity of NADPH:cytochrome P-450R (unknown origin) assessed as O2 uptake at 5 to 15 uM by polarographic analysis in the presence of 150 ug/ml superoxide dismutase and 150 to 200 uM of NADPH | Homo sapiens | 13 | ALA3763095 | single protein format | Scientific Literature | |
| 3. | ALA3767761 | B | Activity of NADPH:cytochrome P-450R (unknown origin) assessed as NADPH oxidation by spectrophotometric analysis | Homo sapiens | 13 | ALA3763095 | single protein format | Scientific Literature | |
| 4. | ALA3767963 | B | Activity of NADPH:cytochrome P-450R (unknown origin) assessed as O2 uptake at 5 to 15 uM by polarographic analysis in the presence of 100 U/ml catalase and 150 to 200 uM of NADPH | Homo sapiens | 13 | ALA3763095 | single protein format | Scientific Literature | |
| 5. | ALA3767227 | B | Activity at human recombinant CPR assessed as superoxide generation at 100 umol/ml by spectrophotometric assay | Homo sapiens | 7 | ALA3763096 | single protein format | Scientific Literature | |
| 6. | ALA4009115 | A | Substrate activity at CPR in human L02 cells assessed as CPR-mediated one-electron reduction of compound by measuring ROS production at 10 uM after 1 to 3 hrs by DCFH-DA staining based flow cytometry | Homo sapiens | 1 | ALA4007423 | cell-based format | Scientific Literature | |
| 7. | ALA4009116 | A | Substrate activity at CPR in human L02 cells assessed as CPR-mediated one-electron reduction of compound by measuring ROS production after 1 to 3 hrs by DCFH-DA staining based flow cytometry | Homo sapiens | 1 | ALA4007423 | cell-based format | Scientific Literature | |
| 8. | ALA4009125 | A | Substrate activity at CPR in human L02 cells assessed as CPR-mediated one-electron reduction of compound by measuring cell growth inhibition at 20 to 100 uM treated for >8 hrs measured after 72 hrs by MTT assay | Homo sapiens | 1 | ALA4007423 | cell-based format | Scientific Literature | |
| 9. | ALA4009128 | A | Substrate activity at CPR in human L02 cells assessed as CPR-mediated one-electron reduction of compound by measuring cell growth inhibition treated for 24 hrs measured after 72 hrs by MTT assay | Homo sapiens | 11 | ALA4007423 | cell-based format | Scientific Literature | |
| 10. | ALA4009136 | A | Substrate activity at recombinant human CPR expressed in baculovirus infected insect cells assessed as compound reduction rate by measuring NADPH consumption per umol of enzyme preincubated for 3 mins followed by NADPH addition measured for 5 mins at 2 sec time interval by UV-Visible spectroscopic method | Homo sapiens | 23 | ALA4007423 | cell-based format | Scientific Literature | |
| 11. | ALA4021189 | B | Induction of recombinant human CPR-mediated superoxide generation assessed as one-electron reduction of compound by measuring SOD-inhibitable reduction of succinoylated cytochrome c per 2 mU enzyme at 100 umol/ml by spectrophotometric analysis (Rvb = 0.2 +/- 0.1 micromol/L/min) | Homo sapiens | 7 | ALA4020720 | single protein format | Scientific Literature | |
| 12. | ALA4042823 | A | Prodrug activation assessed as supersomal P450 oxido-reductase (unknown origin)-mediated CA4 level at 2.5 uM in presence of 0.5% O2 condition after 2 mins by HPLC method | Homo sapiens | 1 | ALA4041538 | single protein format | Scientific Literature | |
| 13. | ALA4131165 | A | Substrate activity at human CPR expressed in baculovirus expression system assessed as reduction rate by measuring umol of NADPH utilized per umol of enzyme at 10 uM preincubated for 3 mins followed by NADPH addition measured at 2 secs interval for 5 mins by UV-Vis spectroscopy | Homo sapiens | 8 | ALA4130353 | assay format | Scientific Literature | |
| 14. | ALA4392600 | A | Prodrug activation in pH 7.4 PBS buffer assessed as 3.3 ug/mL NADPH-cytochrome P450 reductase (unknown origin) mediated FdUrd formation at 0.62 mM in the presence of beta-NADPH measured after 2 hrs under hypoxic condition by reverse phase HPLC relative to control | Homo sapiens | 2 | ALA4390692 | single protein format | Scientific Literature | |
| 15. | ALA4392601 | A | Prodrug activation in pH 7.4 PBS buffer assessed as 3.3 ug/mL NADPH-cytochrome P450 reductase (unknown origin) mediated FdUrd formation at 0.62 mM in the presence of beta-NADPH measured after 2 hrs under aerobic condition by reverse phase HPLC | Homo sapiens | 1 | ALA4390692 | single protein format | Scientific Literature | |
| 16. | ALA4392602 | A | Prodrug conversion assessed as NADPH-cytochrome P450 reductase (unknown origin) mediated F-OH formation by measuring Vmax at 0.93 mM in the presence of beta-NADPH measured after 15 mins under hypoxic condition by Lineweaver-Burk plot analysis | Homo sapiens | 1 | ALA4390692 | single protein format | Scientific Literature | |
| 17. | ALA4392603 | A | Prodrug conversion in pH 7.4 PBS buffer assessed as NADPH-cytochrome P450 reductase (unknown origin) mediated FdUrd release by measuring Vmax at 0.62 mM in the presence of beta-NADPH measured after 2 hrs under hypoxic condition by Lineweaver-Burk plot analysis | Homo sapiens | 1 | ALA4390692 | single protein format | Scientific Literature | |
| 18. | ALA4392604 | A | Prodrug conversion in pH 7.4 PBS buffer assessed as NADPH-cytochrome P450 reductase (unknown origin) mediated FdUrd release by measuring Vmax at 0.62 mM in the presence of beta-NADPH measured after 2 hrs under aerobic condition by Lineweaver-Burk plot analysis | Homo sapiens | 1 | ALA4390692 | single protein format | Scientific Literature | |
| 19. | ALA4392605 | A | Prodrug conversion in pH 7.4 PBS buffer assessed as NADPH-cytochrome P450 reductase (unknown origin) mediated FdUrd release by measuring Km at 0.62 mM in the presence of beta-NADPH measured after 2 hrs under hypoxic condition by Lineweaver-Burk plot analysis | Homo sapiens | 1 | ALA4390692 | single protein format | Scientific Literature | |
| 20. | ALA4392606 | A | Prodrug conversion in pH 7.4 PBS buffer assessed as NADPH-cytochrome P450 reductase (unknown origin) mediated FdUrd release by measuring Km at 0.62 mM in the presence of beta-NADPH measured after 2 hrs under aerobic condition by Lineweaver-Burk plot analysis | Homo sapiens | 1 | ALA4390692 | single protein format | Scientific Literature |
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