| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA2187439 | B | Binding affinity at human recombinant ARTD8 expressed in Escherichia coli BL21 (DE3) R3 pRARE cells by isothermal titration calorimetry | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 2. | ALA2187440 | B | Binding affinity at human recombinant ARTD8 expressed in Escherichia coli BL21 (DE3) R3 pRARE cells assessed as thermal stability shift by differential scanning fluorimetry | Homo sapiens | 17 | cell-based format | Scientific Literature | ||
| 3. | ALA3382579 | B | Inhibition of human recombinant PARP14 at 10 uM incubated for 1 hr using using NAD+, biotinylated NAD+ and activated DNA by luminescence based assay | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 4. | ALA3382589 | B | Inhibition of human recombinant PARP14 at 100 uM incubated for 1 hr using using NAD+, biotinylated NAD+ and activated DNA by luminescence based assay | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 5. | ALA3420114 | B | Inhibition of hexahistidine-tagged human recombinant ARTD8 catalytic domain (1611 to 1801 amino acids) expressed in Escherichia coli BL21(DE3) assessed as inhibition of ADP-ribosyltransferase activity incubated for 15 mins using biotin-NAD+ by chemiluminescence detection based assay | Homo sapiens | 18 | assay format | Scientific Literature | ||
| 6. | ALA3420118 | B | Inhibition of hexahistidine-tagged human recombinant ARTD8 catalytic domain (1611 to 1801 amino acids) expressed in Escherichia coli BL21(DE3) assessed as inhibition of ADP-ribosyltransferase activity at 200 uM incubated for 15 mins using biotin-NAD+ by chemiluminescence detection based assay | Homo sapiens | 8 | assay format | Scientific Literature | ||
| 7. | ALA3757405 | B | Inhibition of human ARTD8 by fluorescence analysis | Homo sapiens | 2 | single protein format | Scientific Literature | ||
| 8. | ALA3995370 | B | Inhibition of recombinant human His6-tagged PARP14 ADP-ribosyltransferase domain expressed in Escherichia coli BL21(DE3) preincubated for 15 mins followed by biotinylated NAD+ addition by chemiluminescence assay | Homo sapiens | 9 | assay format | Scientific Literature | ||
| 9. | ALA4003516 | B | Inhibition of PARP14 (unknown origin) | Homo sapiens | 7 | single protein format | Scientific Literature | ||
| 10. | ALA4012889 | B | Binding affinity to PARP14 in human Jurkat cell extract after 45 mins by mass spectrometric analysis | Homo sapiens | 5 | single protein format | Scientific Literature | ||
| 11. | ALA4022061 | B | Inhibition of recombinant human His6-tagged PARP14 catalytic/WWE domain (1459 to 1801 residues) expressed in Escherichia coli BL21(DE3) preincubated for 15 mins followed by biotinylated NAD+ addition by chemiluminescence assay | Homo sapiens | 18 | assay format | Scientific Literature | ||
| 12. | ALA4022062 | B | Inhibition of recombinant human His6-tagged PARP14 catalytic/WWE domain (1459 to 1801 residues) expressed in Escherichia coli BL21(DE3) at 5 uM preincubated for 15 mins followed by biotinylated NAD+ addition by chemiluminescence assay relative to control | Homo sapiens | 2 | assay format | Scientific Literature | ||
| 13. | ALA4032831 | B | Inhibition of human PARP14 using NAD+ as substrate by fluorescence assay | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 14. | ALA4122799 | B | Inhibition of full length recombinant His6-tagged human PARP14 WWA/catalytic domain expressed in Escherichia coli BL21(DE3) preincubated for 15 mins followed by biotinylated NAD+ addition by chemiluminescence assay | Homo sapiens | 15 | assay format | Scientific Literature | ||
| 15. | ALA4122800 | B | Inhibition of full length recombinant His6-tagged human PARP14 WWA/catalytic domain expressed in Escherichia coli BL21(DE3) at 5 uM preincubated for 15 mins followed by biotinylated NAD+ addition by chemiluminescence assay relative to control | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 16. | ALA4122806 | B | Inhibition of full length recombinant His6-tagged PARP14 catalytic domain (unknown origin) expressed in Escherichia coli BL21(DE3) by fluorescence based assay | Homo sapiens | 3 | assay format | Scientific Literature | ||
| 17. | ALA4222714 | B | Binding affinity to PARP14 MD2 (A994 to N1191 residues) (unknown origin) expressed in bacterial expression system by ITC assay | Homo sapiens | 33 | assay format | Scientific Literature | ||
| 18. | ALA4222715 | B | Displacement of ARTK(Bio)QTARK(Aoa-RADP)S from His6-tagged PARP14 MD2 (A994 to N1191 residues) (unknown origin) expressed in bacterial expression system after 30 mins by AlphaScreen assay | Homo sapiens | 79 | assay format | Scientific Literature | ||
| 19. | ALA4272749 | B | Inhibition of N-terminal His-tagged thioredoxin-fused human ARTD8 (1535 to 1801 residues) expressed in Escherichia coli Rosetta2 (DE3) after 21 hrs in presence of NAD+ | Homo sapiens | 5 | assay format | Scientific Literature | ||
| 20. | ALA4305051 | B | Inhibition of human N-terminal His/GST-tagged PARP14 (1470 to 1801 residues) expressed in baculovirus infected Sf9 insect cells at 500 nM using histone as substrate measured after 1 hr by horseradish peroxidase-coupled chemiluminescence assay relative to control | Homo sapiens | 5 | cell-based format | Scientific Literature |
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