| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA2187103 | F | Agonist activity at human TLR5 expressed in HEK293 cells assessed as induction of NF-kappaB activity by measuring extracellular secreted alkaline phosphatase up to 250 uM by HEK-blue reporter gene assay | Homo sapiens | 22 | cell-based format | Scientific Literature | ||
| 2. | ALA2389991 | B | Activation of human TLR5 transfected in HEK293 cells at 10 uM after 20 to 24 hrs by SEAP assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 3. | ALA2422061 | B | Antagonist activity at human TLR5 expressed in HEK293 cells co-transfected with MD2 and sAP assessed as inhibition of NF-kappaB activity by reporter gene assay | Homo sapiens | 4 | cell-based format | Scientific Literature | ||
| 4. | ALA2424006 | B | Modulation of human TLR5 expressed in HEK293 cells co-transfected with MD2 and sAP assessed as induction of NF-kappaB activity by reporter gene assay | Homo sapiens | 29 | cell-based format | Scientific Literature | ||
| 5. | ALA3270134 | B | Agonist activity at human recombinant TLR5 expressed in HEK293 cells at 10 uM by SEAP reporter assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 6. | ALA3365536 | B | Agonist activity at human TLR5 stably expressed in HEK293 cells co-expressing secreted alkaline phosphatase assessed as NF-kappaB induction by liquid handler-assisted reporter gene assay | Homo sapiens | 33 | cell-based format | Scientific Literature | ||
| 7. | ALA4008414 | B | Activity at human TLR5 expressed in HEK293 cells co-expressing secreted alkaline phosphatase assessed as induction of NF-kappaB/AP-1 promoter activity by measuring increase in secreted alkaline phosphatase level by spectrophotometric method | Homo sapiens | 38 | cell-based format | Scientific Literature | ||
| 8. | ALA4016464 | B | Agonist activity at human TLR5 expressed in HEK293 cells assessed as induction of NF-kB activation-mediated SEAP production after 24 hrs by HTS assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 9. | ALA4195477 | B | Antagonist activity at human TLR5 expressed in HEK293 cells assessed as decrease in flagellin-induced NF-kappaB activation-mediated SEAP production at 10'-9 to 10'-5 M after 24 hrs by Quanti-blue-based assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 10. | ALA4306987 | B | Agonist activity at TLR5 in human THP1 cells assessed as inhibition of flagellin-induced IL-8 secretion preincubated for 1 hr followed by flagellin stimulation and measured after 24 hrs by ELISA | Homo sapiens | 3 | cell-based format | Scientific Literature | ||
| 11. | ALA4359301 | B | Inhibition of human TLR5 expressed in HEK-Blue cells assessed as inhibition of flagellin-induced NF-kappaB activation at 20 uM measured after 24 hrs by quanti-blue based SEAP reporter gene assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 12. | ALA4429199 | B | Agonist activity at human TLR5 expressed in HEK293 cells assessed as induction of NF-kappaB activity by secreted alkaline phosphatase reporter gene assay | Homo sapiens | 7 | cell-based format | Scientific Literature | ||
| 13. | ALA4429324 | B | Agonist activity at wild-type human TLR5 expressed in HEK293 cells up to 25 uM measured after 6 hrs by NF-kappaB luciferase reporter gene assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 14. | ALA4622264 | B | Antagonist activity at TLR5 in human PBMC assessed as inhibition of flagellin-induced TNFalpha production preincubated for 30 mins followed by flagellin addition and measured after 20 hrs by HTRF assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 15. | ALA4687757 | B | Inhibition of Flagellin-BS binding to human TLR5 expressed in HEK293 cells harboring SEAP reporter gene preincubated for 24 hrs followed by compound wash out and subsequent Flagellin-BS addition along with compound and measured after 0.5 hrs by QUANTI-Blue assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 16. | ALA4726030 | B | Agonist activity at human TLR5 expressed in HEK293 cells assessed as increase in NF-kappaB activation up to 10 uM incubated for 24 hrs by quanti-blue SEAP reporter gene assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 17. | ALA4765521 | B | Agonist activity at human TLR5 stably expressed in HEK293 cells cotransfected with secreted alkaline phosphatase assessed as induction of NFkappaB activation by quanti-blue SEAP reporter gene based spectrophotometric method | Homo sapiens | 43 | cell-based format | Scientific Literature | ||
| 18. | ALA4765528 | B | Inhibition of human TLR5 stably expressed in HEK293 cells cotransfected with secreted alkaline phosphatase assessed as assessed as inhibition of flagellin-induced NF-kappaB activation by quanti-blue SEAP reporter gene based spectrophotometric method | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 19. | ALA5039716 | B | Agonist activity at TLR5 in HEK-Blue hTLR5 cells at 0.1 to 100 uM incubated for 24 hrs by Quanti-Blue based SEAP assay | Homo sapiens | 1 | cell-based format | Scientific Literature |
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