#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA1914129	Binding	Inhibition of mouse recombinant PI3K p110alpha expressed in Sf21 insect cells using phosphatidylinositol as substrate after 1 hr by phosphoimaging	Mus musculus	42	ALA1909493	cell-based format	Scientific Literature
2.	ALA1942055	Binding	Inhibition of mouse PI3Kalpha after 30 mins by fluorescence polarization assay	Mus musculus	11	ALA1938337	single protein format	Scientific Literature
3.	ALA1039894	Binding	Inhibition of mouse recombinant PI3Kalpha expressed in baculovirus-infected Sf21 cells at 100 uM	Mus musculus	8	ALA1156056	cell-based format	Scientific Literature
4.	ALA1039898	Binding	Inhibition of mouse recombinant PI3Kalpha expressed in baculovirus-infected Sf21 cells	Mus musculus	8	ALA1156056	cell-based format	Scientific Literature
5.	ALA2021234	Binding	Inhibition of mouse wild type PI3Kalpha expressed in Sf21 cells co-expressing N-terminal His-tagged human p85a using L-alpha-phosphatidylinositol substrate by TLC based phosphor imaging	Mus musculus	7	ALA2016554	cell-based format	Scientific Literature
6.	ALA2061273	Binding	Inhibition of mouse PI3Kalpha	Mus musculus	12	ALA2057195	single protein format	Scientific Literature
7.	ALA2343996	Binding	Inhibition of mouse PI3Kalpha	Mus musculus	13	ALA2331555	single protein format	Scientific Literature
8.	ALA2379607	Binding	Inhibition of mouse PI3Kalpha	Mus musculus	25	ALA2375270	single protein format	Scientific Literature
9.	ALA3227714	Binding	Inhibition of mouse PI3K alpha expressed in insect cells coexpressing p85 subunit assessed as PI(3,4,5)P3 formation using PI(4,5)P2 as substrate by measuring displacement of TAMRA-labeled PI(3,4,5)P3 from Grip1 PH domain after 35 mins by ligand displacement fluorescence polarization assay	Mus musculus	9	ALA3217449	cell-based format	Scientific Literature
10.	ALA3887342	Binding	Cell Based Assay: This assay was used to measure PI3K-alpha inhibition in cells. BT474 cells (human breast ductal carcinoma, ATCC HTB-20) were seeded into black 384 well plates (Costar, #3712) at a density of 5600 cells/well in DMEM containing 10% FBS and 1% glutamine and allowed to adhere overnight.The following morning compounds in 100% DMSO were added to assay plates by acoustic dispensing. After a 2 hour incubation at 37 C. and 5% CO2, the medium was aspirated and the cells were lysed with a buffer containing 25 mM Tris, 3 mM EDTA, 3 mM EGTA, 50 mM sodium fluoride, 2 mM Sodium orthovanadate, 0.27M sucrose, 10 mM beta-glycerophosphate, 5 mM sodium pyrophosphate, 0.5% Triton X-100 and complete protease inhibitor cocktail tablets (Roche #04 693 116 001, used 1 tab per 50 ml lysis buffer).After 20 minutes, the cell lysates were transferred into ELISA plates (Greiner #781077) which had been pre-coated with an anti total-AKT antibody in PBS buffer.		9	ALA3886284	cell-based format	BindingDB Database
11.	ALA4156838	Binding	Inhibition of PI3Kalpha in mouse NIH-3T3 cells assessed as reduction in PDGF-BB-induced phosphorylation of AKT at Thr308 residues preincubated for 1 hr followed by PDGF-BB stimulation and measured after 10 mins by PAGE analysis	Mus musculus	1	ALA4152344	cell-based format	Scientific Literature
12.	ALA4705417	Binding	Inhibition of PI3Kalpha in mouse NIH/3T3 cells assessed as reduction in PDGF-BB stimulated AKT T308 phosphorylation up to 3 uM incubated for 1 hr by Western blot analysis	Mus musculus	1	ALA4699641	cell-based format	Scientific Literature
13.	ALA4765901	Binding	Inhibition of PI3K p110alpha in mouse embryonic fibroblast cells assessed as reduction in PDGF-induced AKT phosphorylation at S473 residue preincubated for 2 hrs followed by PDGF stimulation and measured after 10 mins	Mus musculus	1	ALA4765230	tissue-based format	Scientific Literature
14.	ALA5167639	Binding	Inhibition of PI3Kalpha in IGF-1 stimulated mouse C2C12 cells incubated for 10 mins by AlphaLISA assay	Mus musculus	4	ALA5154925	cell-based format	Scientific Literature
15.	ALA5225390	Binding	Inhibition of mouse PI3Kalpha expressed in insect cells assessed as fluorescence polarization incubated for 30 mins by ATP competitive assay	Mus musculus	1	ALA5223150	cell-based format	Scientific Literature