#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA2318052	B	Binding affinity to 3-PGDH in human HEI-193 cells harboring NF2 mutant at 100 uM after 30 mins by SDS-PAGE analysis	Homo sapiens	1	ALA2311428	cell-based format	Scientific Literature
2.	ALA3803742	B	Binding affinity to PHGDH in HEK293 proteomes at 20 uM incubated for 16 hrs by TAMRA biotin-azide dye based LC-MS/MS analysis	Homo sapiens	1	ALA3797061	cell-based format	Scientific Literature
3.	ALA3993752	B	Non-competitive inhibition of His6-tagged full length PHGDH (unknown origin) expressed in Escherichia coli BL21 (DE3) assessed as reduction in NADH formation using 3-phosphoglycerate as substrate preincubated for 30 mins followed by substrate addition in presence of NAD+ by fluorescence assay	Homo sapiens	1	ALA3992533	assay format	Scientific Literature
4.	ALA3993753	B	Binding affinity to PHGDH (unknown origin) by SPR assay	Homo sapiens	1	ALA3992533	single protein format	Scientific Literature
5.	ALA3993754	B	Non-competitive inhibition of PHGDH (unknown origin) assessed as reduction in NADH formation using 3-phosphoglycerate as substrate after 20 mins in presence of NAD+ by resorufin fluorescence based PSAT1/diaphorase/PSPH coupled enzyme assay	Homo sapiens	1	ALA3992533	single protein format	Scientific Literature
6.	ALA3993755	B	Inhibition of recombinant human C-terminal His-tagged PHGDH (1 to 533 residues) expressed in Escherichia coli assessed as reduction in NADH formation at 100 uM using 3-phosphoglycerate as substrate preincubated measured for 7 mins in presence of NAD+ by spectrophotometric method relative to control	Homo sapiens	15	ALA3992533	assay format	Scientific Literature
7.	ALA3993756	B	Inhibition of recombinant human C-terminal His-tagged PHGDH (1 to 533 residues) expressed in Escherichia coli assessed as reduction in NADH formation using 3-phosphoglycerate as substrate in presence of NAD+ by resorufin fluorescence based PSAT1/diaphorase/PSPH coupled enzyme assay	Homo sapiens	19	ALA3992533	assay format	Scientific Literature
8.	ALA3993758	B	Inhibition of PHGDH in human SiHa cells assessed as reduction in cell viability in serine repleted medium after 5 days by presto blue assay	Homo sapiens	19	ALA3992533	cell-based format	Scientific Literature
9.	ALA3993759	B	Inhibition of PHGDH in human SiHa cells assessed as reduction in cell viability in serine depleted medium after 5 days by presto blue assay	Homo sapiens	19	ALA3992533	cell-based format	Scientific Literature
10.	ALA3993760	B	Ratio of IC50 for inhibition of PHGDH in human SiHa cells assessed as reduction in cell viability in serine repleted medium to IC50 for inhibition of PHGDH in human SiHa cells assessed as reduction in cell viability in serine depleted medium	Homo sapiens	17	ALA3992533	cell-based format	Scientific Literature
11.	ALA3993761	B	Inhibition of PHGDH in human HL60 cells assessed as reduction in cell viability in serine repleted medium after 5 days by presto blue assay	Homo sapiens	19	ALA3992533	cell-based format	Scientific Literature
12.	ALA3993762	B	Inhibition of PHGDH in human HL60 cells assessed as reduction in cell viability in serine depleted medium after 5 days by presto blue assay	Homo sapiens	19	ALA3992533	cell-based format	Scientific Literature
13.	ALA3993763	B	Ratio of IC50 for inhibition of PHGDH in human HL60 cells assessed as reduction in cell viability in serine repleted medium to IC50 for inhibition of PHGDH in human HL60 cells assessed as reduction in cell viability in serine depleted medium	Homo sapiens	17	ALA3992533	cell-based format	Scientific Literature
14.	ALA3993765	B	Non-competitive inhibition of recombinant human C-terminal His-tagged PHGDH (1 to 533 residues) expressed in Escherichia coli assessed as reduction in NADH formation preincubated for 30 mins followed by varying levels of 3-phosphoglycerate substrate addition in presence of constant NAD+ concentration by resorufin fluorescence based PSAT1/diaphorase/PSPH coupled enzyme assay	Homo sapiens	1	ALA3992533	assay format	Scientific Literature
15.	ALA3993766	B	Non-competitive inhibition of recombinant human C-terminal His-tagged PHGDH (1 to 533 residues) expressed in Escherichia coli assessed as reduction in NADH formation preincubated for 30 mins followed by constant concentration of 3-phosphoglycerate substrate addition in presence of varying levels of NAD+ by resorufin fluorescence based PSAT1/diaphorase/PSPH coupled enzyme assay	Homo sapiens	1	ALA3992533	assay format	Scientific Literature
16.	ALA3993767	B	Covalent inhibition of human C-terminal His-tagged PHGDH (1 to 533 residues) expressed in Escherichia coli at 160 uM using 3-phosphoglycerate as substrate preincubated for 45 mins followed by 10-fold compound dilution in presence of NAD+ by resorufin fluorescence based PSAT1/diaphorase/PSPH coupled enzyme assay	Homo sapiens	1	ALA3992533	assay format	Scientific Literature
17.	ALA3998476	B	Binding affinity to PHGDH (unknown origin) by surface plasma resonance method	Homo sapiens	1	ALA3997775	single protein format	Scientific Literature
18.	ALA3998477	B	Inhibition of human liver N-terminal His6-tagged PHGDH expressed in Escherichia coli Rosetta (DE3)pLysS using 3 -phosphoglycerate as substarte measured at 20 mins by resazurin based diaphorase coupled assay	Homo sapiens	6	ALA3997775	assay format	Scientific Literature
19.	ALA3998478	B	Inhibition of PHGDH (unknown origin) by FLINT method	Homo sapiens	1	ALA3997775	single protein format	Scientific Literature
20.	ALA3998481	B	Inhibition of PHGDH (unknown origin) expressed in Escherichia coli BL21 using 3-phosphoglycerate as substrate preincubated for 4 hrs followed by substrate addition in presence of PSAT1 by NADH fluorescence assay	Homo sapiens	1	ALA3997775	assay format	Scientific Literature