| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA2318052 | B | Binding affinity to 3-PGDH in human HEI-193 cells harboring NF2 mutant at 100 uM after 30 mins by SDS-PAGE analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 2. | ALA3803742 | B | Binding affinity to PHGDH in HEK293 proteomes at 20 uM incubated for 16 hrs by TAMRA biotin-azide dye based LC-MS/MS analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 3. | ALA3993752 | B | Non-competitive inhibition of His6-tagged full length PHGDH (unknown origin) expressed in Escherichia coli BL21 (DE3) assessed as reduction in NADH formation using 3-phosphoglycerate as substrate preincubated for 30 mins followed by substrate addition in presence of NAD+ by fluorescence assay | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 4. | ALA3993753 | B | Binding affinity to PHGDH (unknown origin) by SPR assay | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 5. | ALA3993754 | B | Non-competitive inhibition of PHGDH (unknown origin) assessed as reduction in NADH formation using 3-phosphoglycerate as substrate after 20 mins in presence of NAD+ by resorufin fluorescence based PSAT1/diaphorase/PSPH coupled enzyme assay | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 6. | ALA3993755 | B | Inhibition of recombinant human C-terminal His-tagged PHGDH (1 to 533 residues) expressed in Escherichia coli assessed as reduction in NADH formation at 100 uM using 3-phosphoglycerate as substrate preincubated measured for 7 mins in presence of NAD+ by spectrophotometric method relative to control | Homo sapiens | 15 | assay format | Scientific Literature | ||
| 7. | ALA3993756 | B | Inhibition of recombinant human C-terminal His-tagged PHGDH (1 to 533 residues) expressed in Escherichia coli assessed as reduction in NADH formation using 3-phosphoglycerate as substrate in presence of NAD+ by resorufin fluorescence based PSAT1/diaphorase/PSPH coupled enzyme assay | Homo sapiens | 19 | assay format | Scientific Literature | ||
| 8. | ALA3993758 | B | Inhibition of PHGDH in human SiHa cells assessed as reduction in cell viability in serine repleted medium after 5 days by presto blue assay | Homo sapiens | 19 | cell-based format | Scientific Literature | ||
| 9. | ALA3993759 | B | Inhibition of PHGDH in human SiHa cells assessed as reduction in cell viability in serine depleted medium after 5 days by presto blue assay | Homo sapiens | 19 | cell-based format | Scientific Literature | ||
| 10. | ALA3993760 | B | Ratio of IC50 for inhibition of PHGDH in human SiHa cells assessed as reduction in cell viability in serine repleted medium to IC50 for inhibition of PHGDH in human SiHa cells assessed as reduction in cell viability in serine depleted medium | Homo sapiens | 17 | cell-based format | Scientific Literature | ||
| 11. | ALA3993761 | B | Inhibition of PHGDH in human HL60 cells assessed as reduction in cell viability in serine repleted medium after 5 days by presto blue assay | Homo sapiens | 19 | cell-based format | Scientific Literature | ||
| 12. | ALA3993762 | B | Inhibition of PHGDH in human HL60 cells assessed as reduction in cell viability in serine depleted medium after 5 days by presto blue assay | Homo sapiens | 19 | cell-based format | Scientific Literature | ||
| 13. | ALA3993763 | B | Ratio of IC50 for inhibition of PHGDH in human HL60 cells assessed as reduction in cell viability in serine repleted medium to IC50 for inhibition of PHGDH in human HL60 cells assessed as reduction in cell viability in serine depleted medium | Homo sapiens | 17 | cell-based format | Scientific Literature | ||
| 14. | ALA3993765 | B | Non-competitive inhibition of recombinant human C-terminal His-tagged PHGDH (1 to 533 residues) expressed in Escherichia coli assessed as reduction in NADH formation preincubated for 30 mins followed by varying levels of 3-phosphoglycerate substrate addition in presence of constant NAD+ concentration by resorufin fluorescence based PSAT1/diaphorase/PSPH coupled enzyme assay | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 15. | ALA3993766 | B | Non-competitive inhibition of recombinant human C-terminal His-tagged PHGDH (1 to 533 residues) expressed in Escherichia coli assessed as reduction in NADH formation preincubated for 30 mins followed by constant concentration of 3-phosphoglycerate substrate addition in presence of varying levels of NAD+ by resorufin fluorescence based PSAT1/diaphorase/PSPH coupled enzyme assay | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 16. | ALA3993767 | B | Covalent inhibition of human C-terminal His-tagged PHGDH (1 to 533 residues) expressed in Escherichia coli at 160 uM using 3-phosphoglycerate as substrate preincubated for 45 mins followed by 10-fold compound dilution in presence of NAD+ by resorufin fluorescence based PSAT1/diaphorase/PSPH coupled enzyme assay | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 17. | ALA3998476 | B | Binding affinity to PHGDH (unknown origin) by surface plasma resonance method | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 18. | ALA3998477 | B | Inhibition of human liver N-terminal His6-tagged PHGDH expressed in Escherichia coli Rosetta (DE3)pLysS using 3 -phosphoglycerate as substarte measured at 20 mins by resazurin based diaphorase coupled assay | Homo sapiens | 6 | assay format | Scientific Literature | ||
| 19. | ALA3998478 | B | Inhibition of PHGDH (unknown origin) by FLINT method | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 20. | ALA3998481 | B | Inhibition of PHGDH (unknown origin) expressed in Escherichia coli BL21 using 3-phosphoglycerate as substrate preincubated for 4 hrs followed by substrate addition in presence of PSAT1 by NADH fluorescence assay | Homo sapiens | 1 | assay format | Scientific Literature |
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