| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA2329320 | B | Inhibition of human GNT2 GalNAc-transferase using GnMan3-octyl as substrate at 0.5 mM after 1 hr in presence of phosphatidylcholine | Homo sapiens | 3 | single protein format | Scientific Literature | ||
| 2. | ALA2378803 | B | Inhibition of MGAT2 in human intestinal microsomes using 2-oleyl-sn-glycerol and [14C]-oleyl-coenzyme A as substrate after 10 to 15 mins by liquid scintillation counting analysis | Homo sapiens | 1 | microsome format | Scientific Literature | ||
| 3. | ALA2378971 | B | Inhibition of human recombinant MGAT2 expressed in insect cell membrane using 2-oleoyl-sn-glycerol and oleoyl coenzyme A as substrate after 40 mins by Ellmans assay based rapidfire LCMS analysis | Homo sapiens | 29 | cell-based format | Scientific Literature | ||
| 4. | ALA3583803 | B | In vitro inhibition of full length human MGAT2 transfected in FreeStyle293 cells assessed as dioleoylglycerol by RapidFire/MS assay | Homo sapiens | 25 | cell-based format | Scientific Literature | ||
| 5. | ALA3616241 | B | Inhibition of MGAT2 (unknown origin) assessed as effect on incorporation of [1-14C]decanoyl moiety into DAG using [1-14C]decanoyl-CoA and 1-decanoyl-rac-glycerol as substrates | Homo sapiens | 2 | single protein format | Scientific Literature | ||
| 6. | ALA3737416 | B | Inhibition of MGAT2 (unknown origin) by CPM assay | Homo sapiens | 15 | single protein format | Scientific Literature | ||
| 7. | ALA3737424 | B | Inhibition of human MGAT2 by CPM assay | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 8. | ALA3887138 | B | MGAT SPA Assay: MGAT2 enzyme was assayed using membranes isolated from Sf9 cells expressing the recombinant human MGAT2 cDNA with 2-monooleoylglycerol and [3H]-oleoyl-CoA as substrates as described by Seethala et al. [Anal. Biochem., 383(2):144-150 (Dec. 15, 2008)]. Briefly, the assays were conducted in 384-well plates in a total volume of 30 uL at 25° C. In each assay, 200 ng of recombinant human MGAT2 membrane was incubated with 10 uM of 2-monooleoylglycerol and 15 uM of [3H]-oleoyl-CoA in 100 mM potassium phosphate (pH 7.4) for 20 min with various concentrations of compounds delivered in DMSO. The assay was terminated by the addition of 20 ul of Stopping Solution (7.5 mg/ml Yttrium Oxide Polylysine beads, 3.3 mg/ml Fraction V BSA and 200 uM Mercuric chloride in 50 mM HEPES, pH 7.4). The signal was measured 1 h after quenching the reaction using LEADSEEKERSM for 5 minutes. To calculate the degree of inhibition, the zero level of enzyme activity (blank) was defined. | 127 | tissue-based format | BindingDB Database | |||
| 9. | ALA3887848 | B | MGAT LCMS Assay: The MGAT enzyme reactions were performed in CORNING Falcon 96-well Polypropylene plates, in a total volume of 60 uL of 50 mM Potassium Phosphate buffer pH 7.4, containing a final concentration of 100 uM 2-oleoylglycerol, 15 uM oleoyl-Coenzyme A and 0.0013 ug/uL Human or Mouse MGAT-2 or 0.0026 ug/uL Rat recombinant MGAT-2 membranes expressed in Sf9 cells. Assay plates were run through a fully automated robotics system and shaken for 5 seconds every minute for a total 10 minutes. The reactions were then quenched with 120 uL of ice cold methanol containing 1 ug/mL 1,2-distearoyl-rac-glycerol as the internal standard. Plates were shaken for 2 minutes and spun down to remove protein precipitation. After the spin, samples were transferred to LC/MS compatible PCR plates. For LC/MS analysis, a ThermoFisher Surveyor pump, utilizing a Waters Symmetry C8, 50x2.1 mm column, was used for the chromatography of enzyme products. | 262 | cell-based format | BindingDB Database | |||
| 10. | ALA3888582 | B | LCMS Assay: The MGAT enzyme reactions were performed in Corning FALCON® 96-well Polypropylene plates, in a total volume of 60 μL of 50 mM Potassium Phosphate buffer pH 7.4, containing a final concentration of 100 μM 2-oleoylglycerol, 15 μM oleoyl-Coenzyme A and 0.0013 μg/μL Human or Mouse MGAT-2 or 0.0026 μg/μL Rat recombinant MGAT-2 membranes expressed in Sf9 cells. Assay plates were run through a fully automated robotics system and shaken for 5 seconds every minute for a total 10 minutes. The reactions were then quenched with 120 μL of ice cold methanol containing 1 μg/mL 1,2-distearoyl-rac-glycerol as the internal standard. Plates were shaken for 2 minutes and spun down to remove protein precipitation. After the spin, samples were transferred to LC/MS compatible PCR plates. For LC/MS analysis, a ThermoFisher Surveyor pump, utilizing a Waters Symmetry C8, 50×2.1 mm column, was used for the chromatography of enzyme products. The buffer system consists of 0.1% formic acid in water with a mobile phase consisting 0.1% formic acid in methanol. The shallow gradient is 90-100% mobile phase in 0.2 min with a total run time of 2.3 min. The first 0.5 minutes of each injection was diverted to waste to eliminate the presence of Phosphate buffer in the enzymatic reaction. The column was run at 0.6 mL/min and a temperature of 65° C. | 93 | cell-based format | BindingDB Database | |||
| 11. | ALA4261861 | B | Inhibition of MGAT2 (unknown origin) | Homo sapiens | 2 | single protein format | Scientific Literature | ||
| 12. | ALA4371670 | B | Inhibition of recombinant human MGAT2 using 2-monooleoyl glycerol/[1-14C]-oleoyl CoA as substrate after 30 mins by liquid scintillation counting method | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 13. | ALA4371678 | B | Inhibition of recombinant human MGAT2 expressed in sf9 cells using 2-monooleoyl glycerol/[14C]-oleoyl CoA as substrate by microscintillation counting method | Homo sapiens | 3 | cell-based format | Scientific Literature | ||
| 14. | ALA4371681 | B | Inhibition of recombinant human MGAT2 | Homo sapiens | 15 | single protein format | Scientific Literature | ||
| 15. | ALA4845048 | B | Inhibition of human recombinant MGAT2 expressed in Sf9 cell membrane using 2-monooleglycerol and [H3]-oleoyl-CoA as substrates incubated for 20 mins by scintillation proximity assay | Homo sapiens | 59 | cell membrane format | Scientific Literature | ||
| 16. | ALA4883180 | B | RapidFire/MS assay (human MGAT2) | Homo sapiens | 2 | single protein format | Donated Chemical Probes - SGC Frankfurt | ||
| 17. | ALA4883186 | B | Cell-based assay | Homo sapiens | 1 | single protein format | Donated Chemical Probes - SGC Frankfurt | ||
| 18. | ALA5099710 | B | Inhibition of human MGAT2 using [1-14C]decanoyl-CoA and 1-decanoyl-rac-glycerol as substrates assessed as incorporation of [1-14C]decanoyl into diacylglycerol preincubated for 30 mins followed by substrate addition and measured after 30 to 90 mins by scintillation counting method | Homo sapiens | 2 | single protein format | Scientific Literature | ||
| 19. | ALA5145931 | B | Inhibition of recombinant human MGAT2 expressed in Sf9 cell membrane assessed as inhibition of triglyceride synthesis at 10 uM for 1 hr by LC-MS-based mass spectrophotometric analysis | Homo sapiens | 5 | cell membrane format | Scientific Literature | ||
| 20. | ALA5210220 | B | Affinity On-target Cellular interaction (Cell-based assay) EUB0000315b MGAT2 | Homo sapiens | 1 | single protein format | Literature data from EUbOPEN Chemogenomic Library |
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