| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA2330196 | B | Inhibition of SMYD3 (unknown origin) using histone H3-H4 tetramer as substrate after 1 hr by filter plate assay in presence of [3H]-S-adenosylmethionine | Homo sapiens | 1 | ALA2321656 | single protein format | Scientific Literature | |
| 2. | ALA3111733 | B | Inhibition of SMYD3 (unknown origin)-mediated incorporation of methyl group from [3H]-SAM into peptide substrate up to 50 uM by scintillation proximity assay | Homo sapiens | 1 | ALA3108684 | single protein format | Scientific Literature | |
| 3. | ALA3592421 | B | Inhibition of SMYD3 (unknown origin) assessed as incorporation of tritium labeled methyl group from [3H]-SAM to substrate at 1 uM after 1 hr by scintillation proximity assay relative to control | Homo sapiens | 1 | ALA3588844 | single protein format | Scientific Literature | |
| 4. | ALA3592425 | B | Inhibition of SMYD3 (unknown origin) assessed as incorporation of tritium labeled methyl group from [3H]-SAM to substrate at 10 to 50 uM after 1 hr by scintillation proximity assay relative to control | Homo sapiens | 1 | ALA3588844 | single protein format | Scientific Literature | |
| 5. | ALA3784099 | B | Inhibition of human SYMD3 at 10 and 50 uM | Homo sapiens | 1 | ALA3779843 | single protein format | Scientific Literature | |
| 6. | ALA3803119 | B | Inhibition of full length N-terminal His-tagged SMYD3 (1 to 428 residues) (unknown origin) expressed in Escherichia coli using N-terminal GST-tagged MEKK2 as substrate preincubated for 30 mins followed by substrate addition by scintillation proximity assay in presence of [3H]SAM | Homo sapiens | 20 | ALA3797202 | assay format | Scientific Literature | |
| 7. | ALA3803120 | B | Inhibition of human SMYD3 expressed in HEK293T/17 cells using FLAG-tagged MEKK2 as substrate incubated for 30 mins in low air flow area followed by incubation for 24 hrs at 37 degC by Western blot analysis | Homo sapiens | 20 | ALA3797202 | cell-based format | Scientific Literature | |
| 8. | ALA3803389 | B | Mixed type inhibition of full length N-terminal His-tagged SMYD3 (1 to 428 residues) (unknown origin) expressed in Escherichia coli using fixed N-terminal GST-tagged MEKK2 substrate and varying SAM levels preincubated for 30 mins followed by substrate addition by filter plate assay | Homo sapiens | 1 | ALA3797202 | assay format | Scientific Literature | |
| 9. | ALA3803390 | B | Non-competitive inhibition of full length N-terminal His-tagged SMYD3 (1 to 428 residues) (unknown origin) expressed in Escherichia coli using varying N-terminal GST-tagged MEKK2 substrate and fixed SAM levels preincubated for 30 mins followed by substrate addition by filter plate assay | Homo sapiens | 2 | ALA3797202 | assay format | Scientific Literature | |
| 10. | ALA3803391 | B | Non-competitive inhibition of full length N-terminal His-tagged SMYD3 (1 to 428 residues) (unknown origin) expressed in Escherichia coli using fixed N-terminal GST-tagged MEKK2 substrate and varying SAM levels preincubated for 30 mins followed by substrate addition by filter plate assay | Homo sapiens | 1 | ALA3797202 | assay format | Scientific Literature | |
| 11. | ALA3821559 | B | Inhibition of SMYD3 (unknown origin) at 1 to 50 uM by scintillation proximity assay | Homo sapiens | 1 | ALA3817823 | single protein format | Scientific Literature | |
| 12. | ALA3859072 | B | Inhibition of SMYD3 (unknown origin) at 50 uM using biotin-labeled peptide as substrate and [3H]-SAM measured after 1 hr by scintillation proximity assay | Homo sapiens | 1 | ALA3856251 | single protein format | Scientific Literature | |
| 13. | ALA4005620 | B | Inhibition of SMYD3 (unknown origin) at 10 uM using biotin-labeled peptide as substrate and [3H]-SAM measured after 1 hr by scintillation proximity assay | Homo sapiens | 1 | ALA4004823 | single protein format | Scientific Literature | |
| 14. | ALA4005649 | B | Inhibition of SMYD3 (unknown origin) at 1 uM using biotin-labeled peptide as substrate and [3H]-SAM measured after 1 hr by scintillation proximity assay | Homo sapiens | 1 | ALA4004823 | single protein format | Scientific Literature | |
| 15. | ALA4306860 | B | Inhibition of SMYD3 (unknown origin) using MAP3K2 peptide as substrate pretreated for 30 mins followed by substrate addition and measured after 30 mins by MTase Glo reagent based luminescence assay | Homo sapiens | 27 | ALA4304770 | single protein format | Scientific Literature | |
| 16. | ALA4306869 | B | Irreversible inhibition of SMYD3 (unknown origin) using MAP3K2 peptide as substrate assessed as Kinact pretreated for 5 mins followed by substrate addition and measured after 60 mins by MTase Glo reagent based luminescence assay | Homo sapiens | 3 | ALA4304770 | single protein format | Scientific Literature | |
| 17. | ALA4306870 | B | Irreversible inhibition of SMYD3 (unknown origin) using MAP3K2 peptide as substrate pretreated for 5 mins followed by substrate addition and measured after 60 mins by MTase Glo reagent based luminescence assay | Homo sapiens | 3 | ALA4304770 | single protein format | Scientific Literature | |
| 18. | ALA4306871 | B | Irreversible inhibition of SMYD3 (unknown origin) using MAP3K2 peptide as substrate assessed as kinact/Ki ratio pretreated for 5 mins followed by substrate addition and measured after 60 mins by MTase Glo reagent based luminescence assay | Homo sapiens | 3 | ALA4304770 | single protein format | Scientific Literature | |
| 19. | ALA4306872 | B | Inhibition of SMYD3 in human HepG2 cells assessed as inhibition of cell proliferation incubated for 3 days by CellTiter-Glo luminescent assay | Homo sapiens | 7 | ALA4304770 | cell-based format | Scientific Literature | |
| 20. | ALA4306873 | B | Inhibition of SMYD3 in human HepG2 cells assessed as inhibition of anchorage-independent colony formation in 3D culture cells incubated for 1 to 2 weeks by thiazolyl blue tetrazolium bromide based | Homo sapiens | 7 | ALA4304770 | cell-based format | Scientific Literature |
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