| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA2343949 | B | Inhibition of IPFK2 (unknown origin) | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 2. | ALA3106861 | B | Inhibition of PFKFB3-mediated glycolysis in human MDA-MB-231 cells assessed as extracellular acidification rate at 10 uM after 60 mins | Homo sapiens | 4 | cell-based format | Scientific Literature | ||
| 3. | ALA3106863 | B | Inhibition of PFKFB3-mediated glycolysis in human MDA-MB-231 cells assessed as extracellular acidification rate at 100 uM after 2 hrs | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 4. | ALA3106864 | B | Inhibition of PFKFB3-mediated glycolysis in human MDA-MB-231 cells assessed as extracellular acidification rate at 10 uM after 2 hrs | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 5. | ALA3106865 | B | Inhibition of human recombinant GST-tagged PFKFB3 expressed in Escherichia coli BL21(DE3) using fructose 6-phosphate as substrate at 250 uM preincubated for 30 mins before substrate addition measured after 60 mins by kinase-glo assay | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 6. | ALA3106983 | B | Inhibition of human recombinant GST-tagged PFKFB3 expressed in Escherichia coli BL21(DE3) using fructose 6-phosphate as substrate preincubated for 30 mins before substrate addition measured after 60 mins by kinase-glo assay | Homo sapiens | 23 | assay format | Scientific Literature | ||
| 7. | ALA3106984 | B | Inhibition of PFKFB3 in human HeLa cells assessed as reduction of fructose 2,6-bisphosphate level at IC50 after 8 hrs | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 8. | ALA3106985 | B | Inhibition of PFKFB3 in human HeLa cells assessed as reduction of lactate secretion at IC50 after 8 hrs | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 9. | ALA3106986 | B | Competitive inhibition of PFKFB3 (unknown origin) using fructose 6-phosphate as substrate | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 10. | ALA3106987 | B | Inhibition of PFKFB3 (unknown origin) | Homo sapiens | 5 | single protein format | Scientific Literature | ||
| 11. | ALA3386523 | B | Inhibition of PFKFB3 (unknown origin) | Homo sapiens | 3 | single protein format | Scientific Literature | ||
| 12. | ALA3425140 | B | Inhibition of recombinant human PFKFB3 using fructose 6 phosphate as substrate assessed as ADP generation after 1 hr by ADP Glo assay | Homo sapiens | 38 | single protein format | Scientific Literature | ||
| 13. | ALA3425231 | B | Inhibition of PFKFB3 in human A549 cells assessed as reduction of fructose-1,6-bisphosphate formation after 4 hrs by MS/MS analysis | Homo sapiens | 20 | cell-based format | Scientific Literature | ||
| 14. | ALA3425232 | B | Inhibition of PFKFB3 in human A549 cells assessed as decrease in lactate secretion after 4 hrs | Homo sapiens | 32 | cell-based format | Scientific Literature | ||
| 15. | ALA3425233 | B | Ratio of IC50 for PFKFB3-induced fructose-1,6-bisphosphate formation in human A549 cells to IC50 for recombinant human PFKFB3 | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 16. | ALA3425295 | B | Ratio of IC50 for PFKFB3-induced lactate formation in human A549 cells to IC50 for recombinant human PFKFB3 | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 17. | ALA3721246 | B | Inhibition of human PFKFB3 expressed in Escherichia coli BL21 (DE3) assessed as reduction in NADH consumption using fructose-6-phosphate incubated for 15 mins by spectrophotometry based pyruvate kinase and LDH enzyme coupled kinetic assay | Homo sapiens | 6 | assay format | Patent Bioactivity Data | ||
| 18. | ALA3721247 | B | Mixed type inhibition of human PFKFB3 expressed in Escherichia coli BL21 (DE3) assessed as reduction in NADH consumption using fructose-6-phosphate incubated for 15 mins by spectrophotometry based pyruvate kinase and LDH enzyme coupled kinetic assay | Homo sapiens | 1 | assay format | Patent Bioactivity Data | ||
| 19. | ALA3721256 | B | Inhibition of recombinant PFKFB3 (unknown origin) assessed as enzyme kinetic rate at 150 uM relative to control | Homo sapiens | 1 | single protein format | Patent Bioactivity Data | ||
| 20. | ALA3721257 | B | Mixed type inhibition of human PFKFB3 expressed in Escherichia coli BL21 (DE3) assessed as reduction in NADH consumption in presence of high levels of fructose-6-phosphate incubated for 15 mins by spectrophotometry based pyruvate kinase and LDH enzyme coupled kinetic assay | Homo sapiens | 1 | assay format | Patent Bioactivity Data |
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