# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA3539028 | B | Inhibition of DHCR24 activity in human hepatocytes assessed as increase in desmosterol level per gram of protein by GC-MS analysis (Rvb = 5.5 ug) | Homo sapiens | 1 | ALA3526087 | cell-based format | Scientific Literature | |
2. | ALA3779137 | B | Inhibition of DR24 in human SLOS fibroblasts assessed as decrease in 7-DHC levels at 10 nM after 5 days by LC-MS/GC-MS analysis | Homo sapiens | 6 | ALA3774374 | single protein format | Scientific Literature | |
3. | ALA3776542 | B | Inhibition of DR24 in human SLOS fibroblasts assessed as decrease in 7-DHC levels at 100 nM after 5 days by LC-MS/GC-MS analysis | Homo sapiens | 1 | ALA3774374 | single protein format | Scientific Literature | |
4. | ALA3991691 | B | Kinobeads (epsilon), multiple immobilized ATP-competitive broad spectrum kinase inhibitors, used to assess residual binding of ~300 proteins simultaneously from cell lysate in the presence of a compound. Quantitative readout performed by mass spectrometry. | Homo sapiens | 239 | ALA3991601 | subcellular format | Kuster lab chemical proteomics drug profiling | |
5. | ALA4134391 | B | Inhibition of DHCR24 in human HL60 cells assessed as compound level causing induction of sterol accumulation incubated for 24 hrs by GC-MS analysis | Homo sapiens | 19 | ALA4130461 | cell-based format | Scientific Literature | |
6. | ALA4134393 | B | Inhibition of DHCR24-mediated cholesterol biosynthesis in human HL60 cells assessed as inhibition of 2-[13C]acetate incorporation into newly synthesized cholesterol after 24 hrs GC/MS analysis | Homo sapiens | 28 | ALA4130461 | cell-based format | Scientific Literature | |
7. | ALA4729480 | B | Displacement of polymer supported probe 25 binding to DHCR24 in human THP-1 cell lysates at 30 uM preincubated for 45 mins under shaking condition followed by probe addition and measured after 30 mins by mass spectrometry based chemoproteomic analysis relative to control | Homo sapiens | 1 | ALA4725370 | subcellular format | Scientific Literature |