# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA3807566 | Binding | Inhibition of mouse wild type BRAF assessed as reduction in human full length N-terminal His-tagged MEK1 phosphorylation pre-incubated for 1 hr before MEK1 addition and measured after 25 mins | Mus musculus | 6 | ALA3804763 | single protein format | Scientific Literature | |
2. | ALA2342631 | Binding | Inhibition of wild type BRAF in mouse NIH/3T3 cells assessed as decrease in ERK phosphorylation after 75 mins by Western blotting analysis | Mus musculus | 2 | ALA2331403 | cell-based format | Scientific Literature | |
3. | ALA4195691 | Binding | Inhibition of full-length GST-tagged mouse BRAF V600E mutant using recombinant human full length N-terminal His-tagged MEK1 as substrate preincubated for 1 hr followed by substrate addition measured after 25 mins by Nu-page gel-based phosphor screen analysis | Mus musculus | 25 | ALA4190403 | single protein format | Scientific Literature | |
4. | ALA4196286 | Binding | Inhibition of mouse full-length GST-tagged BRAF V600E mutant using recombinant human full length N-terminal His-tagged MEK1 as substrate preincubated for 60 mins followed by substrate addition and measured after 25 mins by ELISA | Mus musculus | 7 | ALA4196002 | single protein format | Scientific Literature | |
5. | ALA4222975 | Binding | Inhibition of mouse full length GST-tagged BRAF V600E mutant using recombinant human full length N-terminal His-tagged MEK1 as substrate preincubated for 1 hr followed by substrate addition measured after 25 mins by Nu-page gel-based phosphor screen analysis | Mus musculus | 21 | ALA4219184 | single protein format | Scientific Literature | |
6. | ALA4232586 | Binding | Induction of N-terminal 6x-His-tagged BRAF V600E/R509H mutant (unknown origin) dimer formation at 12 uM by sedimentation velocity method | Homo sapiens | 1 | ALA4229397 | single protein format | Scientific Literature | |
7. | ALA4232606 | Binding | Induction of N-terminal 6x-His-tagged BRAF R509H/R506A mutant (unknown origin) dimer formation assessed as molecular weight of protein-compound complex at 20 uM by sedimentation equilibrium method | Homo sapiens | 1 | ALA4229397 | single protein format | Scientific Literature | |
8. | ALA4232607 | Binding | Induction of N-terminal 6x-His-tagged BRAF R509H/W450A mutant (unknown origin) dimer formation assessed as molecular weight of protein-compound complex at 20 uM by sedimentation equilibrium method | Homo sapiens | 1 | ALA4229397 | single protein format | Scientific Literature | |
9. | ALA4272239 | Binding | Inhibition of mouse full length GST-tagged BRAF V600E mutant using recombinant human full length N-terminal His-tagged MEK1 as substrate preincubated for 1 hr followed by substrate addition measured after 25 mins | Mus musculus | 17 | ALA4270537 | single protein format | Scientific Literature | |
10. | ALA4273764 | Binding | Inhibition of mouse full length GST-tagged BRAF V600E mutant using recombinant human His6-tagged MEK1 as substrate preincubated for 1 hr followed by substrate addition measured after 25 mins by ELISA | Mus musculus | 2 | ALA4270577 | single protein format | Scientific Literature | |
11. | ALA5231087 | Binding | Inhibition of full length GST tagged mouse wild type B-Raf preincubated for 1 hr and measured after 25 mins in presence of ATP | Mus musculus | 2 | ALA5230104 | single protein format | Scientific Literature |