# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA2343086 | B | Inhibition of SHIP2 in TNF alpha-treated mouse 3T3L1 cells assessed as potentiation of insulin-stimulated Akt phosphorylation at Thr308 at 10 uM treated for 15 mins after TNF alpha challenge for 16 hrs and prior to insulin stimulation measured after 2 hrs by Western blot analysis relative to untreated control | Mus musculus | 2 | ALA2331279 | cell-based format | Scientific Literature | |
2. | ALA2343087 | B | Inhibition of SHIP2 in mouse 3T3L1 cells assessed as reduction of TNF-alpha induced decrease in insulin-stimulated Akt phosphorylation at Thr308 at 10 uM treated for 15 mins after TNF alpha challenge for 16 hrs and prior to insulin stimulation measured after 2 hrs by Western blot analysis | Mus musculus | 12 | ALA2331279 | cell-based format | Scientific Literature | |
3. | ALA4274868 | B | Inhibition of SHIP2 in mouse HT22 cells assessed as decrease in PI(3,4)P2 production at 10 uM after 24 hrs by mKate2-TAPP1-PH based fluorescence imaging method | Mus musculus | 3 | ALA4270607 | cell-based format | Scientific Literature | |
4. | ALA4274869 | B | Inhibition of SHIP2 in H2O2-stimulated mouse HT22 cells assessed as decrease in PI(3,4)P2 production at 10 uM incubated for 30 mins followed by H2O2 stimulation for 5 mins in presence of PTEN inhibitor VO-Ohpic trihydrate by ELISA | Mus musculus | 1 | ALA4270607 | assay format | Scientific Literature | |
5. | ALA5053568 | B | Inhibition of SHIP2 in serum-starved mouse Mm1 cells assessed as reduction in Akt phosphorylation incubated for 30 mins by Western blot analysis | Mus musculus | 3 | ALA5053481 | tissue-based format | Scientific Literature |