Inhibition of SHIP2 (unknown origin) assessed as remaining enzyme activity at 30 uM using PtdIns(3,4,5)P3 as substrate preincubated for 20 mins followed by substrate addition measured for 50 mins by malachite green staining based assay relative to control
Inhibition of SHIP2 (unknown origin) assessed as decrease in PIP2 production using PtdIns(3,4,5)P3 as substrate preincubated for 20 mins followed by substrate addition measured for 50 mins by malachite green staining based assay
Displacement of 2FAMInsP5 from recombinant human N-terminal His-tagged SHIP2 (419 to 832 residues) expressed in Escherichia coli Rosetta2 (DE3) cells by fluorescence polarization assay
Binding affinity to recombinant human N-terminal His-tagged SHIP2 (419 to 832 residues) expressed in Escherichia coli Rosetta2 (DE3) cells assessed as increase in release of phosphate at 100 uM incubated for 10 mins by phosphate release assay
Inhibition of His-tagged SHIP2 (unknown origin) incubated for 5 mins using Ins(1,3,4,5)P4 as substrate by malachite green reagent based phosphate assay
Substrate activity at recombinant human N-terminal His-tagged SHIP2 (419 to 832 residues) expressed in Escherichia coli Rosetta2 (DE3) cells assessed as 5-OH-Bz(1,2,4)P3 product formation at 100 uM incubated for 12 hrs by fluorescence based HPLC analysis
Substrate activity at recombinant human N-terminal His-tagged SHIP2 (419 to 832 residues) expressed in Escherichia coli Rosetta2 (DE3) cells assessed as 5-OH-Bz(1,2,4)P3 product formation at 100 uM incubated for 2 hrs with 100 nM to 1 mM of SHIP2 by fluorescence based HPLC analysis
Substrate activity at recombinant human N-terminal His-tagged SHIP2 (419 to 832 residues) expressed in Escherichia coli Rosetta2 (DE3) cells assessed as 5-OH-Bz(1,2,4)P3 product formation at 100 uM measured upto 4 hrs by fluorescence based HPLC analysis
Substrate activity at recombinant human N-terminal His-tagged SHIP2 (419 to 832 residues) expressed in Escherichia coli Rosetta2 (DE3) cells assessed as 5-OH-Bz(1,2,4)P3 product formation at 100 uM incubated for 2 hrs by fluorescence based HPLC analysis
Substrate activity at recombinant human N-terminal His-tagged SHIP2 (419 to 832 residues) expressed in Escherichia coli Rosetta2 (DE3) cells assessed as 5-OH-Bz(1,2,4)P3 product formation at 100 uM incubated for 2 hrs in presence of INS(1,3,4,5)P4 by fluorescence based HPLC analysis
Substrate activity at recombinant human N-terminal His-tagged SHIP2 (419 to 832 residues) expressed in Escherichia coli Rosetta2 (DE3) cells assessed as 5-OH-Bz(1,2,4)P3 product formation at 100 uM incubated for 2 hrs in presence of AS1949490 by fluorescence based HPLC analysis
Substrate activity at recombinant human N-terminal His-tagged SHIP2 (419 to 832 residues) expressed in Escherichia coli Rosetta2 (DE3) cells assessed as 3-OH-Bz(1,2,4)P3 product formation at 100 uM incubated for 2 hrs in presence of AS1949490 by fluorescence based HPLC analysis
Substrate activity at recombinant human N-terminal His-tagged SHIP2 (419 to 832 residues) expressed in Escherichia coli Rosetta2 (DE3) cells assessed as 1,2-diOH-Bz(3,4)P2 product formation at 100 uM incubated for 2 hrs in presence of AS1949490 by fluorescence based HPLC analysis
Substrate activity at recombinant human N-terminal His-tagged SHIP2 (419 to 832 residues) expressed in Escherichia coli Rosetta2 (DE3) cells assessed as 1,3-Di-OH-Bz(2,4)P2 product formation at 100 uM incubated for 2 hrs in presence of AS1949490 by fluorescence based HPLC analysis
Inhibition of 2-FAM-InsP5 binding to human SHIP2 catalytic domain (419 to 832 residues) assessed as change in polarization by fluorescence polarization based displacement assay
Inhibition of human SHIP2 catalytic domain (419 to 832 residues) phosphatase activity assessed as phosphate release using Ins(1,3,4,5)P4 as substrate incubated for 20 mins measured using microplate reader