| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA2344107 | B | Inhibition of human hexahistidine-tagged full-length FTO expressed in Escherichia coli BL21 (DE3) using 3-methylthymidine as substrate assessed as inhibition of 3-methylthymidine conversion to thymidine after 1 hr by liquid chromatographic analysis | Homo sapiens | 33 | ALA2331261 | assay format | Scientific Literature | |
| 2. | ALA2344108 | B | Inhibition of human hexahistidine-tagged full-length FTO expressed in Escherichia coli BL21 (DE3) assessed as residual activity at 200 uM by scintillation proximity assay | Homo sapiens | 5 | ALA2331261 | assay format | Scientific Literature | |
| 3. | ALA2344109 | B | Binding affinity to human hexahistidine-tagged full-length FTO expressed in Escherichia coli BL21 (DE3) assessed as change in melting temperature at 200 uM after 80 mins by dynamic scanning fluorometry assay | Homo sapiens | 8 | ALA2331261 | assay format | Scientific Literature | |
| 4. | ALA3614714 | B | Inhibition of FTO R96Q (unknown origin) expressed in mouse 3T3L1 cells increase in demethylation of m6A modified mRNA levels at 10 uM incubated for 48 hrs by dot blot method | Homo sapiens | 1 | ALA3612002 | cell-based format | Scientific Literature | |
| 5. | ALA3614715 | B | Inhibition of FTO (unknown origin) (31 to 505 residues) expressed in Escherichia coli BL21 (DE3) cells assessed as reduction in 3-meT substrate demethylation by measuring demethylation ratio at 80 uM incubated for 17 hrs at 16 degC by HPLC method | Homo sapiens | 20 | ALA3612002 | cell-based format | Scientific Literature | |
| 6. | ALA3614716 | B | Inhibition of FTO (unknown origin) (31 to 505 residues) expressed in Escherichia coli BL21 (DE3) cells assessed as reduction in 15-mer ssRNA (5'-CUUGUCA(m6A)CAGCAGA-3') substrate demethylation by measuring demethylation ratio at 80 uM incubated for 0.5 hrs at room temperature by LC/MS method | Homo sapiens | 1 | ALA3612002 | cell-based format | Scientific Literature | |
| 7. | ALA3614717 | B | Binding affinity to FTO (unknown origin) (31 to 505 residues) expressed in Escherichia coli BL21 (DE3) by ITC method | Homo sapiens | 1 | ALA3612002 | assay format | Scientific Literature | |
| 8. | ALA3614719 | B | Inhibition of FTO (unknown origin) expressed in mouse 3T3L1 cells increase in demethylation of m6A modified mRNA levels at 10 uM incubated for 48 hrs by dot blot method | Homo sapiens | 1 | ALA3612002 | cell-based format | Scientific Literature | |
| 9. | ALA4418244 | B | Inhibition of FTO (unknown origin) assessed as reduction in demethylation using 39nt-m6 A-ssDNA and 2OG incubated for 2 hrs by non-denaturing polyacrylamide electrophoresis method | Homo sapiens | 27 | ALA4417641 | single protein format | Patent Bioactivity Data | |
| 10. | ALA4418739 | B | Inhibition of FTO (unknown origin) assessed as reduction in demethylation by measuring N6-mA and A level incubated for 2 hrs by HPLC-MS/MS analysis | Homo sapiens | 119 | ALA4418489 | single protein format | Patent Bioactivity Data | |
| 11. | ALA5115878 | B | Inhibition of human full lenth N-terminal His6-tagged FTO (1 to 505 residues) expressed in Escherichia coli BL21 (DE3) Rosetta cells | Homo sapiens | 1 | ALA5113412 | cell-based format | Scientific Literature | |
| 12. | ALA5115886 | B | Inhibition of FTO (unknown origin) mediated demethylation of m6A substrate at 0.1 to 10 uM incubated for 2 hrs by dot blot assay | Homo sapiens | 1 | ALA5113412 | single protein format | Scientific Literature | |
| 13. | ALA5115887 | B | Binding affinity to FTO (unknown origin) demethylation activity assessed as thermal stability by measuring change in melting temperature at 100 uM by differential scanning fluorimetry assay | Homo sapiens | 1 | ALA5113412 | single protein format | Scientific Literature | |
| 14. | ALA5115897 | B | Inhibition of FTO (unknown origin) mediated demethylation of m6A assessed as disruption of enzyme substrate binding by fluorescence polarisation assay | Homo sapiens | 1 | ALA5113412 | single protein format | Scientific Literature | |
| 15. | ALA5125059 | B | Inhibition of N-terminal His-tagged human FTO expressed in Escherichia coli BL21(DE3) at 20 uM relative to control | Homo sapiens | 33 | ALA5120916 | assay format | Scientific Literature | |
| 16. | ALA5125060 | B | Inhibition of N-terminal His-tagged human FTO expressed in Escherichia coli BL21(DE3) by PAGE based assay | Homo sapiens | 19 | ALA5120916 | assay format | Scientific Literature | |
| 17. | ALA5125061 | B | Binding affinity to N-terminal His-tagged human FTO expressed in Escherichia coli BL21(DE3) assessed as change in melting temperature at 1:5 protein to compound ratio by fluorescence based thermal shift assay | Homo sapiens | 2 | ALA5120916 | assay format | Scientific Literature | |
| 18. | ALA5125069 | B | Binding affinity to FTO in human MONO-MAC-6 cells assessed as thermal denaturation of protein at 40 to 50 uM by cellular thermal shift assay | Homo sapiens | 1 | ALA5120916 | cell-based format | Scientific Literature | |
| 19. | ALA5125070 | B | Inhibition of FTO in human MONO-MAC-6 cells assessed as upregulation of m6A total RNA level measured after 48 hrs by dot blotting analysis | Homo sapiens | 1 | ALA5120916 | cell-based format | Scientific Literature | |
| 20. | ALA5125071 | B | Inhibition of FTO in human MONO-MAC-6 cells assessed as upregulation of m6A total mRNA level measured after 48 hrs by dot blotting analysis | Homo sapiens | 1 | ALA5120916 | cell-based format | Scientific Literature |
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