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# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA3636364 | Binding | Inhibition of peroxidase activity of MPO isolated from human polynuclear leukocytes using Amplex Red as substrate assessed as formation of resorufin at 0.47 to 30 uM by spectrophotometric analysis | Homo sapiens | 1 | ALA3632461 | assay format | Scientific Literature | |
2. | ALA3636365 | Binding | Inhibition of peroxidase activity of MPO isolated from human polynuclear leukocytes using Amplex Red as substrate assessed as formation of resorufin after 300 secs by spectrophotometric analysis | Homo sapiens | 1 | ALA3632461 | assay format | Scientific Literature | |
3. | ALA3636366 | Binding | Irreversible inhibition of peroxidase activity of MPO isolated from human polynuclear leukocytes using Amplex Red as substrate assessed as formation of resorufin at 24 uM preincubated for 15 mins with H2O2 followed by 300 fold dilution measured immediately post dilution by spectrophotometric analysis | Homo sapiens | 1 | ALA3632461 | assay format | Scientific Literature | |
4. | ALA3636367 | Binding | Irreversible inhibition of peroxidase activity of MPO isolated from human polynuclear leukocytes using Amplex Red as substrate assessed as formation of resorufin at 24 uM preincubated for 15 mins without H2O2 followed by 300 fold dilution measured immediately post dilution by spectrophotometric analysis | Homo sapiens | 1 | ALA3632461 | assay format | Scientific Literature | |
5. | ALA3636507 | Binding | Inhibition of peroxidase activity of MPO isolated from human polynuclear leukocytes using Amplex Red as substrate assessed as partition ratio preincubated for 15 mins with H2O2 followed by 300 fold dilution measured immediately post dilution by spectrophotometric analysis | Homo sapiens | 5 | ALA3632461 | assay format | Scientific Literature | |
6. | ALA3636509 | Binding | Inhibition of peroxidase activity of MPO isolated from human polynuclear leukocytes using Amplex Red as substrate assessed as formation of resorufin measured every 20 secs by spectrophotometric analysis | Homo sapiens | 6 | ALA3632461 | assay format | Scientific Literature | |
7. | ALA3636510 | Binding | Inhibition of peroxidase activity of MPO isolated from human polynuclear leukocytes using Amplex Red as substrate assessed as formation of resorufin by measuring ratio of Kinact to Ki measured every 20 secs by spectrophotometric analysis | Homo sapiens | 9 | ALA3632461 | assay format | Scientific Literature | |
8. | ALA3636511 | Binding | Irreversible inhibition of MPO in LPS-stimulated human whole blood after 4 hrs by Amplex Red/H2O2-based fluorescence plate reader analysis | Homo sapiens | 9 | ALA3632461 | single protein format | Scientific Literature | |
9. | ALA3636512 | ADME | Drug metabolism assessed as formation of human MPO-mediated PTU-disulfide at 10 uM after 60 mins by LC-MS/MS analysis in presence of H2O2/Cl- | Homo sapiens | 1 | ALA3632461 | single protein format | Scientific Literature | |
10. | ALA3636513 | ADME | Drug metabolism assessed as formation of human MPO-mediated PTU-SO2- at 10 uM after 60 mins by LC-MS/MS analysis in presence of H2O2/Cl- | Homo sapiens | 1 | ALA3632461 | single protein format | Scientific Literature | |
11. | ALA3636514 | ADME | Drug metabolism assessed as formation of human MPO-mediated PTU-SO3- at 10 uM after 60 mins by LC-MS/MS analysis in presence of H2O2/Cl- | Homo sapiens | 1 | ALA3632461 | single protein format | Scientific Literature | |
12. | ALA3636516 | ADME | Drug metabolism assessed as formation of human MPO-mediated (S)-2-amino-5-((R)-1-(carboxymethylamino)-3-(6-(2,4-dimethoxyphenyl)-1-(2-isopropoxyethyl)-4-oxo-1,4-dihydropyrimidin-2-ylthio)-1-oxopropan-2-ylamino)-5-oxopentanoic acid at 10 uM after 60 mins by LC-MS/MS analysis in presence of H2O2/Cl-/excess GSH | Homo sapiens | 1 | ALA3632461 | single protein format | Scientific Literature | |
13. | ALA3744354 | Binding | Inhibition of human recombinant myeloperoxidase by amplex red assay | Homo sapiens | 36 | ALA3739290 | single protein format | Scientific Literature | |
14. | ALA3744355 | Binding | Inhibition of human recombinant myeloperoxidase upto 100 uM by amplex red assay | Homo sapiens | 36 | ALA3739290 | single protein format | Scientific Literature | |
15. | ALA3744356 | Binding | Inhibition of human recombinant myeloperoxidase by taurine chlorination assay | Homo sapiens | 4 | ALA3739290 | single protein format | Scientific Literature | |
16. | ALA3744360 | Binding | Non-competitive inhibition of human recombinant myeloperoxidase at 5 uM by amplex red assay using varying concentration of H2O2 | Homo sapiens | 2 | ALA3739290 | single protein format | Scientific Literature | |
17. | ALA3744361 | Binding | Competitive inhibition of human recombinant myeloperoxidase at 0.5 uM by amplex red assay using varying concentration of amplex red | Homo sapiens | 2 | ALA3739290 | single protein format | Scientific Literature | |
18. | ALA3744362 | Binding | Reversible inhibition of human recombinant myeloperoxidase at 6 uM for 1 hr followed by washout by ELISA in presence of 50 uM H2O2 | Homo sapiens | 1 | ALA3739290 | single protein format | Scientific Literature | |
19. | ALA3744363 | Binding | Reversible inhibition of human recombinant myeloperoxidase at 100 uM for 1 hr followed by washout by ELISA in presence of 50 uM H2O2 | Homo sapiens | 1 | ALA3739290 | single protein format | Scientific Literature | |
20. | ALA3744364 | Binding | Reversible inhibition of human recombinant myeloperoxidase at 50 uM for 1 hr followed by washout by ELISA in presence of 50 uM H2O2 | Homo sapiens | 2 | ALA3739290 | single protein format | Scientific Literature |
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